LOCUS       sequence-361449-        2003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 4 reverse promoter part for golden gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Leonard SP, Powell JE, Perutka J, Geng P, Heckmann LC, Horak RD, 
            Davies BW, Ellington AD, Barrick JE, Moran NA
  TITLE     Engineered symbionts activate honey bee immunity and limit 
            pathogens.
  JOURNAL   Science. 2020 Jan 31;367(6477):573-576. doi: 
            10.1126/science.aax9039. Epub 2020 Jan 30.
  PUBMED    32001655
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aax9039"; journalName: "Science"; date: 
            "2020-01-31- 31"; volume: "367"; issue: "6477"; pages: "573-576"
COMMENT     Sequence Label: pBTK151
FEATURES             Location/Qualifiers
     source          1..2003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(172..206)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(277..936)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     851..870
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1118..1706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1607..1626
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1939..1987)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 cggtctcaat ccaacagtac tatgtgatta taccagcccc ctcactacat gtcaagaata
       61 aactgccaaa ggtaatcgtt aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt
      121 ttatgggggg agtttaggga aagagcattt gtcagctgtg agaccagacc aataaaaaac
      181 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
      241 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
      301 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
      361 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
      421 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
      481 actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata
      541 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
      601 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
      661 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
      721 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
      781 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
      841 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
      901 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
      961 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     1021 acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact
     1081 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1141 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1201 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1261 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1321 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1381 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1441 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1501 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1561 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1621 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1681 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1741 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1801 accgtagcgg gagagtgttc accgacaaac aacagataaa acaaaaggcc cagtcttccg
     1861 actgagcctt ttgttttatt tgatgtctgg cagttccccg ccgctatctg atcatcaaat
     1921 aataatttct tcatgttgaa aatctccaaa aaaaaaggct ccaaaaggag cctttaattg
     1981 tatcggttta tcagcttgct ttt
//
