LOCUS       sequence-36148-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Burkholderia allelic exchange suicide vector pMo130, ref EU862243.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Hamad MA, Zajdowicz SL, Holmes RK, Voskuil MI
  TITLE     An allelic exchange system for compliant genetic manipulation of the
            select agents Burkholderia pseudomallei and Burkholderia mallei.
  JOURNAL   Gene. 2009 Feb 1. 430(1-2):123-31.
  PUBMED    19010402
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2009 
            Feb 1. 430(1-2):123-31."
COMMENT     Sequence Label: pMo130
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    148..164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
       61 tctatcgcct tcttgacgag ttcttctgat taattaaact agtgagctgt tgacaattaa
      121 tcatcgaatc tagtttaatg tgtggaattg tgagcggata acaattaagc gttaggagga
      181 aacgatgacg tactccaaac aagccgtttc ccgtagacac aaacgtttgg ccctgagcgc
      241 cgccgcgctc accgcagccg cttgcatgtc ggcgcacgcg cagagcgacg gcgcaactca
      301 agcgtttgcg gaagaaacga accaaaagcc atataaggaa acatacggca tttcccatat
      361 tacacgccat gatatgctgc aaatccctga acagcaaaaa aatgaaaaat atcaagtttc
      421 tgaatttgat tcgtccacaa ttaaaaatat ctcttctgca aaaggcctgg acgtttggga
      481 cagctggcca ttacaaaacg ctgacggcac tgtcgcaaac tatcacggct accacatcgt
      541 ctttgcatta gccggagatc ctaaaaatgc ggnngacaca tcgatttaca tgttctatca
      601 aaaagtcggc gaaacttcta ttgacagctg gaaaaacgct ggccgcgtct ttaaagacag
      661 cgacaaattc gatgcaaatg attctatcct aaaagaccaa acacaagaat ggtcaggttc
      721 agccacattt acatctgacg gaaaaatccg tttattctac actgatttct ccggtaaaca
      781 ttacggcaaa caaacactga caactgcaca agttaacgta tcagcatcag acagctcttt
      841 gaacatcaac ggtgtagagg attataaatc aatctttgac ggtgacggaa aaacgtatca
      901 aaatgtacag cagttcatcg atgaaggcaa ctacagctca
//
