LOCUS       sequence-361539-        2468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-hG9
FEATURES             Location/Qualifiers
     source          1..2468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     162..181
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(388..393,394..410,411..416)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             464..1654
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(816..835)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1828..1847)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1829..1847)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1852..1868)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1852..1868)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2141..2468,1..261)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
       61 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      121 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      181 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      241 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
      301 tgctggcctt ttgctcacat gttctttcct gcgttatcca cagaatcagg ggataacgca
      361 ggaaagaaca tgaattaatt ctcatgtttg acagcttatc atcgattagc tttaatgcgg
      421 tagtttatca cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc
      481 gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc
      541 ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca gtcactatgg
      601 cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc tcggagcact
      661 gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag ccactatcga
      721 ctacgcgatc atggcgacca cacccgtcct gtggattctc tacgccggac gcatcgtggc
      781 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
      841 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
      901 aggccccgtg gccgggggac tgttgggcgc catctcctta catgcaccat tccttgcggc
      961 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     1021 gggagagcgc cgacccatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     1081 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     1141 acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac
     1201 gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt
     1261 cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc
     1321 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat
     1381 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag
     1441 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct
     1501 aacttcgatc attggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg
     1561 gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg
     1621 tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac
     1681 ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa
     1741 accaaccctt gatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
     1801 agcctgattg ggagaaaata aaatatcccc tatagtgagt cgtattacat ggtcatagct
     1861 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     1921 aatatatcat catgcctcct ctagaccaaa gctggtctcg ttccaacaat ggcggcaggc
     1981 cggcgctgga gagcattgtt gcccagttat ctcgccctga tccggcgttg gccgcgtgag
     2041 accatatctg gtcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     2101 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2161 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2221 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2281 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2341 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2401 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2461 cggggggt
//
