LOCUS       sequence-361457-       10266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway expression vector encoding D1424H mutant MYH9 cDNA, with 
            N-terminal Flag tag. For mammalian expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Panza E, Marini M, Pecci A, Giacopelli F, Bozzi V, Seri M, Balduini 
            C, Ravazzolo R
  TITLE     Transfection of the mutant MYH9 cDNA reproduces the most typical 
            cellular phenotype of MYH9-related disease in different cell lines.
  JOURNAL   Pathogenetics. 2008 Dec 1;1(1):5. doi: 10.1186/1755-8417-1-5.
  PUBMED    19046415
REFERENCE   2  (bases 1 to 10266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Pathogenetics."; date: "2008-12-1"; volume: "1(1):5. doi"; pages: "
            10.1186/1755-8417-1-5"
COMMENT     Sequence Label: pMYH9-D1424H-FLAG
FEATURES             Location/Qualifiers
     source          1..10266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      673..682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             682..705
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    745..769
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6656..6680)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6695..6711)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6696..6712)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6767..6786)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6768..6786)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    7060..7181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7097..7116)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7151..7170
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7188..7643)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7325..7346)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7537..7556
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7670..7774
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7776..8133
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7776..7967
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7799..7819)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7984..8119
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8168..8962
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8168..8962
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8222..8241)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8832..8851
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9150..9169)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9194..9241
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9570..10158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10059..10078
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgccaccat ggattacaag gatgacgacg ataagagccc gggcggatcc
      721 cccgggctgc aggaattcga tatcacaagt ttgtacaaaa aagcaggctc catggcacag
      781 caagctgccg ataagtatct ctatgtggat aaaaacttca tcaacaatcc gctggcccag
      841 gccgactggg ctgccaagaa gctggtatgg gtgccttccg acaagagtgg ctttgagcca
      901 gccagcctca aggaggaggt gggcgaagag gccatcgtgg agctggtgga gaatgggaag
      961 aaggtgaagg tgaacaagga tgacatccag aagatgaacc cgcccaagtt ctccaaggtg
     1021 gaggacatgg cagagctcac gtgcctcaac gaagcctcgg tgctgcacaa cctcaaggag
     1081 cgttactact cagggctcat ctacacctat tcaggcctgt tctgtgtggt catcaatcct
     1141 tacaagaacc tgcccatcta ctctgaagag attgtggaaa tgtacaaggg caagaagagg
     1201 cacgagatgc cccctcacat ctatgccatc acagacaccg cctacaggag tatgatgcaa
     1261 gaccgagaag atcaatccat cttgtgcact ggtgaatctg gagctggcaa gacggagaac
     1321 accaagaagg tcatccagta tctggcgtac gtggcgtcct cgcacaagag caagaaggac
     1381 cagggcgagc tggagcggca gctgctgcag gccaacccca tcctggaggc cttcgggaac
     1441 gccaagaccg tgaagaatga caactcctcc cgcttcggca aattcattcg catcaacttt
     1501 gatgtcaatg gctacattgt tggagccaac attgagactt atcttttgga gaaatctcgt
     1561 gctatccgcc aagccaagga agaacggacc ttccacatct tctattatct cctgtctggg
     1621 gctggagagc acctgaagac cgatctcctg ttggagccgt acaacaaata ccgcttcctg
     1681 tccaatggac acgtcaccat ccccgggcag caggacaagg acatgttcca ggagaccatg
     1741 gaggccatga ggattatggg catcccagaa gaggagcaaa tgggcctgct gcgggtcatc
     1801 tcaggggttc ttcagctcgg caacatcgtc ttcaagaagg agcggaacac tgaccaggcg
     1861 tccatgcccg acaacacagc tgcccaaaag gtgtcccatc tcttgggtat caatgtgacc
     1921 gatttcacca gaggaatcct caccccgcgc atcaaggtgg gacgggatta cgtccagaag
     1981 gcgcagacta aagagcaggc tgactttgcc atcgaggcct tggccaaggc gacctatgag
     2041 cggatgttcc gctggctggt gctgcgcatc aacaaggctc tggacaagac caagaggcag
     2101 ggcgcctcct tcatcgggat cctggacatt gccggcttcg agatctttga tctgaactcg
     2161 tttgagcagc tgtgcatcaa ttacaccaat gagaagctgc agcagctctt caaccacacc
     2221 atgttcatcc tggagcagga ggagtaccag cgcgagggca tcgagtggaa cttcatcgac
     2281 tttggcctcg acctgcagcc ctgcatcgac ctcattgaga agccagcagg ccccccgggc
     2341 attctggccc tgctggacga ggagtgctgg ttccccaaag ccaccgacaa gagcttcgtg
     2401 gagaaggtga tgcaggagca gggcacccac cccaagttcc agaagcccaa gcagctgaag
     2461 gacaaagctg atttctgcat tatccactat gccggcaagg tggattacaa agctgacgag
     2521 tggctgatga agaacatgga tcccctgaat gacaacatcg ccacactgct ccaccagtcc
     2581 tctgacaagt ttgtctcgga gctgtggaag gatgtggacc gcatcatcgg cctggaccag
     2641 gtggccggca tgtcggagac cgcactgccc ggggccttca agacgcggaa gggcatgttc
     2701 cgcactgtgg ggcagcttta caaggagcag ctggccaagc tgatggctac gctgaggaac
     2761 acgaacccca actttgtccg ctgcatcatc cccaaccacg agaagaaggc cggcaagctg
     2821 gacccgcatc tcgtgctgga ccagctgcgc tgcaacggtg ttctcgaggg catccgtatc
     2881 tgccgccagg gcttccccaa cagggtggtc ttccaggagt ttcggcagag atatgagatc
     2941 ctgactccaa actccattcc caagggtttc atggacggga agcaggcgtg cgtgctcatg
     3001 ataaaagccc tggagctcga cagcaatctg taccgcattg gccagagcaa agtcttcttc
     3061 cgtgccggtg tgctggccca cctggaggag gagcgagacc tgaagatcac cgacgtcatc
     3121 atagggttcc aggcctgctg caggggctac ctggccagga aagcatttgc caagcggcag
     3181 cagcagctta ccgccatgaa ggtcctccag cggaactgcg ctgcctacct gaagctgcgg
     3241 aactggcagt ggtggcggct cttcaccaag gtcaagccgc tgctgcaggt gagccggcag
     3301 gaggaggaga tgatggccaa ggaggaggag ctggtgaagg tcagagagaa gcagctggct
     3361 gcggagaaca ggctcacgga gatggagacg ctgcagtctc agctcatggc agagaaattg
     3421 cagctgcagg agcagctcca ggcagaaacc gagctgtgtg ccgaggctga ggagctccgg
     3481 gcccgcctga ccgccaagaa gcaggaatta gaagagatct gccatgacct agaggccagg
     3541 gtggaggagg aggaggagcg ctgccagcac ctgcaggcgg agaagaagaa gatgcagcag
     3601 aacatccagg agcttgagga gcagctggag gaggaggaga gcgcccggca gaagctgcag
     3661 ctggagaagg tgaccaccga ggcgaagctg aaaaagctgg aggaggagca gatcatcctg
     3721 gaggaccaga actgcaagct ggccaaggaa aagaaactgc tggaagacag aatagctgag
     3781 ttcaccacca acctcacaga agaggaggag aaatctaaga gcctcgccaa gctcaagaac
     3841 aagcatgagg caatgatcac tgacttggaa gagcgcctcc gcagggagga gaagcagcga
     3901 caggagctgg agaagacccg ccggaagctg gagggagact ccacagacct cagcgaccag
     3961 atcgccgagc tccaggccca gatcgcggag ctcaagatgc agctggccaa gaaagaggag
     4021 gagctccagg ccgccctggc cagagtggaa gaggaagctg cccagaagaa catggccctc
     4081 aagaagatcc gggagctgga atctcagatc tctgaactcc aggaagacct ggagtctgag
     4141 cgtgcttcca ggaataaagc tgagaagcag aaacgggacc ttggggaaga gctagaggcg
     4201 ctgaaaacag agttggagga cacgctggat tccacagctg cccagcagga gctcaggtca
     4261 aaacgtgagc aggaggtgaa catcctgaag aagaccctgg aggaggaggc caagacccac
     4321 gaggcccaga tccaggagat gaggcagaag cactcacagg ccgtggagga gctggcggag
     4381 cagctggagc agacgaagcg ggtgaaagca aacctcgaga aggcaaagca gactctggag
     4441 aacgagcggg gggagctggc caacgaggtg aaggtgctgc tgcagggcaa aggggactcg
     4501 gagcacaagc gcaagaaagt ggaggcgcag ctgcaggagc tgcaggtcaa gttcaacgag
     4561 ggagagcgcg tgcgcacaga gctggccgac aaggtcacca agctgcaggt ggagctggac
     4621 aacgtgaccg ggcttctcag ccagtccgac agcaagtcca gcaagctcac caaggacttc
     4681 tccgcgctgg agtcccagct gcaggacact caggagctgc tgcaggagga gaaccggcag
     4741 aagctgagcc tgagcaccaa gctcaagcag gtggaggacg agaagaattc cttccgggag
     4801 cagctggagg aggaggagga ggccaagcac aacctggaga agcagatcgc caccctccat
     4861 gcccaggtgg ccgacatgaa aaagaagatg gaggacagtg tggggtgcct ggaaactgct
     4921 gaggaggtga agaggaagct ccagaaggac ctggagggcc tgagccagcg gcacgaggag
     4981 aaggtggccg cctacgacaa gctggagaag accaagacgc ggctgcagca ggagctggac
     5041 cacctgctgg tggacctgga ccaccagcgc cagagcgcgt gcaacctgga gaagaagcag
     5101 aagaagtttg accagctcct ggcggaggag aagaccatct ctgccaagta tgcagaggag
     5161 cgcgaccggg ctgaggcgga ggcccgagag aaggagacca aggctctgtc gctggcccgg
     5221 gccctggagg aagccatgga gcagaaggcg gagctggagc ggctcaacaa gcagttccgc
     5281 acggagatgg aggaccttat gagctccaag gatgatgtgg gcaagagtgt ccacgagctg
     5341 gagaagtcca agcgggccct agagcagcag gtggaggaga tgaagacgca gctggaagag
     5401 ctggaggacg agctgcaggc caccgaagat gccaagctgc ggttggaggt caacctgcag
     5461 gccatgaagg cccagttcga gcgggacctg cagggccggg acgagcagag cgaggagaag
     5521 aagaagcagc tggtcagaca ggtgcgggag atggaggcag agctggagga cgagaggaag
     5581 cagcgctcga tggcagtggc cgcccggaag aagctggaga tggacctgaa ggacctggag
     5641 gcgcacatcg actcggccaa caagaaccgg gacgaagcca tcaaacagct gcggaagctg
     5701 caggcccaga tgaaggactg catgcgcgag ctggatgaca cccgcgcctc tcgtgaggag
     5761 atcctggccc aggccaaaga gaacgagaag aagctgaaga gcatggaggc cgagatgatc
     5821 cagttgcagg aggaactggc agccgcggag cgtgccaagc gccaggccca gcaggagcgg
     5881 gatgagctgg ctgacgagat cgccaacagc agcggcaaag gagccctggc gttagaggag
     5941 aagcggcgtc tggaggcccg catcgcccag ctggaggagg agctggagga ggagcagggc
     6001 aacacggagc tgatcaacga ccggctgaag aaggccaacc tgcagatcga ccagatcaac
     6061 accgacctga acctggagcg cagccacgcc cagaagaacg agaatgctcg gcagcagctg
     6121 gaacgccaga acaaggagct taaggtcaag ctgcaggaga tggagggcac tgtcaagtcc
     6181 aagtacaagg cctccatcac cgccctcgag gccaagattg cacagctgga ggagcagctg
     6241 gacaacgaga ccaaggagcg ccaggcagcc tgcaaacagg tgcgtcggac cgagaagaag
     6301 ctgaaggatg tgctgctgca ggtggatgac gagcggagga acgccgagca gtacaaggac
     6361 caggccgaca aggcatctac ccgcctgaag cagctcaagc ggcagctgga ggaggccgaa
     6421 gaggaggccc agcgggccaa cgcctcccgc cggaaactgc agcgcgagct ggaggacgcc
     6481 actgagacgg ccgatgccat gaaccgcgaa gtcagctccc taaagaacaa gctcaggcgc
     6541 ggggacctgc cgtttgtcgt gccccgccga atggcccgga aaggcgccgg ggatggctcc
     6601 gacgaagagg tagatggcaa agcggatggg gctgaggcca aacctgccga ataagaccca
     6661 gctttcttgt acaaagtggt gatatcaagc ttatcgatac cgtcgacctc gagggggggc
     6721 ccggtacctt aattaattaa ggtaccaggt aagtgtaccc aattcgccct atagtgagtc
     6781 gtattacaat tcactcgatc gcccttccca acagttgcgc agcctgaatg gcgaatggag
     6841 atccaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     6901 attcacagtc ccaaggctca tttcaggccc ctcagtcctc acagtctgtt catgatcata
     6961 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     7021 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     7081 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7141 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
     7201 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     7261 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     7321 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     7381 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     7441 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     7501 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     7561 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     7621 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7681 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7741 ataaatgctt caataatatt gaaaaaggaa gaatcctgag gcggaaagaa ccagctgtgg
     7801 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7861 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     7921 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     7981 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8041 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8101 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     8161 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     8221 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     8281 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     8341 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     8401 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     8461 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     8521 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     8581 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     8641 ggacgaagaa catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     8701 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     8761 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     8821 tcaggacata gcgttggcta cccgtgatat tgctgaagaa cttggcggcg aatgggctga
     8881 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     8941 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     9001 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     9061 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     9121 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     9181 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     9241 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     9301 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     9361 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     9421 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     9481 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     9541 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     9601 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     9661 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     9721 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     9781 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     9841 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     9901 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     9961 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    10021 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    10081 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    10141 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    10201 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    10261 accgcc
//
