LOCUS       sequence-361462-        1807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 2 promoter part for golden gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Leonard SP, Powell JE, Perutka J, Geng P, Heckmann LC, Horak RD, 
            Davies BW, Ellington AD, Barrick JE, Moran NA
  TITLE     Engineered symbionts activate honey bee immunity and limit 
            pathogens.
  JOURNAL   Science. 2020 Jan 31;367(6477):573-576. doi: 
            10.1126/science.aax9039. Epub 2020 Jan 30.
  PUBMED    32001655
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aax9039"; journalName: "Science"; date: 
            "2020-01-31- 31"; volume: "367"; issue: "6477"; pages: "573-576"
COMMENT     Sequence Label: pBTK150
FEATURES             Location/Qualifiers
     source          1..1807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(337..371)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(442..1101)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1016..1035
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1283..1807,1..64)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1772..1791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
       61 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      121 catgttcttt cctgcgttat cccctgattc tgtggataac cgtagtcggt ctcaaacgga
      181 cagtcattca tctttctgcc cctccaaaag caaaaacccg ccgaagcggg tttttacgta
      241 aatcaggtga aactgaccga ctttggcagt ttattcttga catgtagtga gggggctggt
      301 ataatcacat agtactgttt atgtgagacc agaccaataa aaaacgcccg gcggcaaccg
      361 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      421 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa
      481 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
      541 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
      601 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
      661 ggctgaaacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
      721 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
      781 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
      841 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
      901 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
      961 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     1021 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     1081 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     1141 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc
     1201 gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1261 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1321 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1381 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1441 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1501 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1561 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1621 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1681 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1741 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1801 tcgggtt
//
