LOCUS       sequence-361566-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TA5
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     329..345
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     329..345
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     350..369
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        350..368
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(543..1733)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1362..1381
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1781..1786,1787..1803,1804..1809))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(1936..2524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2016..2035)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggtggctcg accagatatg gtctcaaatc gctacaactt gatctggggt gagtccgtgg
       61 gcttgacaga gaactggaag gaggcgctga accgtctcaa gagcctgttt gccaccaata
      121 ttactagcaa tcgctacaac ttgctcgggc gtcaggccgt gggcttgaca gagaactggt
      181 agaaggcgct gaacagtctc aagagcctgt ttgccacccc cattactagc aattgccgag
      241 accagctttg gtctagagga ggcatgatga tatattttta tcttgtgcaa tgtaacatca
      301 gagattttga gacacgggcc agagctgcca ggaaacagct atgaccatgt aatacgactc
      361 actatagggg atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
      421 gctcgacata ctgttcttcc ccgatcaagg gttggtttgc gcattcacag ttctccgcaa
      481 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc
      541 attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg
      601 tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa
      661 atcgccgtga cgatcagcgg tccaatgatc gaagttaggc tggtaagagc cgcgagcgat
      721 ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg
      781 ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc
      841 gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc
      901 tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc
      961 aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc
     1021 tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag
     1081 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg
     1141 ttgaaggctc tcaagggcat gggtcggcgc tctcccttat gcgactcctg cattaggaag
     1201 cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgtaag
     1261 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
     1321 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
     1381 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagaga
     1441 atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa
     1501 gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat
     1561 agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg
     1621 gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca
     1681 gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt
     1741 gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagcta atcgatgata
     1801 agctgtcaaa catgagaatt aattcatgtt ctttcctgcg ttatcccctg attctgtgga
     1861 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1921 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     1981 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2041 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2101 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2161 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2221 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2281 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2341 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2401 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2461 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2521 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     2581 cacgttaagg gattttggtc at
//
