LOCUS       sequence-36146-e         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  TITLE     Sekelsky lab Drosophila phiC31 cloning plasmids
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDEST-attB
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(109..233)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     256..272
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     257..273
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(310..326)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(310..326)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    544..613
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
       61 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
      121 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      181 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgatggg
      241 gatccgaatt cgagctcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc
      301 ccgggcaggg atccactagt tctagaattc gagctcccgg gtactcgagg tcgacatgcc
      361 cgccgtgacc gtcgagaacc cgctgacgct gccccgcgta tccgcacccg ccgacgccgt
      421 cgcacgtccc gtgctcaccg tgaccaccgc gcccagcggt ttcgagggcg agggcttccc
      481 ggtgcgccgc gcgttcgccg ggatcaacta ccgccacctc gacccgttca tcatgatgga
      541 ccagatgggt gaggtggagt acgcgcccgg ggagcccaag ggcacgccct ggcacccgca
      601 ccgcggcttc gagaccgtga cctacatcgt c
//
