LOCUS       sequence-361423-        5843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination. May not contain 
            entire coding region of gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5843)
  TITLE     SGC Structures-2021-Q3 and Q4
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UTP15:PBC028-C01:C245785
FEATURES             Location/Qualifiers
     source          1..5843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      124..578
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(211..230)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     420..441
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        604..708
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(709..777,778..1569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(927..946)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1740..2328
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2229..2248
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2482..2499
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2633..2857
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2924..3457)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3627..3632,3633..3649,3650..3655))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        4007..4098
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4023..4040
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4157..4174
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4187..4207
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    5455..5589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5492..5511)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5546..5565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5618..5783
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 aaccgtcatc ttcggctact ttttctctgt cacagaatga aaatttttct gtcatctctt
       61 cgttattaat gtttgtaatt gactgaatat caacgcttat ttgcagcctg aatggcgaat
      121 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      181 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      241 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      301 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      361 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttcttaatag
      421 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      481 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
      541 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
      601 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      661 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      721 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      781 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      841 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      901 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      961 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1021 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1081 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1141 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1201 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1261 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1321 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1381 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1441 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1501 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1561 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1621 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1681 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1741 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1801 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1861 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1921 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1981 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2041 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2101 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2161 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2221 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2281 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2341 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2401 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2461 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2521 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca gccgcgtaac
     2581 ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg
     2641 gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact
     2701 agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact
     2761 tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa
     2821 gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac aatttaccga
     2881 acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg
     2941 ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg
     3001 cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct
     3061 catgcttgag cagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga
     3121 ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag
     3181 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
     3241 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
     3301 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
     3361 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
     3421 tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     3481 gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca
     3541 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     3601 gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg
     3661 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
     3721 tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc
     3781 aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgccctggc
     3841 ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg
     3901 aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc caggactcta
     3961 gctatagttc tagtggttgg ctacgtatac tccggaatat taatagatca tggagataat
     4021 taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa cagttttgta
     4081 ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg gcgcggatct
     4141 cggtccgaaa accatgcatc atcatcatca tcacagcagc ggcagagaaa acttgtattt
     4201 ccagggcatg gctggttata agcctgtagc tattcagaca tatcctatac ttggtgaaaa
     4261 aatcacccaa gatacactgt actggaacaa ctataagacc cctgttcaga ttaaggaatt
     4321 tggtgcagtt tcaaaagtag acttttctcc tcagcctcca tataattatg ctgtcacagc
     4381 ttcctcaaga attcacattt atggccgata ctcccaagaa cctataaaaa ccttttctcg
     4441 atttaaagac acagcatact gtgctacttt tcgacaagat ggtagattgc ttgtggctgg
     4501 cagtgaagat ggtggagttc aactttttga tataagtggg agggctcccc tcaggcagtt
     4561 tgaaggccat acaaaagcag ttcatacagt agattttaca gctgacaaat atcacgtggt
     4621 ctctggggct gatgattata cagttaaatt atgggatatt ccaaactcca aagaaatttt
     4681 gacatttaaa gaacactctg attatgtgag gtgtggatgt gctagcaaac ttaatccgga
     4741 tctctttata acaggatcat atgatcatac tgtgaagatg tttgatgcac gaacgagtga
     4801 gagtgttctc tccgttgagc atgggcagcc agtggagagt gtcctacttt tcccctctgg
     4861 aggtcttctg gtgtcagcag gaggtcgtta tgttaaagtc tgggacatgt taaaaggagg
     4921 acaattgcta gtatctttga aaaatcatca caaaaccgtg acatgtttat gtctaagcag
     4981 ctctggacag aggttactct ctggctcact ggataggaag gtgaaagtat acagcacaac
     5041 ttcctacaaa gtagtccaca gttttgatta tgcagcttca attttgagtc ttgcccttgc
     5101 acatgaagat gagacaatag ttgtaggaat gaccaatgga atactgagtg ttaaacatcg
     5161 gaaatctgaa gcaaagaagg aatcacttcc cagaagaaga aggcctgcat atcgaacctt
     5221 tattaaagga aaaaattaca tgaagcaacg ggatgacatt ttgattaaca ggccagcaaa
     5281 gaagcaccta gaattgtatg acagggatct gaaacattga tgacgaagct tgtcgagaag
     5341 tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     5401 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     5461 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5521 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5581 gtctggatct gatcactgat atcgcctagg agatccgaac cagataagtg aaatctagtt
     5641 ccaaactatt ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca
     5701 tctattttgt cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt
     5761 cccaactatt ttgtccgccc acagcggggc atttttcttc ctgttatgtt tttaatcaaa
     5821 catcctgcca actccatgtg aca
//
