LOCUS       sequence-361538-        2468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-hG8
FEATURES             Location/Qualifiers
     source          1..2468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     381..400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(607..612,613..629,630..635)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             683..1873
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1035..1054)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2047..2066)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2048..2066)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2071..2087)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2071..2087)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2360..2468,1..480)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
       61 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      121 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      181 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      241 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      301 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      361 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      421 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      481 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      541 ttctttcctg cgttatccac agaatcaggg gataacgcag gaaagaacat gaattaattc
      601 tcatgtttga cagcttatca tcgattagct ttaatgcggt agtttatcac agttaaattg
      661 ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac
      721 cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg
      781 ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc
      841 gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg
      901 cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac
      961 acccgtcctg tggattctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
     1021 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
     1081 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
     1141 gttgggcgcc atctccttac atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     1201 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgcc gacccatgcc
     1261 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     1321 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg
     1381 ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc
     1441 ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg
     1501 tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt
     1561 gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg
     1621 cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca
     1681 gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca ttggaccgct
     1741 gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt
     1801 aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc
     1861 cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt
     1921 ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg atcggggaag
     1981 aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa
     2041 aatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     2101 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc
     2161 tagaccaaag ctggtctcac gcggccaacg ccggatcagg gcgagataac tgggcaacaa
     2221 tgctctccag cgccggcctg ccgccattgt tacttcgaga ccatatctgg tcgagccacc
     2281 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     2341 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2401 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2461 ttccgaag
//
