LOCUS       sequence-361470-        8035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dsRNA targeting the InR1 gene from Apis mellifera.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Leonard SP, Powell JE, Perutka J, Geng P, Heckmann LC, Horak RD, 
            Davies BW, Ellington AD, Barrick JE, Moran NA
  TITLE     Engineered symbionts activate honey bee immunity and limit 
            pathogens.
  JOURNAL   Science. 2020 Jan 31;367(6477):573-576. doi: 
            10.1126/science.aax9039. Epub 2020 Jan 30.
  PUBMED    32001655
REFERENCE   2  (bases 1 to 8035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aax9039"; journalName: "Science"; date: 
            "2020-01-31- 31"; volume: "367"; issue: "6477"; pages: "573-576"
COMMENT     Sequence Label: pBTK1023 (pDS-InR1)
FEATURES             Location/Qualifiers
     source          1..8035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            2091..2178
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      2520..2914
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      4424..4495
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4511..4538
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(4557..5348)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(5728..6579)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(6566..7405)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(join(7916..8035,1..852))
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
ORIGIN
        1 ctcgatgtaa tccgcttcgt ggccgggctt gcgctctgcc agcgctgggc tggcctcggc
       61 catggccttg ccgatttcct cggcactgcg gccccggctg gccagcttct gcgcggcgat
      121 aaagtcgcac ttgctgaggt catcaccgaa gcgcttgacc agcccggcca tctcgctgcg
      181 gtactcgtcc agcgccgtgc gccggtggcg gctaagctgc cgctcgggca gttcgaggct
      241 ggccagcctg cgggccttct cctgctgccg ctgggcctgc tcgatctgct ggccagcctg
      301 ctgcaccagc gccgggccag cggtggcggt cttgcccttg gattcacgca gcagcaccca
      361 cggctgataa ccggcgcggg tggtgtgctt gtccttgcgg ttggtgaagc ccgccaagcg
      421 gccatagtgg cggctgtcgg cgctggccgg gtcggcgtcg tactcgctgg ccagcgtccg
      481 ggcaatctgc ccccgaagtt caccgcctgc ggcgtcggcc accttgaccc atgcctgata
      541 gttcttcggg ctggtttcca ctaccagggc aggctcccgg ccctcggctt tcatgtcatc
      601 caggtcaaac tcgctgaggt cgtccaccag caccagacca tgccgctcct gctcggcggg
      661 cctgatatac acgtcattgc cctgggcatt catccgcttg agccatggcg tgttctggag
      721 cacttcggcg gctgaccatt cccggttcat catctggccg gtggtggcgt ccctgacgcc
      781 gatatcgaag cgctcacagc ccatggcctt gagctgtcgg cctatggcct gcaaagtcct
      841 gtcgttcttc atcgggccac caagcgcagc cagatcgagc cgtcctcggt tgtcagtggc
      901 gtcaggtcga gcaagagcaa cgatgcgatc agcagcacca ccgtaggcat catggaagcc
      961 agcatcacgg ttagccatag cttccagtgc cacccccgcg acgcgctccg ggcgctctgc
     1021 gcggcgctgc tcacctcggc ggctacctcc cgcaactctt tggccagctc cacccatgcc
     1081 gcccctgtct ggcgctgggc tttcagccac tccgccgcct gcgcctcgct ggcctgctgg
     1141 gtctggctca tgacctgccg ggcttcgtcg gccagtgtcg ccatgctctg ggccagcggt
     1201 tcgatctgct ccgctaactc gttgatgcct ctggatttct tcactctgtc gattgcgttc
     1261 atggtctatt gcctcccggt attcctgtaa gtcgatgatc tgggcgttgg cggtgtcgat
     1321 gttcagggcc acgtctgccc ggtcggtgcg gatgccccgg ccttccatct ccaccacgtt
     1381 cggccccagg tgaacaccgg gcaggcgctc gatgccctgc gcctcaagtg ttctgtggtc
     1441 aatgcgggcg tcgtggccag cccgctctaa tgcccggttg gcatggtcgg cccatgcctc
     1501 gcgggtctgc tcaagccatg ccttgggctt gagcgcttcg gtcttctgtg ccccgccctt
     1561 ctccggggtc ttgccgttgt accgcttgaa ccactgagcg gcgggccgct cgatgccgtc
     1621 attgatccgc tcggagatca tcaggtggca gtgcgggttc tcgccgccac cggcatggat
     1681 ggccagcgta tacggcaggc gctcggcacc ggtcaggtgc tgggcgaact cggacgccag
     1741 cgccttctgc tggtcgaggg tcagctcgac cggcagggca aattcgacct ccttgaacag
     1801 ccgcccattg gcgcgttcat acaggtcggc agcatcccag tagtcggcgg gccgctcgac
     1861 gaactccggc atgtgcccgg attcggcgtg caagacttca tccatgtcgc gggcatactt
     1921 gccttcgcgc tggatgtagt cggccttggc cctggccgat tggccgcccg acctgctgcc
     1981 ggttttcgcc gtaaggtgat aaatcgccat gctgcctcgc tgttgctttt gcttttcggc
     2041 tccatgcaat ggccctcgga gagcgcaccg cccgaagggt ggccgttagg ccagtttctc
     2101 gaagagaaac cggtaagtgc gccctcccct acaaagtagg gtcgggattg ccgccgctgt
     2161 gcctccatga tagcctacga gacagcacat taacaatggg gtgtcaagat ggttaagggg
     2221 agcaacaagg cggcggatcg gctggccaag ctcgaagaac aacgagcgcg aatcaatgcc
     2281 gaaattcagc gggtgcgggc aagggaacag cagcaagagc gcaagaacga aacaaggcgc
     2341 aaggtgctgg tgggggccat gattttggcc aaggtgaaca gcagcgagtg gccggaggat
     2401 cggctcatgg cggcaatgga tgcgtacctt gaacgcgacc acgaccgcgc cttgttcggt
     2461 ctgccgccac gccagaagga tgagccgggc tgaatgatcg accgagacag gccctgcggg
     2521 gctgcacacg cgcccccacc cttcgggtag ggggaaaggc cgctaaagcg gctaaaagcg
     2581 ctccagcgta tttctgcggg gtttggtgtg gggtttagcg ggctttgccc gcctttcccc
     2641 ctgccgcgca gcggtggggc ggtgtgtagc ctagcgcagc gaatagacca gctatccggc
     2701 ctctggccgg gcatattggg caagggcagc agcgccccac aagggcgctg ataaccgcgc
     2761 ctagtggatt attcttagat aatcatggat ggatttttcc aacaccccgc cagcccccgc
     2821 ccctgctggg tttgcaggtt tgggggcgtg acagttattg caggggttcg tgacagttat
     2881 tgcagggggg cgtgacagtt attgcagggg ttcgtgacag ttagtacggg agtgacgggc
     2941 actggctggc aatgtctagc aacggcaggc atttcggctg agggtaaaag aactttccgc
     3001 taagcgatag actgtatgta aacacagtat tgcaaggacg cggaacatgc ctcatgtggc
     3061 ggccaggacg gccagccggg atcgggatac tggtcgttac cagagccacc gacccgagca
     3121 aacccttctc tatcagatcg ttgacgagta ttacccggca ttcgctgcgc ttatggcaga
     3181 gcagggaaag gaattgccgg gctatgtgca acgggaattt gaagaatttc tccaatgcgg
     3241 gcggctggag catggctttc tacgggttcg ctgcgagtct tgccacgccg agcacctggt
     3301 cgctttcaga aatcaatcta aagtatatat gagtaaactt ggtctgacag gcccctgaat
     3361 tcgcatctag atggtagagc cacaaacagc cggtacaagc aacgatctcc aggaccatct
     3421 gaatcatgcg cggatgacac gaactcacga cggcgatcac agacattaac ccacagtaca
     3481 gacactgcga caacgtggca attcgtcgca ataccgtctc actgaactgg ccgataattg
     3541 cagacgaacg gacagtcatt catctttctg cccctccaaa agcaaaaacc cgccgaagcg
     3601 ggtttttacg taaatcaggt gaaactgacc gactttggca gtttattctt gacatgtagt
     3661 gagggggctg gtataatcac atagtactgt ttatgccaga ttcctcaccg ttatgtttat
     3721 gttcactatg tcgcaggcca ccttctcccc gttcgactgg gattgcacct cgagctcggt
     3781 gaagttggat atgttcacca ttctgccgaa ttccacgatc ttggacaggc acagtttagg
     3841 gttgtaatgg aagaacagtc ggccgttctc gatcctgatc ctctgctcgg gcgggaacag
     3901 gctcgacaga ttcggattgt cgagcacgga caggctcgcg ttgtttatgt ccaactgctc
     3961 ccccttgatc accctcaacc tcttgaagaa gctcaacgag gtgatgggga acgagtgggt
     4021 gatcttcacg tactcggtta tctcctcgat ccggccgaac gcctcgctca actcgttcat
     4081 tatgttcgga ttatccaaca gtactatgtg attataccag ccccctcact acatgtcaag
     4141 aataaactgc caaaggtaat cgttaatccg caaataacgt aaaaacccgc ttcggcgggt
     4201 ttttttatgg ggggagttta gggaaagagc atttgtcagc tgagcatgag acggaaatct
     4261 gctcgtcagt ggtgctcaca ctgacgaatc atgtacagat cataccgatg actgcctggc
     4321 gactcacaac taagcaagac agccggaacc agcgccggcg aacaccactg catatatggc
     4381 atatcacaac agtccaacta gtgcactgca gtacaccagg catcaaataa aacgaaaggc
     4441 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     4501 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tataccgagc ggccgcttat
     4561 ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct
     4621 gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg
     4681 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
     4741 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     4801 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     4861 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     4921 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     4981 tcgaagatac ccgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     5041 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     5101 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     5161 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
     5221 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     5281 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     5341 tccctcataa tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     5401 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     5461 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     5521 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     5581 tacagcttac gaaccgaaca ggcttatgtc cactgggttc gtgccttcat ccgtttccac
     5641 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
     5701 gtcatgacca aaatccctta acgtgagtca gcctgccgcc ttgggccggg tgatgtcgta
     5761 cttgcccgcc gcgaactcgg ttaccgtcca gcccagcgcg accagctccg gcaacgcctc
     5821 gcgcacccgc tggcggcgct tgcgcatggt cgaaccactg gcctctgacg gccagacata
     5881 gccgcacaag gtatctatgg aagccttgcc ggttttgccg gggtcgatcc agccacacag
     5941 ccgctggtgc agcaggcggg cggtttcgct gtccagcgcc cgcacctcgt ccatgctgat
     6001 gcgcacatgc tggccgccac ccatgacggc ctgcgcgatc aaggggttca gggccacgta
     6061 caggcgcccg tccgcctcgt cgctggcgta ctccgacagc agccgaaacc cctgccgctt
     6121 gcggccattc tgggcgatga tggatacctt ccaaaggcgc tcgatgcagt cctgtatgtg
     6181 cttgagcgcc ccaccactat cgacctctgc cccgatttcc tttgccagcg cccgatagct
     6241 acctttgacc accatggcat cagcggtgac ggcctcccac ttgggttcca ggaacagccg
     6301 gagctgccgt ccgccttcgg tcttgggttc cgggccaagc actaggccat taggcccagc
     6361 catggccacc agcccttgca ggatgcgcag atcatcagcg cccagcggct ccgggccgct
     6421 gaactcgatc cgcttgccgt cgccgtagtc atacgtcacg tccagcttgc tgcgcttgcg
     6481 ctcgccccgc ttgagggcac ggaacaggcc gggggccaga cagtgcgccg ggtcgtgccg
     6541 gacgtggctg aggctgtgct tgttcttagg cttcaccacg gggcaccccc ttgctcttgc
     6601 gctgcctctc cagcacggcg ggcttgagca ccccgccgtc atgccgcctg aaccaccgat
     6661 cagcgaacgg tgcgccatag ttggccttgc tcacaccgaa gcggacgaag aaccggcgct
     6721 ggtcgtcgtc cacaccccat tcctcggcct cggcgctggt catgctcgac aggtaggact
     6781 gccagcggat gttatcgacc agtaccgagc tgccccggct ggcctgctgc tggtcgcctg
     6841 cgcccatcat ggccgcgccc ttgctggcat ggtgcaggaa cacgatagag cacccggtat
     6901 cggcggcgat ggcctccatg cgaccgatga cctgggccat ggggccgctg gcgttttctt
     6961 cctcgatgtg gaaccggcgc agcgtgtcca gcaccatcag gcggcggccc tcggcggcgc
     7021 gcttgaggcc gtcgaaccac tccggggcca tgatgttggg caggctgccg atcagcggct
     7081 ggatcagcag gccgtcagcc acggcttgcc gttcctcggc gctgaggtgc gccccaaggg
     7141 cgtgcaggcg gtgatgaatg gcggtgggcg ggtcttcggc gggcaggtag atcaccgggc
     7201 cggtgggcag ttcgcccacc tccagcagat ccggcccgcc tgcaatctgt gcggccagtt
     7261 gcagggccag catggattta ccggcaccac cgggcgacac cagcgccccg accgtaccgg
     7321 ccaccatgtt gggcaaaacg tagtccagcg gtggcggcgc tgctgcgaac gcctccagaa
     7381 tattgatagg cttatgggta gccattgatt gcctcctttg caggcagttg gtggttaggc
     7441 gctggcgggg tcactacccc cgccctgcgc cgctctgagt tcttccaggc actcgcgcag
     7501 cgcctcgtat tcgtcgtcgg tcagccagaa cttgcgctga cgcatccctt tggccttcat
     7561 gcgctcggca tatcgcgctt ggcgtacagc gtcagggctg gccagcaggt cgccggtctg
     7621 cttgtccttt tggtctttca tatcagtcac cgagaaactt gccggggccg aaaggcttgt
     7681 cttcgcggaa caaggacaag gtgcagccgt caaggttaag gctggccata tcagcgactg
     7741 aaaagcggcc agcctcggcc ttgtttgacg tataaccaaa gccaccgggc aaccaatagc
     7801 ccttgtcact tttgatcagg tagaccgacc ctgaagcgct tttttcgtat tccataaaac
     7861 ccccttctgt gcgtgagtac tcatagtata acaggcgtga gtaccaacgc aagcactaca
     7921 tgctgaaatc tggcccgccc ctgtccatgc ctcgctggcg gggtgccggt gcccgtgcca
     7981 gctcggcccg cgcaagctgg acgctgggca gacccatgac cttgctgacg gtgcg
//
