LOCUS       sequence-361439-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TC6
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(108..127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1024..1040
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1024..1040
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1045..1064
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1045..1063
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1238..2428)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2057..2076
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2476..2481,2482..2498,2499..2504))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
       61 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      661 cgacgcgtaa ctcacgttaa gggattttgg tcatgggtgg ctcgaccaga tatggtctca
      721 ggctatcgct acaacttgcg ctggggttag tccgtgggct tgacagagaa ctggtagaag
      781 gcgttggacg gtttcaagag cctgtttgcc accgtcatga ctagcaatcg ctacaacttg
      841 ctcgggcgtc aggccgtggg cttgacagag aactggtaga aggcgctgaa cagtctcaag
      901 agcctgtttg ccacccccat tactagcaat ccgagaccag ctttggtcta gaggaggcat
      961 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac gggccagagc
     1021 tgccaggaaa cagctatgac catgtaatac gactcactat aggggatatt ttattttctc
     1081 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
     1141 caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga
     1201 gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc
     1261 atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg
     1321 ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa
     1381 tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt
     1441 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga
     1501 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc
     1561 ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
     1621 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca
     1681 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
     1741 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
     1801 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatgggtc
     1861 ggcgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     1921 tgagcaccgc cgccgcaagg aatggtgcat gtaaggagat ggcgcccaac agtcccccgg
     1981 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     2041 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     2101 cggtgatgcc ggccacgatg cgtccggcgt agagaatcca caggacgggt gtggtcgcca
     2161 tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa
     2221 agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg
     2281 ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc
     2341 ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga
     2401 tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact
     2461 gtgataaact accgcattaa agctaatcga tgataagctg tcaaacatga gaattaattc
     2521 atgttctttc ctgcgttatc ccctgattct gtggataacg caggaaagaa catgtgagca
     2581 aaaggccagc aaaaggccag gaa
//
