LOCUS       sequence-361488-        2608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TC1
FEATURES             Location/Qualifiers
     source          1..2608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     677..693
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     677..693
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     698..717
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        698..716
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(891..2081)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1710..1729
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2129..2134,2135..2151,2152..2157))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2284..2608,1..264))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2364..2383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
       61 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      121 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      181 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      241 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      301 cagtggaacg acgcgtaact cacgttaagg gattttggtc atgggtggct cgaccagata
      361 tggtctcgcc ccggagcagg tggtggccat cgctagtaat gggggtggca aacaggctct
      421 tgagactgtt cagcgccttc taccagttct ctgtcaagcc cacggcctga cgcccgagca
      481 agttgtagcg attgctagtc atgacggtgg caaacaggct cttgaaaccg tccaacgcct
      541 tctaccagtt ctctgtcaag cccacggact aaccccagcg caggtgagac cagctttggt
      601 ctagaggagg catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
      661 cacgggccag agctgccagg aaacagctat gaccatgtaa tacgactcac tataggggat
      721 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
      781 gttcttcccc gatcaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc
      841 tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag
      901 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
      961 cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg
     1021 atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt
     1081 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     1141 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc
     1201 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
     1261 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
     1321 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
     1381 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
     1441 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     1501 aagggcatgg gtcggcgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
     1561 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgtaagga gatggcgccc
     1621 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
     1681 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     1741 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagagaat ccacaggacg
     1801 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
     1861 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc
     1921 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
     1981 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
     2041 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
     2101 tagcaattta actgtgataa actaccgcat taaagctaat cgatgataag ctgtcaaaca
     2161 tgagaattaa ttcatgttct ttcctgcgtt atcccctgat tctgtggata acgcaggaaa
     2221 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2281 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2341 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2401 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2461 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2521 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2581 taactatcgt cttgagtcca acccggta
//
