LOCUS       sequence-361491-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GG9
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     313..329
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     313..329
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     334..353
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        334..352
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(527..1717)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1346..1365
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1765..1770,1771..1787,1788..1793))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(1920..2508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2000..2019)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatatggtct caagaggcaa tcgctacaac ttgctccgga gtcagaccgt gggcttgaca
       61 gagaactggc agtaggcgct gtacggtttc aagagcctgt ttgccaccat tgttactagc
      121 aatcgctaca acttgctccg gagtcagacc gtgggcttga cagagaactg gcagtaggcg
      181 ctgtacggtt tcaagagcct gtttgccacc attgttggaa cgagaccagc tttggtctag
      241 aggaggcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg
      301 ggccagagct gccaggaaac agctatgacc atgtaatacg actcactata ggggatattt
      361 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
      421 ttccccgatc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca
      481 attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg
      541 cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta
      601 caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca
      661 gcggtccaat gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct
      721 gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc
      781 cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca
      841 agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac
      901 gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg
      961 caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc
     1021 agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg
     1081 cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg
     1141 gcatgggtcg gcgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     1201 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg taaggagatg gcgcccaaca
     1261 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     1321 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     1381 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gagaatccac aggacgggtg
     1441 tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc
     1501 ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg
     1561 catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc
     1621 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
     1681 tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
     1741 aatttaactg tgataaacta ccgcattaaa gctaatcgat gataagctgt caaacatgag
     1801 aattaattca tgttctttcc tgcgttatcc cctgattctg tggataacgc aggaaagaac
     1861 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1921 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1981 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2041 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2101 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2161 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2221 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2281 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2341 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2401 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2461 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2521 atcttttcta cggggtctga cgctcagtgg aacgacgcgt aactcacgtt aagggatttt
     2581 ggtcatgggt ggctcgacca
//
