LOCUS       sequence-36151-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Gallagher JE, Dunbar DA, Granneman S, Mitchell BM, Osheim Y, Beyer 
            AL, Baserga SJ
  TITLE     RNA polymerase I transcription and pre-rRNA processing are linked by
            specific SSU processome components.
  JOURNAL   Genes Dev. 2004 Oct 15. 18(20):2506-17.
  PUBMED    15489292
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2004 Oct 15. 18(20):2506-17."
COMMENT     Sequence Label: pTOPO-rRNA-5'ETS 1 to 177
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(355..374)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(356..374)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(381..398)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(381..397)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(390..412)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(625..644)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctcggatcc actagtaacg gccgccagtg tgctggaatt cgcccttaat acgcatcaac
       61 ccatgtcaat taaacacgct gtatagagac taggcagatc tgacgatcac ctagcgactc
      121 tctccaccgt ttgacgaggc catttacaaa aacataacga acgacaagcc tactcgaatt
      181 cgtttccaaa ctcttttcga acttgtcttc aactgctttc gcatgaagta cctcccaact
      241 acttttcctc acacttgtac tccatgacta aacccccccc tccataaggg cgaattctgc
      301 agatatccat cacactggcg gccgctcgag catgcatcta gagggcccaa ttcgccctat
      361 agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      421 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      481 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
      541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      661 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
      721 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
      781 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aata
//
