LOCUS       sequence-36150-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Gallagher JE, Dunbar DA, Granneman S, Mitchell BM, Osheim Y, Beyer 
            AL, Baserga SJ
  TITLE     RNA polymerase I transcription and pre-rRNA processing are linked by
            specific SSU processome components.
  JOURNAL   Genes Dev. 2004 Oct 15. 18(20):2506-17.
  PUBMED    15489292
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2004 Oct 15. 18(20):2506-17."
COMMENT     Sequence Label: pTOPO-rDNA-NTS
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(442..461)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(443..461)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(468..485)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(468..484)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(477..499)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(712..731)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctcggatcc actagtaacg gccgccagtg tgctggaatt cgcccttctt cataacctgt
       61 caccttgaaa ctacctctgc atgccaccta ccgaccaacc ttcatgttct gtttcgacct
      121 acctcttgta aatgacaaat cacctttttc atcgtatgca ccttattctc cacatcacaa
      181 tgcactattg cttttgcttt tccacctgtc atatcctatt gctattagat gaaatataat
      241 aaaaattgtc ctccacccat aacacctctc actcccacct actgaacatg cctggaccct
      301 gccctcatat cacctgcgtt tccgttaaac tatcggttgc ggccatatct accaaggtga
      361 caggttatga agaagggcga attctgcaga tatccatcac actggcggcc gctcgagcat
      421 gcatctagag ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
      481 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      541 ccccctttcg ccagctggcg taatancgaa gaggcccgca ccgatcgccc ttcccaacag
      601 ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      661 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      721 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      781 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
      841 agggtgatgg ttcacgtagt
//
