LOCUS       sequence-361547-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GT4
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(48..636)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(128..147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1044..1060
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1044..1060
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1065..1084
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1065..1083
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1258..2448)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2077..2096
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2496..2501,2502..2518,2519..2524))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
       61 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      121 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      181 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      241 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      301 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      361 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      421 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      481 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      541 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      601 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      661 gggtctgacg ctcagtggaa cgacgcgtaa ctcacgttaa gggattttgg tcatgggtgg
      721 ctcgaccaga tatggtctca ttgctacaac ttgctcgggc gtcaggccgt gggcttgaca
      781 gagaactggt agaaggcgct gaacagtctc aagagcctgt ttgccacccc cattactagc
      841 aatcgctaca acttgctccg gagtcagacc gtgggcttga cagagaactg gcagtaggcg
      901 ctgtacggtt tcaagagcct gtttgccacc attgttacta gcaatcgcta ccgagaccag
      961 ctttggtcta gaggaggcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     1021 tttgagacac gggccagagc tgccaggaaa cagctatgac catgtaatac gactcactat
     1081 aggggatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
     1141 acatactgtt cttccccgat caagggttgg tttgcgcatt cacagttctc cgcaagaatt
     1201 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca
     1261 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag
     1321 ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc
     1381 cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg
     1441 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
     1501 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
     1561 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt
     1621 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
     1681 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
     1741 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
     1801 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
     1861 ggctctcaag ggcatgggtc ggcgctctcc cttatgcgac tcctgcatta ggaagcagcc
     1921 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gtaaggagat
     1981 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     2041 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     2101 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agagaatcca
     2161 caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag
     2221 caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa
     2281 ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg
     2341 gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
     2401 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
     2461 actgcgttag caatttaact gtgataaact accgcattaa agctaatcga tgataagctg
     2521 tcaaacatga gaattaattc atgttctttc ctgcgttatc ccctgattct gtggataacg
     2581 caggaaagaa catgtgagca aaa
//
