LOCUS       sequence-361560-        2608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TA1
FEATURES             Location/Qualifiers
     source          1..2608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(240..259)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(241..259)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(264..280)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(264..280)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      693..1281
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1182..1201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1408..1413,1414..1430,1431..1436)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             join(1484..2608,1..66)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1836..1855)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg
       61 acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca
      121 atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttgatcgggg aagaacagta
      181 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatcc
      241 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
      301 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
      361 aagctggtct cacctgatct ggggtgagtc cgtgggcttg acagagaact ggaaggaggc
      421 gctgaaccgt ctcaagagcc tgtttgccac caatattact agcaatcgct acaacttgct
      481 cgggcgtcag gccgtgggct tgacagagaa ctggtagaag gcgctgaaca gtctcaagag
      541 cctgtttgcc acccccatta ctagcgatgg ccaccacctg ctccggggcg agaccatatc
      601 tggtcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
      661 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      721 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      781 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      841 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      901 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      961 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1021 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     1081 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1141 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1201 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1261 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     1321 tgctggcctt ttgctcacat gttctttcct gcgttatcca cagaatcagg ggataacgca
     1381 ggaaagaaca tgaattaatt ctcatgtttg acagcttatc atcgattagc tttaatgcgg
     1441 tagtttatca cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc
     1501 gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc
     1561 ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca gtcactatgg
     1621 cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc tcggagcact
     1681 gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag ccactatcga
     1741 ctacgcgatc atggcgacca cacccgtcct gtggattctc tacgccggac gcatcgtggc
     1801 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     1861 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     1921 aggccccgtg gccgggggac tgttgggcgc catctcctta catgcaccat tccttgcggc
     1981 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     2041 gggagagcgc cgacccatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     2101 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     2161 acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac
     2221 gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt
     2281 cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc
     2341 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat
     2401 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag
     2461 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct
     2521 aacttcgatc attggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg
     2581 gaacgggttg gcatggattg taggcgcc
//
