LOCUS       sequence-361542-        2604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GT2
FEATURES             Location/Qualifiers
     source          1..2604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(531..536,537..553,554..559)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             607..1797
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(959..978)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1971..1990)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1972..1990)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1995..2011)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1995..2011)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2420..2604,1..404)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
       61 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      121 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      181 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      241 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      301 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      361 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      421 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      481 ccacagaatc aggggataac gcaggaaaga acatgaatta attctcatgt ttgacagctt
      541 atcatcgatt agctttaatg cggtagttta tcacagttaa attgctaacg cagtcaggca
      601 ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct
      661 gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat cgtccattcc
      721 gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta
      781 tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct
      841 tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatt
      901 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
      961 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     1021 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     1081 ttacatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     1141 ttcctaatgc aggagtcgca taagggagag cgccgaccca tgcccttgag agccttcaac
     1201 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     1261 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag
     1321 gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg
     1381 cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag
     1441 gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg
     1501 cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc
     1561 gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga
     1621 tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt cacggcgatt
     1681 tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac
     1741 cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg
     1801 gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct
     1861 tgcggagaac tgtgaatgcg caaaccaacc cttgatcggg gaagaacagt atgtcgagct
     1921 attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatc ccctatagtg
     1981 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     2041 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc aaagctggtc
     2101 tcgcaggttg tagcgattgc tagtaacaat ggtggcaaac aggctcttga aaccgtacag
     2161 cgcctactgc cagttctctg tcaagcccac ggtctgactc cggagcaagt tgtagcgatt
     2221 gctagtaatg ggggtggcaa acaggctctt gagactgttc agcgccttct accagttctc
     2281 tgtcaagccc acggcctgac gcccgagcaa gtggttgaga ccatatctgg tcgagccacc
     2341 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     2401 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2461 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2521 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2581 cgtagttagg ccaccacttc aaga
//
