LOCUS       sequence-361541-        2608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GT1
FEATURES             Location/Qualifiers
     source          1..2608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     347..363
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     347..363
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     368..387
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        368..386
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(561..1751)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1380..1399
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1799..1804,1805..1821,1822..1827))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(1954..2542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2034..2053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gattttggtc atgggtggct cgaccagata tggtctcacc tgctcgggcg tcaggccgtg
       61 ggcttgacag agaactggta gaaggcgctg aacagtctca agagcctgtt tgccaccccc
      121 attactagca atcgctacaa cttgctccgg agtcagaccg tgggcttgac agagaactgg
      181 cagtaggcgc tgtacggttt caagagcctg tttgccacca ttgttactag cgatggccac
      241 cacctgctcc ggggcgagac cagctttggt ctagaggagg catgatgata tatttttatc
      301 ttgtgcaatg taacatcaga gattttgaga cacgggccag agctgccagg aaacagctat
      361 gaccatgtaa tacgactcac tataggggat attttatttt ctcccaatca ggcttgatcc
      421 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatcaagggt tggtttgcgc
      481 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
      541 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
      601 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg
      661 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg
      721 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
      781 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
      841 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc
      901 atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag
      961 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
     1021 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
     1081 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
     1141 cggaaggagc tgactgggtt gaaggctctc aagggcatgg gtcggcgctc tcccttatgc
     1201 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
     1261 aggaatggtg catgtaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     1321 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     1381 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     1441 atgcgtccgg cgtagagaat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata
     1501 gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct
     1561 ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag
     1621 tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata
     1681 accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg
     1741 ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat
     1801 taaagctaat cgatgataag ctgtcaaaca tgagaattaa ttcatgttct ttcctgcgtt
     1861 atcccctgat tctgtggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1921 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1981 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2041 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2101 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2161 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2221 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2281 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2341 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2401 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2461 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2521 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2581 gtggaacgac gcgtaactca cgttaagg
//
