LOCUS       sequence-361568-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TA7
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     449..468
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(675..680,681..697,698..703)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             751..1941
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1103..1122)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2115..2134)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2116..2134)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2139..2155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2139..2155)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2563..2603,1..548)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
       61 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      121 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      181 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      241 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      301 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      361 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      421 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      481 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      541 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      601 ctcacatgtt ctttcctgcg ttatccacag aatcagggga taacgcagga aagaacatga
      661 attaattctc atgtttgaca gcttatcatc gattagcttt aatgcggtag tttatcacag
      721 ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc
      781 ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc
      841 ctcttgcggg atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg
      901 ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt
      961 ggccgccgcc cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg
     1021 gcgaccacac ccgtcctgtg gattctctac gccggacgca tcgtggccgg catcaccggc
     1081 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     1141 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     1201 gggggactgt tgggcgccat ctccttacat gcaccattcc ttgcggcggc ggtgctcaac
     1261 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgccga
     1321 cccatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     1381 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     1441 gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg
     1501 tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc
     1561 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
     1621 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
     1681 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
     1741 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcatt
     1801 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca
     1861 tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
     1921 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc
     1981 caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttgat
     2041 cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga
     2101 gaaaataaaa tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     2161 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2221 gcctcctcta gaccaaagct ggtctcaact tgcaatcgct acaacttgat ctggggtgag
     2281 tccgtgggct tgacagagaa ctggaaggag gcgctgaacc gtctcaagag cctgtttgcc
     2341 accaatatta ctagcaatcg ctacaacttg ctcgggcgtc aggccgtggg cttgacagag
     2401 aactggtaga aggcgctgaa cagtctcaag agcctgtttg ccacccccat tactggctcg
     2461 agaccatatc tggtcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     2521 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2581 gcgcgtaatc tgctgcttgc aaa
//
