LOCUS       sequence-361611-        9784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR 
            insertion, Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 9784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Hygro DEST (w17-1)
FEATURES             Location/Qualifiers
     source          1..9784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(106..174,175..966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(324..343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1879..1896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2013..2034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2049..2054,2055..2072,2073..2079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2087..2103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2092..2114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2111..2127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2111..2127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2146..2166
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2148..2166
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2194..2420
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2421..2601
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2648..2773
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3266..3499
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3684..3728
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3995..4111
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4167..4755
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4220..4240)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4638..4649)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4811..5310
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4846..4869)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5243..5262
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5328..6353
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5328..6353
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    6770..6894
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6935..7240)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(7037..7056)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(7582..8241)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8156..8175
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(8295..8301,8302..8319,8320..8325))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(8350..8474)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             8549..8729
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8801..8922
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8838..8857)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8892..8911
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8962..9097
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9117..9136)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9118..9136)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9146..9163)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9146..9162)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9155..9177)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9304..9759
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9391..9410)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9601..9622
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     1921 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     1981 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2041 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2101 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     2161 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     2221 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     2281 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     2341 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     2401 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     2461 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2521 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2581 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     2641 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     2701 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     2761 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     2821 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     2881 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     2941 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     3001 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     3061 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     3121 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     3181 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     3241 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     3301 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     3361 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     3421 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     3481 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     3541 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     3601 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     3661 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     3721 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     3781 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     3841 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     3901 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     3961 gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg
     4021 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4081 tacaaaaaca aattacaaaa ttcaaaattt tatcgtccag tgtggtggaa ttctgcagat
     4141 atccagcaca gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     4201 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4261 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4321 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4381 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4441 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     4501 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     4561 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     4621 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     4681 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     4741 gggccgcctc cccgcctgga attctgcaga tatccggact agtgatctct cgaggttaac
     4801 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     4861 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     4921 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     4981 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     5041 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     5101 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     5161 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     5221 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     5281 ttctcctctt cctcatctcc gggcctttcg acctgcatcc cgccaccatg aaaaagcctg
     5341 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
     5401 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     5461 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     5521 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
     5581 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
     5641 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
     5701 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     5761 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     5821 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     5881 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     5941 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     6001 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     6061 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     6121 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     6181 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     6241 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
     6301 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagagtagat
     6361 gccgaccgaa caagagctga tttcgagaac gcctcagcca gcaactcgcg cgagcctagc
     6421 aaggcaaatg cgagagaacg gccttacgct tggtggcaca gttctcgtcc acagttcgct
     6481 aagctcgctc ggctgggtcg cgggagggcc ggtcgcagtg attcaggccc ttctggattg
     6541 tgttggtccc cagggcacga ttgtcatgcc cacgcactcg ggtgatctga ctgatcccgc
     6601 agattggaga tcgccgcccg tgcctgccga ttgggtgcag atccgtcgag ggcccggtac
     6661 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6721 ggggactgga agggctagac tcgagcggcc gccactgtgc tggatatcaa ccactttgta
     6781 caagaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     6841 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggtcgac
     6901 ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac atcaggttaa
     6961 tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg
     7021 agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca
     7081 ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga
     7141 tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat
     7201 aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc cctgttctcg
     7261 tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt
     7321 tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc
     7381 agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg
     7441 tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg tatacatatc
     7501 agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt atctggcttt
     7561 tagtaagccg gatccagatc tttacgcccc gccctgccac tcatcgcagt actgttgtaa
     7621 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     7681 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     7741 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     7801 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     7861 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     7921 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     7981 actatcccat atcaccagct caccgtcttt cattgccata cggaattccg gatgagcatt
     8041 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     8101 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     8161 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     8221 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg acggatccta
     8281 actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8341 tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     8401 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttt
     8461 ttgtacaaac ttgttgatat ctgcagaatt ccaccacact ggactagctc actcccaacg
     8521 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     8581 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8641 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8701 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     8761 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     8821 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8881 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     8941 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9001 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9061 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     9121 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     9181 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     9241 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     9301 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     9361 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     9421 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     9481 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     9541 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     9601 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     9661 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     9721 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     9781 tgtg
//
