LOCUS       sequence-361544-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GT3
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(47..635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(127..146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1042..1058
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1042..1058
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1063..1082
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1063..1081
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1256..2446)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2075..2094
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2494..2499,2500..2516,2517..2522))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
       61 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      121 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      181 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      241 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      301 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      361 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      421 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      481 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      541 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      601 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
      661 ggtctgacgc tcagtggaac gacgcgtaac tcacgttaag ggattttggt catgggtggc
      721 tcgaccagat atggtctcgt ggtagcgatt gctagtaaca atggtggcaa acaggctctt
      781 gaaaccgtac agcgcctact gccagttctc tgtcaagccc acggtctgac tccggagcaa
      841 gttgtagcga ttgctagtaa tgggggtggc aaacaggctc ttgagactgt tcagcgcctt
      901 ctaccagttc tctgtcaagc ccacggcctg acgcccgagc aagttgtagt gagaccagct
      961 ttggtctaga ggaggcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
     1021 tgagacacgg gccagagctg ccaggaaaca gctatgacca tgtaatacga ctcactatag
     1081 gggatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac
     1141 atactgttct tccccgatca agggttggtt tgcgcattca cagttctccg caagaattga
     1201 ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg
     1261 tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg
     1321 cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg
     1381 tgacgatcag cggtccaatg atcgaagtta ggctggtaag agccgcgagc gatccttgaa
     1441 gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc
     1501 cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga
     1561 acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct
     1621 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc
     1681 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga
     1741 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca
     1801 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
     1861 ctctcaaggg catgggtcgg cgctctccct tatgcgactc ctgcattagg aagcagccca
     1921 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgt aaggagatgg
     1981 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     2041 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     2101 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag agaatccaca
     2161 ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca
     2221 ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt
     2281 gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga
     2341 cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
     2401 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
     2461 tgcgttagca atttaactgt gataaactac cgcattaaag ctaatcgatg ataagctgtc
     2521 aaacatgaga attaattcat gttctttcct gcgttatccc ctgattctgt ggataacgca
     2581 ggaaagaaca tgtgagcaaa ag
//
