LOCUS       sequence-361549-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GT6
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     441..457
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     441..457
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     462..481
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        462..480
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(655..1845)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1474..1493
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1893..1898,1899..1915,1916..1921))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2048..2603,1..33))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2128..2147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
       61 tctgacgctc agtggaacga cgcgtaactc acgttaaggg attttggtca tgggtggctc
      121 gaccagatat ggtctcaggc tatcgctaca acttgctcgg gcgtcaggcc gtgggcttga
      181 cagagaactg gtagaaggcg ctgaacagtc tcaagagcct gtttgccacc cccattacta
      241 gcaatcgcta caacttgctc cggagtcaga ccgtgggctt gacagagaac tggcagtagg
      301 cgctgtacgg tttcaagagc ctgtttgcca ccattgttac tagcaatccg agaccagctt
      361 tggtctagag gaggcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt
      421 gagacacggg ccagagctgc caggaaacag ctatgaccat gtaatacgac tcactatagg
      481 ggatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
      541 tactgttctt ccccgatcaa gggttggttt gcgcattcac agttctccgc aagaattgat
      601 tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt
      661 cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc
      721 ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt
      781 gacgatcagc ggtccaatga tcgaagttag gctggtaaga gccgcgagcg atccttgaag
      841 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
      901 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa
      961 cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc
     1021 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
     1081 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
     1141 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
     1201 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     1261 tctcaagggc atgggtcggc gctctccctt atgcgactcc tgcattagga agcagcccag
     1321 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgta aggagatggc
     1381 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
     1441 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
     1501 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga gaatccacag
     1561 gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag
     1621 gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg
     1681 catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac
     1741 gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag
     1801 ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact
     1861 gcgttagcaa tttaactgtg ataaactacc gcattaaagc taatcgatga taagctgtca
     1921 aacatgagaa ttaattcatg ttctttcctg cgttatcccc tgattctgtg gataacgcag
     1981 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2041 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2101 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2161 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2221 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2281 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2341 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2401 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2461 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2521 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2581 gcggtggttt ttttgtttgc aag
//
