LOCUS       sequence-361459-       11370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway expression vector encoding wild type MYH9 cDNA with 
            N-terminal V5 tag. For mammalian expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Panza E, Marini M, Pecci A, Giacopelli F, Bozzi V, Seri M, Balduini 
            C, Ravazzolo R
  TITLE     Transfection of the mutant MYH9 cDNA reproduces the most typical 
            cellular phenotype of MYH9-related disease in different cell lines.
  JOURNAL   Pathogenetics. 2008 Dec 1;1(1):5. doi: 10.1186/1755-8417-1-5.
  PUBMED    19046415
REFERENCE   2  (bases 1 to 11370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Pathogenetics."; date: "2008-12-1"; volume: "1(1):5. doi"; pages: "
            10.1186/1755-8417-1-5"
COMMENT     Sequence Label: pMYH9-WT-V5
FEATURES             Location/Qualifiers
     source          1..11370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             914..955
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             956..976
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    1007..1031
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6918..6942)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6961..6978)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6967..7191
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7237..7665
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7324..7343)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7534..7555
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7674..7694)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7679..8008
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7859..7994
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7921..7940
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8075..8869
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8129..8148)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8739..8758
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9043..9164
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9080..9099)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9134..9153
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9213..9229)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9213..9229)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9226..9248)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9237..9253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9261..9267,9268..9285,9286..9291))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9306..9327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9444..9461)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9615..10203)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9695..9714)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10374..11165,11166..11234))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10997..11016
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11235..11339)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttacc atgggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacggaaaa
      961 cctgtatttt cagggcccgg atccgtcgac gaattctgca gatatcacaa gtttgtacaa
     1021 aaaagcaggc tccatggcac agcaagctgc cgataagtat ctctatgtgg ataaaaactt
     1081 catcaacaat ccgctggccc aggccgactg ggctgccaag aagctggtat gggtgccttc
     1141 cgacaagagt ggctttgagc cagccagcct caaggaggag gtgggcgaag aggccatcgt
     1201 ggagctggtg gagaatggga agaaggtgaa ggtgaacaag gatgacatcc agaagatgaa
     1261 cccgcccaag ttctccaagg tggaggacat ggcagagctc acgtgcctca acgaagcctc
     1321 ggtgctgcac aacctcaagg agcgttacta ctcagggctc atctacacct attcaggcct
     1381 gttctgtgtg gtcatcaatc cttacaagaa cctgcccatc tactctgaag agattgtgga
     1441 aatgtacaag ggcaagaaga ggcacgagat gccccctcac atctatgcca tcacagacac
     1501 cgcctacagg agtatgatgc aagaccgaga agatcaatcc atcttgtgca ctggtgaatc
     1561 tggagctggc aagacggaga acaccaagaa ggtcatccag tatctggcgt acgtggcgtc
     1621 ctcgcacaag agcaagaagg accagggcga gctggagcgg cagctgctgc aggccaaccc
     1681 catcctggag gccttcggga acgccaagac cgtgaagaat gacaactcct cccgcttcgg
     1741 caaattcatt cgcatcaact ttgatgtcaa tggctacatt gttggagcca acattgagac
     1801 ttatcttttg gagaaatctc gtgctatccg ccaagccaag gaagaacgga ccttccacat
     1861 cttctattat ctcctgtctg gggctggaga gcacctgaag accgatctcc tgttggagcc
     1921 gtacaacaaa taccgcttcc tgtccaatgg acacgtcacc atccccgggc agcaggacaa
     1981 ggacatgttc caggagacca tggaggccat gaggattatg ggcatcccag aagaggagca
     2041 aatgggcctg ctgcgggtca tctcaggggt tcttcagctc ggcaacatcg tcttcaagaa
     2101 ggagcggaac actgaccagg cgtccatgcc cgacaacaca gctgcccaaa aggtgtccca
     2161 tctcttgggt atcaatgtga ccgatttcac cagaggaatc ctcaccccgc gcatcaaggt
     2221 gggacgggat tacgtccaga aggcgcagac taaagagcag gctgactttg ccatcgaggc
     2281 cttggccaag gcgacctatg agcggatgtt ccgctggctg gtgctgcgca tcaacaaggc
     2341 tctggacaag accaagaggc agggcgcctc cttcatcggg atcctggaca ttgccggctt
     2401 cgagatcttt gatctgaact cgtttgagca gctgtgcatc aattacacca atgagaagct
     2461 gcagcagctc ttcaaccaca ccatgttcat cctggagcag gaggagtacc agcgcgaggg
     2521 catcgagtgg aacttcatcg actttggcct cgacctgcag ccctgcatcg acctcattga
     2581 gaagccagca ggccccccgg gcattctggc cctgctggac gaggagtgct ggttccccaa
     2641 agccaccgac aagagcttcg tggagaaggt gatgcaggag cagggcaccc accccaagtt
     2701 ccagaagccc aagcagctga aggacaaagc tgatttctgc attatccact atgccggcaa
     2761 ggtggattac aaagctgacg agtggctgat gaagaacatg gatcccctga atgacaacat
     2821 cgccacactg ctccaccagt cctctgacaa gtttgtctcg gagctgtgga aggatgtgga
     2881 ccgcatcatc ggcctggacc aggtggccgg catgtcggag accgcactgc ccggggcctt
     2941 caagacgcgg aagggcatgt tccgcactgt ggggcagctt tacaaggagc agctggccaa
     3001 gctgatggct acgctgagga acacgaaccc caactttgtc cgctgcatca tccccaacca
     3061 cgagaagaag gccggcaagc tggacccgca tctcgtgctg gaccagctgc gctgcaacgg
     3121 tgttctcgag ggcatccgta tctgccgcca gggcttcccc aacagggtgg tcttccagga
     3181 gtttcggcag agatatgaga tcctgactcc aaactccatt cccaagggtt tcatggacgg
     3241 gaagcaggcg tgcgtgctca tgataaaagc cctggagctc gacagcaatc tgtaccgcat
     3301 tggccagagc aaagtcttct tccgtgccgg tgtgctggcc cacctggagg aggagcgaga
     3361 cctgaagatc accgacgtca tcatagggtt ccaggcctgc tgcaggggct acctggccag
     3421 gaaagcattt gccaagcggc agcagcagct taccgccatg aaggtcctcc agcggaactg
     3481 cgctgcctac ctgaagctgc ggaactggca gtggtggcgg ctcttcacca aggtcaagcc
     3541 gctgctgcag gtgagccggc aggaggagga gatgatggcc aaggaggagg agctggtgaa
     3601 ggtcagagag aagcagctgg ctgcggagaa caggctcacg gagatggaga cgctgcagtc
     3661 tcagctcatg gcagagaaat tgcagctgca ggagcagctc caggcagaaa ccgagctgtg
     3721 tgccgaggct gaggagctcc gggcccgcct gaccgccaag aagcaggaat tagaagagat
     3781 ctgccatgac ctagaggcca gggtggagga ggaggaggag cgctgccagc acctgcaggc
     3841 ggagaagaag aagatgcagc agaacatcca ggagcttgag gagcagctgg aggaggagga
     3901 gagcgcccgg cagaagctgc agctggagaa ggtgaccacc gaggcgaagc tgaaaaagct
     3961 ggaggaggag cagatcatcc tggaggacca gaactgcaag ctggccaagg aaaagaaact
     4021 gctggaagac agaatagctg agttcaccac caacctcaca gaagaggagg agaaatctaa
     4081 gagcctcgcc aagctcaaga acaagcatga ggcaatgatc actgacttgg aagagcgcct
     4141 ccgcagggag gagaagcagc gacaggagct ggagaagacc cgccggaagc tggagggaga
     4201 ctccacagac ctcagcgacc agatcgccga gctccaggcc cagatcgcgg agctcaagat
     4261 gcagctggcc aagaaagagg aggagctcca ggccgccctg gccagagtgg aagaggaagc
     4321 tgcccagaag aacatggccc tcaagaagat ccgggagctg gaatctcaga tctctgaact
     4381 ccaggaagac ctggagtctg agcgtgcttc caggaataaa gctgagaagc agaaacggga
     4441 ccttggggaa gagctagagg cgctgaaaac agagttggag gacacgctgg attccacagc
     4501 tgcccagcag gagctcaggt caaaacgtga gcaggaggtg aacatcctga agaagaccct
     4561 ggaggaggag gccaagaccc acgaggccca gatccaggag atgaggcaga agcactcaca
     4621 ggccgtggag gagctggcgg agcagctgga gcagacgaag cgggtgaaag caaacctcga
     4681 gaaggcaaag cagactctgg agaacgagcg gggggagctg gccaacgagg tgaaggtgct
     4741 gctgcagggc aaaggggact cggagcacaa gcgcaagaaa gtggaggcgc agctgcagga
     4801 gctgcaggtc aagttcaacg agggagagcg cgtgcgcaca gagctggccg acaaggtcac
     4861 caagctgcag gtggagctgg acaacgtgac cgggcttctc agccagtccg acagcaagtc
     4921 cagcaagctc accaaggact tctccgcgct ggagtcccag ctgcaggaca ctcaggagct
     4981 gctgcaggag gagaaccggc agaagctgag cctgagcacc aagctcaagc aggtggagga
     5041 cgagaagaat tccttccggg agcagctgga ggaggaggag gaggccaagc acaacctgga
     5101 gaagcagatc gccaccctcc atgcccaggt ggccgacatg aaaaagaaga tggaggacag
     5161 tgtggggtgc ctggaaactg ctgaggaggt gaagaggaag ctccagaagg acctggaggg
     5221 cctgagccag cggcacgagg agaaggtggc cgcctacgac aagctggaga agaccaagac
     5281 gcggctgcag caggagctgg acgacctgct ggtggacctg gaccaccagc gccagagcgc
     5341 gtgcaacctg gagaagaagc agaagaagtt tgaccagctc ctggcggagg agaagaccat
     5401 ctctgccaag tatgcagagg agcgcgaccg ggctgaggcg gaggcccgag agaaggagac
     5461 caaggctctg tcgctggccc gggccctgga ggaagccatg gagcagaagg cggagctgga
     5521 gcggctcaac aagcagttcc gcacggagat ggaggacctt atgagctcca aggatgatgt
     5581 gggcaagagt gtccacgagc tggagaagtc caagcgggcc ctagagcagc aggtggagga
     5641 gatgaagacg cagctggaag agctggagga cgagctgcag gccaccgaag atgccaagct
     5701 gcggttggag gtcaacctgc aggccatgaa ggcccagttc gagcgggacc tgcagggccg
     5761 ggacgagcag agcgaggaga agaagaagca gctggtcaga caggtgcggg agatggaggc
     5821 agagctggag gacgagagga agcagcgctc gatggcagtg gccgcccgga agaagctgga
     5881 gatggacctg aaggacctgg aggcgcacat cgactcggcc aacaagaacc gggacgaagc
     5941 catcaaacag ctgcggaagc tgcaggccca gatgaaggac tgcatgcgcg agctggatga
     6001 cacccgcgcc tctcgtgagg agatcctggc ccaggccaaa gagaacgaga agaagctgaa
     6061 gagcatggag gccgagatga tccagttgca ggaggaactg gcagccgcgg agcgtgccaa
     6121 gcgccaggcc cagcaggagc gggatgagct ggctgacgag atcgccaaca gcagcggcaa
     6181 aggagccctg gcgttagagg agaagcggcg tctggaggcc cgcatcgccc agctggagga
     6241 ggagctggag gaggagcagg gcaacacgga gctgatcaac gaccggctga agaaggccaa
     6301 cctgcagatc gaccagatca acaccgacct gaacctggag cgcagccacg cccagaagaa
     6361 cgagaatgct cggcagcagc tggaacgcca gaacaaggag cttaaggtca agctgcagga
     6421 gatggagggc actgtcaagt ccaagtacaa ggcctccatc accgccctcg aggccaagat
     6481 tgcacagctg gaggagcagc tggacaacga gaccaaggag cgccaggcag cctgcaaaca
     6541 ggtgcgtcgg accgagaaga agctgaagga tgtgctgctg caggtggatg acgagcggag
     6601 gaacgccgag cagtacaagg accaggccga caaggcatct acccgcctga agcagctcaa
     6661 gcggcagctg gaggaggccg aagaggaggc ccagcgggcc aacgcctccc gccggaaact
     6721 gcagcgcgag ctggaggacg ccactgagac ggccgatgcc atgaaccgcg aagtcagctc
     6781 cctaaagaac aagctcaggc gcggggacct gccgtttgtc gtgccccgcc gaatggcccg
     6841 gaaaggcgcc ggggatggct ccgacgaaga ggtagatggc aaagcggatg gggctgaggc
     6901 caaacctgcc gaataagacc cagctttctt gtacaaagtg gtgataaacc cgctgatcag
     6961 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     7021 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     7081 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     7141 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     7201 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     7261 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     7321 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     7381 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7441 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7501 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7561 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7621 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     7681 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7741 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     7801 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7861 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7921 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     7981 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     8041 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     8101 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     8161 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     8221 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     8281 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     8341 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     8401 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     8461 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     8521 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     8581 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     8641 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     8701 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     8761 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     8821 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     8881 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     8941 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     9001 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     9061 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9121 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     9181 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     9241 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     9301 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9361 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9421 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9481 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9541 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9601 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9661 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9721 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9781 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9841 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9901 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9961 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    10021 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    10081 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10141 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10201 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10261 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    10321 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10381 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10441 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10501 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10561 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10621 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10681 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10741 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10801 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10861 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10921 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10981 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    11041 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    11101 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    11161 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    11221 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    11281 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    11341 acatttcccc gaaaagtgcc acctgacgtc
//
