LOCUS       sequence-361555-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GT10
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(361..380)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(362..380)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(385..401)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(385..401)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      806..1394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1295..1314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1521..1526,1527..1543,1544..1549)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             join(1597..2600,1..187)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1949..1968)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt
       61 cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc
      121 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc
      181 gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc
      241 aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttgatcggg gaagaacagt
      301 atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatc
      361 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
      421 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc
      481 aaagctggtc tcatgacgca atcgctacaa cttgctcggg cgtcaggccg tgggcttgac
      541 agagaactgg tagaaggcgc tgaacagtct caagagcctg tttgccaccc ccattactag
      601 caatcgctac aacttgctcc ggagtcagac cgtgggcttg acagagaact ggcagtaggc
      661 gctgtacggt ttcaagagcc tgtttgccac cattgttaga gcgagaccat atctggtcga
      721 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
      781 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
      841 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
      901 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
      961 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1021 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1081 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1141 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     1201 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     1261 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1321 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1381 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     1441 cttttgctca catgttcttt cctgcgttat ccacagaatc aggggataac gcaggaaaga
     1501 acatgaatta attctcatgt ttgacagctt atcatcgatt agctttaatg cggtagttta
     1561 tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc
     1621 gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg
     1681 ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg
     1741 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac
     1801 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg
     1861 atcatggcga ccacacccgt cctgtggatt ctctacgccg gacgcatcgt ggccggcatc
     1921 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     1981 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     2041 gtggccgggg gactgttggg cgccatctcc ttacatgcac cattccttgc ggcggcggtg
     2101 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     2161 cgccgaccca tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     2221 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     2281 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     2341 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     2401 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     2461 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     2521 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     2581 gatgacgacc atcagggaca
//
