LOCUS       sequence-361431-        9280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tdTomato expression from UbiC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Dasgupta S, Dayagi DY, Haimovich G, Wyler E, Olender T, Singer RH, 
            Landthaler M, Gerst JE
  TITLE     Global analysis of contact-dependent human-to-mouse intercellular 
            mRNA and lncRNA transfer in cell culture.
  JOURNAL   Elife. 2023 May 30;12:e83584. doi: 10.7554/eLife.83584.
  PUBMED    37249209
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-05-30"; volume: "12:e83584. doi"; pages: " 
            10.7554/eLife.83584"
COMMENT     Sequence Label: pUbC-tdTomato
FEATURES             Location/Qualifiers
     source          1..9280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        46..150
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(151..219,220..1011)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(369..388)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1182..1770
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1671..1690
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1924..1941
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2058..2079
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2094..2099,2100..2117,2118..2124)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2132..2148
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2137..2159
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2156..2172
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2156..2172
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2191..2211
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2193..2211
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2312..2691
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2692..2895
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2846..2866
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2910..3090
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3137..3262
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3755..3988
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4173..4217
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4484..4601
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4658..5869
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5706..5725
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5903..5912
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5909..7339
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    7362..7950
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7415..7435)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7833..7844)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8037..8270
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8342..8463
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8379..8398)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8433..8452
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8503..8638
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8658..8677)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8659..8677)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8687..8704)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8687..8703)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8696..8718)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8845..9273
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8932..8951)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9142..9163
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
       61 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      121 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      181 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      241 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      301 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      361 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
      421 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      481 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
      541 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
      601 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
      661 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
      721 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
      781 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
      841 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
      901 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
      961 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1021 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1081 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1141 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1201 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1261 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1321 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1381 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1441 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1501 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1561 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1621 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1681 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1741 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1801 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1861 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1921 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     1981 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2041 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     2101 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     2161 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     2221 ctggagctgc aagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat
     2281 attggctcat gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag
     2341 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     2401 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     2461 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     2521 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     2581 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     2641 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     2701 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     2761 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     2821 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     2881 tatataagca gagctcgttt agtgaaccgg ggtctctctg gttagaccag atctgagcct
     2941 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     3001 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     3061 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     3121 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     3181 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     3241 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     3301 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     3361 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     3421 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     3481 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     3541 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     3601 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     3661 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     3721 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     3781 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     3841 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     3901 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     3961 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     4021 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     4081 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     4141 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     4201 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     4261 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     4321 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     4381 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     4441 gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg
     4501 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     4561 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac gagactagcc
     4621 tcgagaagct tgattaaccc gtgtcggctc cagatctggc ctccgcgccg ggttttggcg
     4681 cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc
     4741 gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg
     4801 gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg
     4861 gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat
     4921 tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt
     4981 gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc
     5041 tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg
     5101 ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc
     5161 gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg
     5221 agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg
     5281 catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat
     5341 tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg
     5401 gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg
     5461 cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc
     5521 ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag
     5581 gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg
     5641 aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa
     5701 gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt
     5761 tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac
     5821 tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttgggc
     5881 tgcaggtcga gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg tcatcaaaga
     5941 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     6001 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     6061 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     6121 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     6181 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     6241 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     6301 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     6361 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     6421 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     6481 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     6541 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg
     6601 cagcaccggc agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat
     6661 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
     6721 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     6781 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
     6841 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
     6901 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
     6961 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
     7021 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     7081 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
     7141 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
     7201 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
     7261 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat
     7321 ggacgagctg tacaagtagg gatccgagcg gccgcgtcga caatcaacct ctggattaca
     7381 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7441 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7501 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7561 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7621 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7681 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7741 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     7801 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7861 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7921 gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc
     7981 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     8041 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     8101 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8161 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8221 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8281 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8341 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8401 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8461 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     8521 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8581 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8641 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     8701 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     8761 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8821 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     8881 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     8941 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     9001 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     9061 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     9121 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     9181 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     9241 atgagctgat ttaacaaaaa tttaacgcga attttaacaa
//
