LOCUS       sequence-361597-       15027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector for mouse ROSA26 locus to create landing pad cell 
            line. Introduces attP50 sites flanking a Neo gene driven by 
            endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15027)
  AUTHORS   Malaguti M, Migueles RP, Annoh J, Sadurska D, Blin G, Lowell S
  TITLE     SyNPL: Synthetic notch pluripotent cell lines to monitor and 
            manipulate cell interactions in vitro and in vivo.
  JOURNAL   Development. 2022 May 26. pii: 275525. doi: 10.1242/dev.200226.
  PUBMED    35616331
REFERENCE   2  (bases 1 to 15027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2022 May 26. pii: 275525. doi: 10.1242/dev.200226."
COMMENT     Sequence Label: pmROSA26-attP50-Neo-mKate2-3xNLS-attP50
FEATURES             Location/Qualifiers
     source          1..15027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(14..33)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(15..33)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(43..60)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(43..59)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(52..74)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      201..629
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(288..307)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     498..519
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        682..786
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(787..855,856..1647)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1005..1024)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1818..2406
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2307..2326
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2560..2577
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2694..2715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2730..2735,2736..2753,2754..2760)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2768..2784
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2773..2795
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2792..2808
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2792..2808
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2827..2847
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2829..2847
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    2909..3133
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     intron          3207..3272
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(3349..3921)
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     promoter        complement(3948..4447)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3996..4015)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     4389..4412
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    4497..8837
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     polyA_signal    8891..9115
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    9162..9186
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        9266..9645
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          9923..10938
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10862..10884
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10946..10965
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      11002..11011
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11008..11703
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             11719..11739
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11743..11763
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11767..11787
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11844..12068
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    12117..12251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    12371..12505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(12757..13560)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12868..12887)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     13478..13497
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     regulatory      13557..13566
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(13643..13667)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(13723..13756)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(13770..13786)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13770..13786)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(13819..13856,13857..13922)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     misc_feature    13941..15023
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
ORIGIN
        1 gagctccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
       61 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      121 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      181 cascckraaw gkssmacstg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      241 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
      301 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
      361 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
      421 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
      481 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
      541 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
      601 gctgatttaa caaaaattta acgcgaattt taacaaaata ttmwmsmkkr crmktymsrt
      661 kssmcwttym ggskrmryrw scgcggaacc cctatttgtt tatttttcta aatacattca
      721 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      781 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      841 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      901 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      961 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     1021 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1081 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     1141 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1201 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     1261 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     1321 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     1381 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     1441 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     1501 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1561 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1621 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1681 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1741 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1801 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1861 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1921 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1981 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2041 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2101 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2161 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2221 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2281 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2341 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2401 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2461 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2521 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2581 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2641 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     2701 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     2761 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     2821 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg gtaccgggcc ccccctcgag
     2881 ccccagctgg ttctttccgc ctcagaagcc atagagccca ccgcatcccc agcatgcctg
     2941 ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa
     3001 tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga
     3061 gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa
     3121 ctagaaggca cagtcgaggc tgatcagcga gctctaggat ctgcattcca ccactgctcc
     3181 cattcatcag ttccataggt tggaatctaa aatacacaaa caattagaat cagtagttta
     3241 acacattata cacttaaaaa ttttatattt accttagagc tttaaatctc tgtaggtagt
     3301 ttgtccaatt atgtcacacc acagaagtaa ggttccttca caaagagatc gcctgacacg
     3361 atttcctgca caggcttgag ccatatactc atacatcgca tcttggccac gttttccacg
     3421 ggtttcaaaa ttaatctcaa gttctacgct taacgctttc gcctgttccc agttattaat
     3481 atattcaacg ctagaactcc cctcagcgaa gggaaggctg agcactacac gcgaagcacc
     3541 atcaccgaac cttttgataa actcttccgt tccgacttgc tccatcaacg gttcagtgag
     3601 acttaaacct aactctttct taatagtttc ggcattatcc acttttagtg cgagaacctt
     3661 cgtcagtcct ggatacgtca ctttgaccac gcctccagct tttccagaga gcgggttttc
     3721 attatctaca gagtatcccg cagcgtcgta tttattgtcg gtactataaa accctttcca
     3781 atcatcgtca taatttcctt gtgtaccaga ttttggcttt tgtatacctt tttgaatgga
     3841 atctacataa ccaggtttag tcccgtggta cgaagaaaag ttttccatca caaaagattt
     3901 agaagaatca acaacatcat caggatccat ggcgaggacc tgcaggtcga aaggcccgga
     3961 gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca gaatgccggg
     4021 cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag cccgcccccg
     4081 gacccacccc ttcccagcct ctgagcccag aaagcgaagg agcaaagctg ctattggccg
     4141 ctgccccaaa ggcctacccg cttccattgc tcagcggtgc tgtccatctg cacgagacta
     4201 gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg ggcggggggg
     4261 aacttcctga ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa gaacggagcc
     4321 ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc agaggccact tgtgtagcgc
     4381 caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa aagcgcctcc
     4441 cctacccggt agaattcgat atcaagctta tcgataccgt cgacggtatc gataagctag
     4501 atgtcctgaa atatttctat cagaacaagg tagtataaag ctggtaggta tacaaaacgc
     4561 tagactagtt tctatccctg acccttaatc tgctagtata tccgtaggaa gttgcttaag
     4621 tgccactagt accaacagcc tctgttagag tgaacagtag attttaatga agggccaata
     4681 aagaccactt aagactactg actgaattaa aaatccaccc agtggagtta gtattggtta
     4741 tagttatata acaaaatatc caacttagcc aggcgtggtg gtgcacgcct ttaatcccgg
     4801 cactcaggag gcagaggcag gcggatttct gagttcgagg ccagcctggt ctacaaagtg
     4861 agttccagga cagccagggc tatacagaga aaccctgtct cgaaaaacca aaaaaaaaaa
     4921 aaaaaaggaa aaaaaatatg gcttgaaaac atgctattgt tgtcaaaagg agatccaaac
     4981 aagtatttct ttgaacaaaa attatgtaga aaacagggtg ataaagaaca agggcttttt
     5041 aaaaaaggcc agcattattt gagatataca agtgactcca attcaagcct aaaggccact
     5101 caatgctcac taacagtgct aatcagaatc aattccttag cccagtcaaa aatagtcctg
     5161 aatatcttct ggataaacat gtcctccaac atataaacta gtatacacta acaaaacgtc
     5221 tcaacttcaa ggtgaaatgc ttgactccta gacttgtgac ccagcaaagt gctctatagg
     5281 tagggttact aggtcagaga gtcttgcctg caaaccacaa ttatacaatt ccaccaaatg
     5341 taagacatgt aaaaatgact tatttagctc aggttttgaa aattaaaaat caaacccaca
     5401 aagacctaac tttcacatta agtacaaatg tttaacatat ataacatgtt ttgccttgat
     5461 atatgaaatc atatgacatc acctgacagg aaacttaagt ttatttgaac aacaatcagc
     5521 ctaaggtagg cctagcacat gatcaggata gtgcagggaa acccaaagaa gtgcttctga
     5581 gtataaacca agagacctcg aaatagcagc tttgttctgt atctcatggg ctttaactat
     5641 cacttgaaaa caatttcaca aatcacaaaa ccaacatttg tttagtttcc aactctgcct
     5701 attcaccgga gaaatccatg tcccaatttg atgggggaaa acttgaatga aatattattt
     5761 ggaatatttt aatatccctg tgtatatgca gatggtttaa agacaggtaa ccttaactgt
     5821 agtttcttgg ttgtctgcct tgttttttga aatatgctct caccaggagc ctgccaagta
     5881 actactcttg tgtgctcagc aagtcctagg gatcccttaa gcatgctcta acaggcctgg
     5941 cttttttttt taaaaagtag gttgcggtga taataattca agttttcatc acttgaaaca
     6001 cattttacca actatcaccc aagctccctt agtgtgtccc ctataaaaga atctgacctg
     6061 caagttccaa aagtattact acattttaac cttagtattc aggagacaaa agcaggcaga
     6121 actttagtct acatatcttg ttgcagatca ggcagagata catagtgaga ccctgcctca
     6181 ggaaaagcta tcatttttat attcacagta agcaaattaa cattaaaagt cagaaattct
     6241 taatttgact ttggctgtga agaatttgga ttctctggat ttgaaactag aaaatctgat
     6301 taagcgatgc aacagtttct gtatactaaa actctaagac ccttggttct aaagatacca
     6361 catttaaaaa aaaagttaag tataagacaa aaagccttaa tagctctttt ataggggagg
     6421 gactcattta atattagtcc acctcactcc tcataactat tttatgaggt ttattatata
     6481 acccattttt taaaaaaaaa attaaaggac aattttagtg tttgaaagat ttcccaaccc
     6541 caccctggaa atcaggctgc aaatctcagc agctgccctt agaaggcaaa aagccatgcc
     6601 atttaagcca tgggaagtta gtagcaaaca agagaccaca tggcaggaag gaggctttaa
     6661 agaaagccca cagtgtcaaa agacccaacc aacagcagag acagaaaaaa taatacccaa
     6721 atctcatcag aaaggtagac ggatttagcc acatccatag tggctcatta gggaatgctt
     6781 caattataat tttatgaaat ctcagagccc ccctccccaa cagggtttct gtgtgtattc
     6841 ctggctatcc tagatagaac ttgatgtgta gaccaggctg ggctcaaact cagagatctg
     6901 cctgtctctg cctccagagt gctgggatta aaggcataaa ggcaagcacc accactggct
     6961 ggctaaactc tggccctaca tttttgaaat ccaaaattat cattacttgc tttcatgttt
     7021 agtaatggct gcagacttag ctttcagctt tgtatagatg aagcacaaaa gttaagtaaa
     7081 gctagcagtt taaacaaata ggatactaga agttcaattt ttatttcttt attgcttagt
     7141 gtaagagaaa agacattaaa gtctgcattg gtcttctgta tcccacaagt ctgcagttat
     7201 ggctcctctg tccacagtta cacttcaaat aaaaattagt tccttggtaa catttccagt
     7261 gcttaaagag taatttgatt attggctacc atattggaac aaacacaaag tatttcatta
     7321 cttcagtaaa ttctactgga gacagtattc tctttaaaaa aaaaaaaaag aagaagaagg
     7381 aggatttatt tatttaatgt gaatacactt gtggtcttca gacacaccag aagagggcat
     7441 cagatcccat tacagatggt tgtgagccac catgtggttg ctgggaattg aactcaggac
     7501 ttctggaaga gcagtctgtg ctcttgaact ccgagccacc tctccagccc ctggatagta
     7561 ttattataga gcacaagcac acacaacaca acaacctaaa aaccaagctt cacaaataca
     7621 tttgttgctt aatcatttta cttgtgtttg gctacaagtc aagcaaaatt ataggtcctg
     7681 aagaagcttg gcaaaatcac atttagacca gcaataacgt gtagaatgcc atgagtcaag
     7741 ccagtccaag agaaagcact gcttaaatag aaaaaataca agctcacaag accttaggtc
     7801 aggaaagaca acaaacacct gaactttgca ttccaaaagg aaccaccttt tacagatgtg
     7861 tacaatgaca aaattttagt aagcagtaat caataccatg tggctcaata atgaaatata
     7921 ccttttaatg tcttaaaata tcagcctggc aatatgtaag atacatcagg taatattggg
     7981 ggaggagaca tccacctgga aaccattaat ggttaatagc tcagtttata aatggagaaa
     8041 aaggagagag gcattcatgg gagtggaaag ttaagcttta ggttggattc tcaatacatc
     8101 tattgttgta aaactccctg gactgagaat aggcccaaat gtggaacacc acctgacggg
     8161 agaggtgata gacactgaaa attaaggatc aaggcaaagg atcaacaaaa agtgtactaa
     8221 ggagttataa aagaactgca gtgttgaggc cccagctaca gcctcgattt gtggtgtatg
     8281 taactaatct gtctggtttc atgagtcatc agacttctaa gatcaggaaa gggaaaatgc
     8341 caatgctctg tctaggggtt ggataagcca gtataataaa tgaaaatggg gctaaaatga
     8401 gtgttctaaa ataccttttg ataaggctgc agaaggagcg ggagaaatgg atatgaagta
     8461 ctgggctctt taaaaatgat taaaattctg cttacatagt ctaactcgcg acactgtaat
     8521 ttcatactgt agtaaggatc tcaagcagga gagtataaaa ctcgggtgag catgtcttta
     8581 atctacctcg atggaaaata ctccgaggcg gatcacaagc aataataacc tgtagttttg
     8641 ctgcataaaa ccccagatga ctacctatcc tcccattttc cttatttgcc cctattaaaa
     8701 aacttcccga caaaaccgaa aatctgtggg aagtcttgtc cctccaattt tacacctgtt
     8761 caattcccct gcaggacaac gcccacacac caggttagcc tttaagcctg cccagaagac
     8821 tcccgcccat cttctagccg gcgcgccaac tagaggatcg agccccagct ggttctttcc
     8881 gcctcagaag ccatagagcc caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc
     8941 tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa
     9001 tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc
     9061 aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag
     9121 gctgatcagc gagctcgcgg ccgcccgcgg gggcccatcg aaccactttg tacaagaaag
     9181 ctgggtctag attaatgcgc actacgcccc caactgagag aactcaaagg ttaccccagt
     9241 tggggcacta ccagatatca tcgtcgacat tgattattga ctagttatta atagtaatca
     9301 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     9361 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     9421 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     9481 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     9541 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     9601 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     9661 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     9721 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggsgcg cgccrggsgg
     9781 gggggggggg ggsgrggggs ggggsggggs grggsggrgr ggkgcggcgg cagccaatca
     9841 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     9901 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc
     9961 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
    10021 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
    10081 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
    10141 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
    10201 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
    10261 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg cgaggggaac
    10321 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc
    10381 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt
    10441 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag
    10501 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaggggcgc
    10561 ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta
    10621 tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa
    10681 tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc
    10741 aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct
    10801 ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg
    10861 gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc
    10921 ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt
    10981 ggcaaagaat tcctcgaggc cgccaccatg gtgagcgagc tgattaagga gaacatgcac
    11041 atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc
    11101 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggcggtcga gggcggccct
    11161 ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa aaccttcatc
    11221 aaccacaccc agggcatccc cgacttcttt aagcagtcct tccccgaggg cttcacatgg
    11281 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
    11341 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc
    11401 cctgtgatgc agaagaaaac actcggctgg gaggcctcca ccgagaccct gtaccccgct
    11461 gacggcggcc tggaaggcag agccgacatg gccctgaagc tcgtgggcgg gggccacctg
    11521 atctgcaact tgaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc
    11581 ggcgtctact atgtggacag aagactggaa agaatcaagg aggccgacaa agagacctac
    11641 gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac
    11701 agaggcgcca caggtagtcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag
    11761 gtagatccaa aaaagaagag aaaggtatta taagccgcac gaccccatgc atcgatgatc
    11821 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
    11881 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
    11941 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
    12001 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
    12061 ctctatgggc tcgataagct tgatatccat taagggttcc ggatcagctt gatggggatc
    12121 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
    12181 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
    12241 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
    12301 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc
    12361 tagagtcgca gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
    12421 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
    12481 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
    12541 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
    12601 tgattatgat cctctagagt cgcagatcca gacatgataa gatacattga tgagtttgga
    12661 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
    12721 gctttatttg taaccattat aagctgcaat aaacaatcag aagaactcgt caagaaggcg
    12781 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
    12841 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
    12901 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
    12961 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcatcgc cgtcgggcat
    13021 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
    13081 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
    13141 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
    13201 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
    13261 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
    13321 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
    13381 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
    13441 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
    13501 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatgg ccgatcccat
    13561 ggtggcggcg tgctacgccc ccaactgaga gaactcaaag gttaccccag ttggggcact
    13621 acagatctcc cgatccgtcg ccagcctgct tttttgtaca aacttgtttg atgaggtcga
    13681 cggtatcgat aagctagctt gggctgcagg tcgagggacc taataacttc gtatagcata
    13741 cattatacga agttatatta agggttccgg atccactagt tctagagcgg cctcgactct
    13801 acgataccgt cgatccccac tggaaagacc gcgaagagtt tgtcctcaac cgcgagctgt
    13861 ggaaaaaaaa gggacaggat aagtatgaca tcatcaagga aaccctggac tactgcgccc
    13921 tacagatccc ttaattaagg ctagaaagac tggagttgca gatcacgagg gaagaggggg
    13981 aagggattct cccaggccca gggcggtcct cagaagccag gaggcagcag agaactccca
    14041 gaaaggtatt gcaacactcc cctcccccct ccggagaagg gtgcggcctt ctccccgcct
    14101 actccactgc agctccctta ctgataacaa ctcagagcga ctttgggaga gcaagtgctt
    14161 cctgcctcca aaacagccca actgagccct cgctccttcc ctccactccc cggagtgcgc
    14221 gatggaggtc tggctcagca cgcccctctt gaggcaactc aagtcggaaa cgtgcttgca
    14281 cccgccccgc agccgctcag ccctactgcc cgtccccgcc cccagcgcgc gcttcctgcc
    14341 acgttgcgca ggggcgcgcg gccagactct gcggcgcggg gccgagggga gggccggaac
    14401 ctgggagcgc ctcctcgccg cccccgctgg ccggcggatg gactcaactt gcacgaacac
    14461 gagccaatgg caagggccag ttttctgggc cccgagagcc aatcagacga cgaggcccgg
    14521 ccggcggcgg gtaaaacgac tcccccagag gaaggggagg gtgggcggcc gctcgcgcgc
    14581 gagctacttt cgctgaccct ccctccccyc ccccgccccg ccagaggccg accgcgcccg
    14641 cacgtccagc tcgcctcacc ccacctacct cccgccccac ccagtgggca gagcgaggct
    14701 gccggcggct gcgcactccg gctgccgtta actgacaggc gccttacgcc aaccaaaaca
    14761 cgccatttgt gttttcacac acggcgggag gaaaagaagc caatcagcga cgagacgtcg
    14821 gccggaagcg ctcctccgct gccccccccc ccccgagcca tggccgcgtc cggtggagac
    14881 ttttccgctc ccttctccct ccccctccgg ttgctgcagg gcggaccgca ttcctgccca
    14941 ccacccgctt gccccttcca gcgtcacgac tcgtacccgg ctgtctcaca gaacggctcc
    15001 accacgctcg gagggcctgc cgcggtg
//
