LOCUS       sequence-361428-        9508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tdTomato with fragment nucleotides 441-676 of beta-actin 3'UTR 
            inserted in 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Dasgupta S, Dayagi DY, Haimovich G, Wyler E, Olender T, Singer RH, 
            Landthaler M, Gerst JE
  TITLE     Global analysis of contact-dependent human-to-mouse intercellular 
            mRNA and lncRNA transfer in cell culture.
  JOURNAL   Elife. 2023 May 30;12:e83584. doi: 10.7554/eLife.83584.
  PUBMED    37249209
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-05-30"; volume: "12:e83584. doi"; pages: " 
            10.7554/eLife.83584"
COMMENT     Sequence Label: tdTomato-beta-actin3'UTR2
FEATURES             Location/Qualifiers
     source          1..9508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..110
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(111..179,180..971)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(329..348)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1142..1730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1631..1650
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1884..1901
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2018..2039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2054..2059,2060..2077,2078..2084)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2092..2108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2097..2119
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2116..2132
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2116..2132
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2151..2171
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2153..2171
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2272..2651
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2652..2855
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2806..2826
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2870..3050
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3097..3222
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3715..3948
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4133..4177
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4444..4561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4618..5829
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5666..5685
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5863..5872
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5869..7299
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    7551..8138
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7603..7623)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8021..8032)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8225..8458
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8530..8651
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8567..8586)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8621..8640
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8691..8826
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8846..8865)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8847..8865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8875..8892)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8875..8891)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8884..8906)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9033..9488
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9120..9139)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9330..9351
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
       61 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      121 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      181 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      241 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      301 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      361 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
      421 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
      481 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
      541 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
      601 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
      661 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
      721 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
      781 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
      841 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
      901 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
      961 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1021 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1081 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1141 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1201 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1261 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1321 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1381 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1441 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1501 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1561 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1621 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1681 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1741 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1801 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     1861 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     1921 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1981 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     2041 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     2101 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     2161 ctcactaaag ggaacaaaag ctggagctgc aagcttggcc attgcatacg ttgtatccat
     2221 atcataatat gtacatttat attggctcat gtccaacatt accgccatgt tgacattgat
     2281 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     2341 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     2401 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     2461 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     2521 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     2581 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     2641 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     2701 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     2761 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     2821 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgg ggtctctctg
     2881 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     2941 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     3001 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     3061 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     3121 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     3181 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     3241 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     3301 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     3361 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     3421 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     3481 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     3541 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     3601 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     3661 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     3721 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     3781 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     3841 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     3901 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     3961 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     4021 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     4081 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     4141 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     4201 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     4261 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     4321 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     4381 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     4441 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     4501 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     4561 tatcgatcac gagactagcc tcgagaagct tgattaaccc gtgtcggctc cagatctggc
     4621 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     4681 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     4741 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     4801 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     4861 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     4921 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     4981 ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc
     5041 cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa
     5101 gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag
     5161 caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt
     5221 cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc
     5281 taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac
     5341 gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat
     5401 ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg
     5461 acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg
     5521 taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga
     5581 caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt
     5641 ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg
     5701 gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata
     5761 tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt
     5821 ttgttagacg aagcttgggc tgcaggtcga gatccaccgg tcgccaccat ggtgagcaag
     5881 ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
     5941 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     6001 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     6061 cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag
     6121 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt
     6181 ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     6241 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     6301 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
     6361 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
     6421 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
     6481 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     6541 ttcctggggc atggcaccgg cagcaccggc agcggcagct ccggcaccgc ctcctccgag
     6601 gacaacaaca tggccgtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
     6661 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     6721 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     6781 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
     6841 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     6901 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     6961 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     7021 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     7081 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     7141 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     7201 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     7261 cacctgttcc tgtacggcat ggacgagctg tacaagtagc attgcttctg tgtaaattat
     7321 gtactgcaaa aattttttta aatcttccgc cttaatactt catttttgtt tttaatttct
     7381 gaatggccca ggtctgaggc ctcccttttt tttgtccccc caacttgatg tatgaaggct
     7441 ttggtctccc tgggaggggg ttgaggtgtt gaggcagcca gggctggcct gtacactgac
     7501 ttgagaccaa taaaagtgca caccttacct tacacagcgg ccgcgtcgac aatcaacctc
     7561 tggattaaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7621 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7681 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7741 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7801 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7861 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7921 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     7981 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     8041 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     8101 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacct
     8161 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     8221 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     8281 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8341 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8401 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8461 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     8521 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8581 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8641 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     8701 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     8761 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8821 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     8881 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     8941 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     9001 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     9061 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     9121 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     9181 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     9241 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     9301 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     9361 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     9421 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     9481 tacaatttag gtggcacttt tcggggaa
//
