LOCUS       sequence-361427-        5675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination. May not contain 
            entire coding region of gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5675)
  TITLE     SGC Structures-2021-Q3 and Q4
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DCAF1:PBC020-D05:C246337
FEATURES             Location/Qualifiers
     source          1..5675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     28..45
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  179..403
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(470..1003)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1173..1178,1179..1195,1196..1201))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1553..1644
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1569..1586
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1703..1720
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1733..1753
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2833..2967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2870..2889)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2924..2943
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2996..3161
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3345..3799
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3432..3451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3641..3662
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3825..3929
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3930..3998,3999..4790)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4148..4167)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4961..5549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5450..5469
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
       61 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat agaccagccg
      121 cgtaacctgg caaaatcggt tacggttgag taataaatgg atgccctgcg taagcgggtg
      181 tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac aataaagtct
      241 taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga aaaagcatac
      301 tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat tgcccgtcgt
      361 attaaagagg ggcgtggcca agggcatggt aaagactata ttcgcggcgt tgtgacaatt
      421 taccgaacaa ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg
      481 tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag
      541 ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt
      601 tggcctcatg cttgagcaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc
      661 cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa
      721 cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg
      781 tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg
      841 ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt
      901 cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag
      961 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
     1021 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac
     1081 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc
     1141 agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca
     1201 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca
     1261 gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc
     1321 atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc
     1381 cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc
     1441 cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg
     1501 actctagcta tagttctagt ggttggctac gtatactccg gaatattaat agatcatgga
     1561 gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt
     1621 tttgtaataa aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc
     1681 ggatctcggt ccgaaaacca tgcatcatca tcatcatcac agcagcggca gagaaaactt
     1741 gtatttccag ggcgctagcg cattccgtcc tatttcagtg ttccgggaag ccaatgaaga
     1801 tgagagtggc ttcacctgct gtgcattctc agcacgggag cggttcctga tgcttggcac
     1861 ctgcacaggg cagctgaagc tctataatgt gtttagtgga caggaggagg ccagctataa
     1921 ctgtcacaac tcagccatca cacatcttga accttccagg gatgggtcct tgctgctgac
     1981 atctgctact tggagccagc ctttgtctgc actttgggga atgaagtcag tatttgatat
     2041 gaagcattcc ttcacagaag atcactatgt tgagttcagt aagcactccc aggatcgggt
     2101 catcggcaca aaaggagaca ttgcccacat ttatgatatt cagactggca acaagctgtt
     2161 gactctgttt aacccagatc ttgccaacaa ctacaagagg aactgtgcca cctttaatcc
     2221 tacagatgat cttgtcttaa atgatggcgt cctctgggat gtccgctctg cacaggccat
     2281 ccacaagttt gacaagttca atatgaacat cagtggtgtt ttccatccaa atggactgga
     2341 ggtgatcatt aatactgaga tttgggacct tcgaactttt catcttttgc atactgttcc
     2401 cgctctggat cagtgtcgcg tggtgttcaa tcacacggga acagtgatgt atggagctat
     2461 gttgcaggca gatgatgaag atgacttaat ggaagagagg atgaaaagcc cctttgggtc
     2521 atccttccga acatttaatg caactgacta caaacctata gcaaccattg atgtgaaacg
     2581 gaacatcttt gacctgtgta cagacaccaa agactgctat cttgctgtca ttgagaatca
     2641 aggcagcatg gatgccctga acatggacac agtatgcagg ctgtatgaag tgggctgatg
     2701 acgaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt
     2761 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     2821 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     2881 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     2941 atcaatgtat cttatcatgt ctggatctga tcactgatat cgcctaggag atccgaacca
     3001 gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga
     3061 cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca
     3121 tttccacccc tcccagttcc caactatttt gtccgcccac agcggggcat ttttcttcct
     3181 gttatgtttt taatcaaaca tcctgccaac tccatgtgac aaaccgtcat cttcggctac
     3241 tttttctctg tcacagaatg aaaatttttc tgtcatctct tcgttattaa tgtttgtaat
     3301 tgactgaata tcaacgctta tttgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     3361 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3421 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3481 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3541 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3601 ggtttttcgc cctttgacgt tggagtccac gttcttaata gtggactctt gttccaaact
     3661 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     3721 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     3781 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3841 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3901 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3961 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4021 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4081 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4141 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4201 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4261 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4321 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4381 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4441 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4501 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4561 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4621 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4681 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4741 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4801 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     4861 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4921 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4981 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5041 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5101 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5161 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5221 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5281 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5341 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5401 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5461 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5521 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5581 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5641 ataaccgtat taccgccttt gagtgagctg atacc
//
