LOCUS       sequence-361441-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TC5
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     646..662
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     646..662
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     667..686
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        667..685
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(860..2050)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1679..1698
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2098..2103,2104..2120,2121..2126))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2253..2602,1..239))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2333..2352)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
       61 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
      121 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
      181 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
      241 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgacgcg taactcacgt
      301 taagggattt tggtcatggg tggctcgacc agatatggtc tcaaatcgct acaacttgcg
      361 ctggggttag tccgtgggct tgacagagaa ctggtagaag gcgttggacg gtttcaagag
      421 cctgtttgcc accgtcatga ctagcaatcg ctacaacttg ctcgggcgtc aggccgtggg
      481 cttgacagag aactggtaga aggcgctgaa cagtctcaag agcctgtttg ccacccccat
      541 tactagcaat tgccgagacc agctttggtc tagaggaggc atgatgatat atttttatct
      601 tgtgcaatgt aacatcagag attttgagac acgggccaga gctgccagga aacagctatg
      661 accatgtaat acgactcact ataggggata ttttattttc tcccaatcag gcttgatccc
      721 cagtaagtca aaaaatagct cgacatactg ttcttccccg atcaagggtt ggtttgcgca
      781 ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg
      841 aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg
      901 cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc
      961 tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc aatgatcgaa gttaggctgg
     1021 taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca
     1081 gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga
     1141 aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca
     1201 tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg
     1261 cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc
     1321 tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga
     1381 gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc
     1441 ggaaggagct gactgggttg aaggctctca agggcatggg tcggcgctct cccttatgyg
     1501 actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa
     1561 ggaatggtgc atgtaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
     1621 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     1681 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     1741 tgcgtccggc gtagagaatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
     1801 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc
     1861 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt
     1921 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa
     1981 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt
     2041 tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt
     2101 aaagctaatc gatgataagc tgtcaaacat gagaattaat tcatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2221 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2281 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2341 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2401 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2461 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2521 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2581 cacgacttat cgccactggc ag
//
