LOCUS       sequence-361440-       11901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human PEX6 fused to Tomato, and PEX6 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11901)
  AUTHORS   Haimovich G, Dasgupta S, Ravi AG, Gerst JE
  TITLE     Complementation of a human disease phenotype in vitro by 
            intercellular mRNA transfer
  JOURNAL   bioRxiv 2024.11.06.622258
REFERENCE   2  (bases 1 to 11901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.06.622258"
COMMENT     Sequence Label: pHAGE-PEX6-Tomato
FEATURES             Location/Qualifiers
     source          1..11901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..106
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(107..175,176..967)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(325..344)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1138..1726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1627..1646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1880..1897
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2014..2035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2050..2055,2056..2073,2074..2080)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2088..2104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2093..2115
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2112..2128
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2112..2128
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2147..2167
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2149..2167
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2268..2647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2648..2851
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2802..2822
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2866..3046
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3093..3218
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3711..3944
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4129..4173
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4440..4557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4614..5825
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5662..5681
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5859..5868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8805..9503
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(8935..8953)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9203..9222
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9397..9420
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9939..10527
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9992..10012)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10410..10421)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10614..10847
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10919..11040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10956..10975)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11010..11029
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11080..11215
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11235..11254)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11236..11254)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11264..11281)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11264..11280)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11273..11295)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11422..11877
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11509..11528)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11719..11740
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
       61 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      121 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      181 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      241 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      301 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      361 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      421 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      481 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
      541 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
      601 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
      661 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
      721 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
      781 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
      841 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
      901 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
      961 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1021 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1081 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1141 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1201 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1261 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1321 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1381 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1441 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1501 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     1561 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1621 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     1681 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     1741 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     1801 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     1861 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     1921 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     1981 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     2041 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     2101 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     2161 ctaaagggaa caaaagctgg agctgcaagc ttggccattg catacgttgt atccatatca
     2221 taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac attgattatt
     2281 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     2341 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     2401 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     2461 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     2521 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2581 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2641 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2701 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2761 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     2821 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccggggtc tctctggtta
     2881 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2941 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     3001 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     3061 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     3121 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     3181 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     3241 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     3301 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     3361 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3421 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3481 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3541 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3601 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3661 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3721 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3781 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3841 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3901 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3961 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     4021 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     4081 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     4141 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     4201 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     4261 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     4321 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     4381 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4441 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4501 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc
     4561 gatcacgaga ctagcctcga gaagcttgat taacccgtgt cggctccaga tctggcctcc
     4621 gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc
     4681 agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg
     4741 ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga
     4801 cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta
     4861 gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata
     4921 taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt
     4981 cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg
     5041 gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc
     5101 tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa
     5161 atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt
     5221 gaaacaaggt ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat
     5281 gcgggaaagc tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga
     5341 agtttgtcac tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg
     5401 gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt
     5461 cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg
     5521 cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg
     5581 cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat
     5641 gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga
     5701 gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta
     5761 attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt
     5821 tagacgaagc ttgggctgca ggtcgagatc caccggtcgc caccatggcg ctggctgtct
     5881 tgcgggtcct ggagcccttt ccgaccgaga cacccccgtt ggcagtgctg ctgccacccg
     5941 ggggcccgtg gccggcggcg gagctgggcc tggtgctggc cctgaggcct gcaggggaga
     6001 gcccggcagg gccggcgctg ctggtggcag ccctggaggg gccggacgcg ggcaccgaag
     6061 agcagggtcc cgggccgccg cagctactgg ttagccgcgc gctgctgcgg ctcctggcac
     6121 tgggctccgg ggcctgggtg cgggcgcggg cggtgcggcg gcccccggcg ctaggttggg
     6181 cactgcttgg cacctcgctg gggcctgggc tcggaccgcg agtcgggccg ctgctggtga
     6241 ggcgcggaga gaccctccca gttcccggac cgcgggtgct ggagacgcgg ccggcgttgc
     6301 aagggctgct gggcccaggg actcggctgg ctgtgactga gctccgcggg cgggccagac
     6361 tgtgtccaga gtctggggac agcagtcggc ccccaccccc gcccgtggtg tcctcctttg
     6421 cggtttctgg cacagtgcgg cgactccagg gagttctggg agggactgga gattcactag
     6481 gggtgagccg gagctgtctc cgtggccttg gcctcttcca gggcgaatgg gtgtgggtgg
     6541 cccaggccag agagtcatcg aacacttcac agccgcactt ggctagggtg caggtcctag
     6601 aacctcgctg ggacctctct gatagactgg gacccggctc tggaccgctg ggagagcccc
     6661 tcgctgacgg actggcgctt gtccctgcca ctttggcttt taatcttggc tgtgaccccc
     6721 tggaaatggg agagctcaga attcagaggt acttggaagg ctccatcgcc cctgaagaca
     6781 aaggaagctg ctcattgctg cctgggcctc catttgccag agagttacac atcgaaattg
     6841 tgtcttctcc ccactacagc actaatggaa attatgacgg tgttctttac cggcactttc
     6901 agatacccag ggtagtccag gaaggggatg ttctatgtgt gccaacaatt gggcaagtag
     6961 agatcctgga aggaagtcca gagaaactgc ccaggtggcg ggaaatgttt tttaaagtga
     7021 agaaaacagt tggggaagct ccagatggac cagccagtgc ctacttggcc gacaccaccc
     7081 atacctcctt gtacatggtg ggttctaccc tgagccctgt tccatggctc ccttcagagg
     7141 aatccactct ctggagcagt ttgtctcctc caggcctgga ggccttggtg tctgaactct
     7201 gtgctgtcct gaagcctcgc ctccagccag ggggtgccct gctgacagga actagcagtg
     7261 tccttctacg gggcccccca ggctgtggga agaccacagt agttgctgct gcctgtagtc
     7321 accttgggct ccacttactg aaggtgccct gctccagcct ctgtgcagaa agtagtgggg
     7381 ctgtggagac aaaactgcag gccatcttct cccgggcccg ccgttgccgg cctgcagtcc
     7441 tgttgctcac agctgtggac cttctgggcc gggaccgtga tgggctgggt gaggatgccc
     7501 gtgtgatggc tgtgctgcgt cacctcctcc tcaatgagga ccccctcaac agctgccctc
     7561 ccctcatggt tgtggccacc acaagccggg cccaggacct gcctgctgat gtgcagacag
     7621 catttcctca tgagctcgag gtgcctgctc tgtcagaggg gcagcggctc agcatcctgc
     7681 gggccctcac tgcccacctt cccctgggcc aggaggtgaa cttggcacag ctagcacggc
     7741 ggtgtgcagg ctttgtggta ggggatctct atgcccttct gacccacagc agccgggcag
     7801 cctgcaccag gatcaagaac tcaggtttgg caggtggctt gactgaggag gatgaggggg
     7861 agctgtgtgc tgccggcttt cctctcctgg ctgaggactt tgggcaggca ctggagcaac
     7921 tgcagacagc tcactcccag gccgttggag cccccaagat cccctcagtg tcctggcatg
     7981 atgtgggtgg gctgcaggag gtgaagaagg agatcctgga gaccattcag ctccccctgg
     8041 agcaccctga gctactgagc ctgggcctga gacgctcagg ccttctgctc catgggcccc
     8101 ctggcaccgg caagaccctt ctggccaagg cagtagccac tgagtgcagc cttaccttcc
     8161 tcagcgtgaa ggggccagag ctcattaaca tgtatgtggg ccaaagtgag gagaatgtgc
     8221 gggaagtgtt tgccagggcc agggctgcag ctccatgcat tatcttcttt gatgaactgg
     8281 actctttggc cccaagccgg gggcgaagtg gagattctgg aggagtgatg gacagggtgg
     8341 tgtctcagct ccttgccgag ctagatgggc tgcacagcac tcaggatgtg tttgtgattg
     8401 gagccaccaa cagaccagat ctcctggacc ctgcccttct gcggcctggc agatttgaca
     8461 agctggtgtt tgtggaggca aatgaggacc gggcctccca gctacgcgtt ctaagtgcca
     8521 tcacacgcaa attcaagcta gagccatctg tgagcctggt aaacgtgcta gattgctgcc
     8581 ctccccagct gacgggcgcg gacctctact ctctctgctc tgatgctatg acagctgccc
     8641 tcaaacgcag ggttcatgac ctggaggaag ggctggaacc aggtagctca gcactgatgc
     8701 tcaccatgga ggacttgctg caggctgccg cccggctgca accctcagtc agtgagcagg
     8761 agctgctccg gtacaagcgc atccagcgca agtttgctgc ctgcgtgagc aagggcgagg
     8821 aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg
     8881 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     8941 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca
     9001 tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt
     9061 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga
     9121 ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg
     9181 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     9241 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga
     9301 agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc
     9361 ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg
     9421 aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt
     9481 acggcatgga cgagctgtac aagtaagagc cccccagggt ctgggacccc gctcagcatg
     9541 gctgcaggta ccttgatagc ccacagagag atctgggaag gaagggctcc tcctcaggct
     9601 gctgccaacc cacctggagg ccacctccct ccaggagatc ccagggtgca aagtggcatt
     9661 gagacagcag caacagctca agagatatct cctgcctact tgcccctcct tccaggccgg
     9721 ctctaagaga aaggcccatc tactcaggaa gagggccagg gccttgggtt ctggggattg
     9781 ggccctgaga gggctagttc tgtggctgaa aataaagcat gtcccgcccc ctactggtgt
     9841 tgtggcatga aaggttggag tgagaaagag cagggttgtg ggaggagtaa gccctgggag
     9901 agggtggggg ggtgggcagc atgagcggcc gcgtcgacaa tcaacctctg gattacaaaa
     9961 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
    10021 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
    10081 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
    10141 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
    10201 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
    10261 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
    10321 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
    10381 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
    10441 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
    10501 ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat
    10561 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
    10621 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
    10681 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    10741 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    10801 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
    10861 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
    10921 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    10981 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    11041 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
    11101 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
    11161 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
    11221 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
    11281 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    11341 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    11401 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
    11461 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
    11521 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
    11581 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
    11641 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
    11701 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
    11761 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
    11821 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
    11881 tggcactttt cggggaaatg t
//
