LOCUS       sequence-361564-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TA4
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      410..998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     899..918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1125..1130,1131..1147,1148..1153)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1201..2391
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1553..1572)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2565..2584)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2566..2584)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(join(2589..2603,1..2))
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(2589..2603,1..2))
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
       61 tcatcatgcc tcctctagac caaagctggt ctcggtagcg attgctagta atgggggtgg
      121 caaacaggct cttgagactg ttcagcgcct tctaccagtt ctctgtcaag cccacggcct
      181 gacgcccgag caagttgtag cgattgctag taatattggt ggcaaacagg ctcttgagac
      241 ggttcagcgc ctccttccag ttctctgtca agcccacgga ctcaccccag atcaagttgt
      301 agcaatgaga ccatatctgg tcgagccacc catgaccaaa atcccttaac gtgagttacg
      361 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
      421 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      481 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
      541 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
      601 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
      661 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      721 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
      781 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
      841 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
      901 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
      961 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     1021 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatccacag
     1081 aatcagggga taacgcagga aagaacatga attaattctc atgtttgaca gcttatcatc
     1141 gattagcttt aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt
     1201 atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc
     1261 ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc
     1321 atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca
     1381 cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta
     1441 cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gattctctac
     1501 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     1561 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     1621 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttacat
     1681 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     1741 atgcaggagt cgcataaggg agagcgccga cccatgccct tgagagcctt caacccagtc
     1801 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     1861 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
     1921 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
     1981 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
     2041 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
     2101 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
     2161 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
     2221 gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc
     2281 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
     2341 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc
     2401 ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga
     2461 gaactgtgaa tgcgcaaacc aacccttgat cggggaagaa cagtatgtcg agctattttt
     2521 tgacttactg gggatcaagc ctgattggga gaaaataaaa tatcccctat agtgagtcgt
     2581 attacatggt catagctgtt tcc
//
