LOCUS       sequence-361421-        9483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination. May not contain 
            entire coding region of gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9483)
  TITLE     SGC Structures-2021-Q3 and Q4
REFERENCE   2  (bases 1 to 9483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: huntingtin:TOC031-A07:C246751
FEATURES             Location/Qualifiers
     source          1..9483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(545..550,551..567,568..573))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    1081..1129
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     1154..1173
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        1234..1613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1614..1817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1768..1788
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        1864..1973
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      4475..4484
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5152..5175
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5197..5226
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             5227..5250
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(5340..5360)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5758..5769)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5994..6128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6031..6050)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6085..6104
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6157..6322
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      6506..6960
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6593..6612)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6802..6823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6986..7090
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7091..7159,7160..7951)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7309..7328)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8122..8710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8611..8630
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8864..8881
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  9015..9239
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(9306..9483,1..356))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
       61 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
      121 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
      181 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
      241 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
      301 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
      361 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
      421 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
      481 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
      541 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
      601 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct
      661 ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt
      721 cttctacggc aaggtgctgt gcacggatct gcccttgctt caggagatcg gtagacctcg
      781 gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt
      841 tctggaaggc gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct
      901 acgtacccgt agtggctatg gcagggcttg ccgccccgac gttggctgcg agccctgggc
      961 cttcacccga acttgggggt tggggtgggg aaaaggaaga aacgcgggcg tattggtccc
     1021 aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat
     1081 gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
     1141 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tcccccatct cccggtaccg
     1201 catgcggatc tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     1261 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     1321 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     1381 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     1441 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     1501 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     1561 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     1621 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     1681 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     1741 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     1801 tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaat
     1861 acggaccttt aattcaaccc aacacaatat attatagtta aataagaatt attatcaaat
     1921 catttgtata ttaattaaaa tactatactg taaattacat tttatttaca atcactcgac
     1981 ggatccttaa gaaggagata tactatggtg agtccggaca aagactggta cgttcatctt
     2041 gtcaaatccc agtgttggac caggtcagat tctgcactgc tggaaggtgc agagctggtg
     2101 aatcggattc ctgctgaaga tatgaatgcc ttcatgatga actcggagtt caacctaagc
     2161 ctgctagctc catgcttaag cctagggatg agtgaaattt ctggtggcca gaagagtgcc
     2221 ctttttgaag cagcccgtga ggtgactctg gcccgtgtga gcggcaccgt gcagcagctc
     2281 cctgctgtcc atcatgtctt ccagcccgag ctgcctgcag aaccggcggc ctactggagc
     2341 aagttgaatg atctgtttgg ggatgctgca ctgtatcagt ccctgcccac tctggcccgg
     2401 gccctggcac agtacctggt ggtggtctcc aaactgccca gtcatttgca ccttcctcct
     2461 gagaaagaga aggacattgt gaaattcgtg gtggcaaccc ttgaggccct gtcctggcat
     2521 ttgatccatg agcagatccc gctgagtctg gatctccagg cagggctgga ctgctgctgc
     2581 ctggccctgc agctgcctgg cctctggagc gtggtctcct ccacagagtt tgtgacccac
     2641 gcctgctccc tcatccactg tgtgcacttc atcctggagg ccgttgcagt gcagcctgga
     2701 gagcagcttc ttagtccaga aagaaggaca aataccccaa aagccatcag cgaggaggag
     2761 gaggaagtag atccaaacac acagaatcct aagtatatca ctgcagcctg tgagatggtg
     2821 gcagaaatgg tggagtctct gcagtcggtg ttggccttgg gtcataaaag gaatagcggc
     2881 gtgccggcgt ttctcacgcc attgctcagg aacatcatca tcagcctggc ccgcctgccc
     2941 cttgtcaaca gctacacacg tgtgccccca ctggtgtgga agcttggatg gtcacccaaa
     3001 ccgggagggg attttggcac agcattccct gagatccccg tggagttcct ccaggaaaag
     3061 gaagtcttta aggagttcat ctaccgcatc aacacactag gctggaccag tcgtactcag
     3121 tttgaagaaa cttgggccac cctccttggt gtcctggtga cgcagcccct cgtgatggag
     3181 caggaggaga gcccaccaga agaagacaca gagaggaccc agatcaacgt cctggccgtg
     3241 caggccatca cctcactggt gctcagtgca atgactgtgc ctgtggccgg caacccagct
     3301 gtaagctgct tggagcagca gccccggaac aagcctctga aagctctcga caccaggttt
     3361 gggaggaagc tgagcattat cagagggatt gtggagcaag agattcaagc aatggtttca
     3421 aagagagaga atattgccac ccatcattta tatcaggcat gggatcctgt cccttctctg
     3481 tctccggcta ctacaggtgc cctcatcagc cacgagaagc tgctgctgca gatcaacccc
     3541 gagcgggagc tggggagcat gagctacaaa ctcggccagg tgtccataca ctccgtgtgg
     3601 ctagggaaca gcatcacacc cctgagggag gaggaatggg acgaggaaga ggaggaggag
     3661 gccgacgccc ctgcaccttc gtcaccaccc acgtctccag tcaactccag gaaacaccgg
     3721 gctggagttg acatccactc ctgttcgcag tttttgcttg agttgtacag ccgctggatc
     3781 ctgccgtcca gctcagccag gaggaccccg gccatcctga tcagtgaggt ggtcagatcc
     3841 cttctagtgg tctcagactt gttcaccgag cgcaaccagt ttgagctgat gtatgtgacg
     3901 ctgacagaac tgcgaagggt gcacccttca gaagacgaga tcctcgctca gtacctggtg
     3961 cctgccacct gcaaggcagc tgccgtcctt gggatggaca aggccgtggc ggagcctgtc
     4021 agccgcctgc tggagagcac gctcaggagc agccacctgc ccagcagggt tggagccctg
     4081 cacggcatcc tctatgtgct ggagtgcgac ctgctggacg acactgccaa gcagctcatc
     4141 ccggtcatca gcgactatct cctctccaac ctgaaaggga tcgcccactg cgtgaacatt
     4201 cacagccagc agcacgtact ggtcatgtgt gccactgcgt tttacctcat tgagaactat
     4261 cctctggacg tagggccgga attttcagca tcaataatac agatgtgtgg ggtgatgctg
     4321 tctggaagtg aggagtccac cccctccatc atttaccact gtgccctcag aggcctggag
     4381 cgcctcctgc tctctgagca gctctcccgc ctggatgcag aatcgctggt caagctgagt
     4441 gtggacagag tgaacgtgca cagcccgcac cgggccatgg cggctctggg cctgatgctc
     4501 acctgcatgt acacaggaaa ggagaaagtc agtccgggta gaacttcaga ccctaatcct
     4561 gcagcccccg acagcgagtc agtgattgtt gctatggagc gggtatctgt tctttttgat
     4621 aggatcagga aaggctttcc ttgtgaagcc agagtggtgg ccaggatcct gccccagttt
     4681 ctagacgact tcttcccacc ccaggacatc atgaacaaag tcatcggaga gtttctgtcc
     4741 aaccagcagc cataccccca gttcatggcc accgtggtgt ataaggtgtt tcagactctg
     4801 cacagcaccg ggcagtcgtc catggtccgg gactgggtca tgctgtccct ctccaacttc
     4861 acgcagaggg ccccggtcgc catggccacg tggagcctct cctgcttctt tgtcagcgcg
     4921 tccaccagcc cgtgggtcgc ggcgatcctc ccacatgtca tcagcaggat gggcaagctg
     4981 gagcaggtgg acgtgaacct tttctgcctg gtcgccacag acttctacag acaccagata
     5041 gaggaggagc tcgaccgcag ggccttccag tctgtgcttg aggtggttgc agccccagga
     5101 agcccatatc accggctgct gacttgttta cgaaatgtcg gcgggtccgg ggattacaag
     5161 gatgacgacg ataagtgaaa cctctacttc caatcgcacc atcatcacca tcaccatcac
     5221 caccatgatt acaaggatga cgacgataag tgaggcggcc gccaacttga tatcaagctt
     5281 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5341 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5401 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5461 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5521 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5581 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5641 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     5701 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     5761 gccctcaatc cagcggacct tccttcccgc gggcgccccc ctcctcacgg cgagcgaagc
     5821 ttgtcgagaa gtactagagg atctctagag cctgcagtct cgacaagctt gtcgagaagt
     5881 actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     5941 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     6001 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6061 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6121 tctggatctg atcactgctt gagcctagga gatccgaacc agataagtga aatctagttc
     6181 caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat
     6241 ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc
     6301 ccaactattt tgtccgccca cagcggggca tttttcttcc tgttatgttt ttaatcaaac
     6361 atcctgccaa ctccatgtga caaaccgtca tcttcggcta ctttttctct gtcacagaat
     6421 gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat atcaacgctt
     6481 atttgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6541 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6601 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6661 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6721 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6781 ttggagtcca cgttcttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6841 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6901 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     6961 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7021 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7081 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7141 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7201 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7261 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7321 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7381 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7441 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7501 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7561 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7621 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7681 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7741 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7801 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7861 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7921 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7981 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8041 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8101 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8161 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8221 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8281 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8341 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8401 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8461 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8521 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8581 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8641 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8701 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8761 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8821 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8881 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     8941 ccgcatagac cagccgcgta acctggcaaa atcggttacg gttgagtaat aaatggatgc
     9001 cctgcgtaag cgggtgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac
     9061 tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg
     9121 ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt
     9181 gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg catggtaaag actatattcg
     9241 cggcgttgtg acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg
     9301 aattgttagg tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc
     9361 aactttgtat agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca
     9421 taagcaccaa gcgcgttggc ctcatgcttc aagagattga tgagcgcggt ggcaatgccc
     9481 tgc
//
