LOCUS       sequence-361618-       10071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-off genome insertion system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10071)
  AUTHORS   Lemberg C, Martinez de San Vicente K, Frois-Martins R, Altmeier S, 
            Tran VDT, Mertens S, Amorim-Vaz S, Rai LS, d'Enfert C, Pagni M, 
            Sanglard D, LeibundGut-Landmann S
  TITLE     Candida albicans commensalism in the oral mucosa is favoured by 
            limited virulence and metabolic adaptation.
  JOURNAL   PLoS Pathog. 2022 Apr 11;18(4):e1010012. doi: 
            10.1371/journal.ppat.1010012. eCollection 2022 Apr.
  PUBMED    35404986
REFERENCE   2  (bases 1 to 10071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2022-04-11"; volume: "18(4):e1010012. doi"; pages: 
            " 10.1371/journal.ppat.1010012. eCollection 2022 Apr"
COMMENT     Sequence Label: pDS2142
FEATURES             Location/Qualifiers
     source          1..10071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2114..3139
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(5545..5565)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5545..5563)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5584..5600)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5584..5600)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5597..5619)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5608..5624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5632..5638,5639..5656,5657..5662))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5677..5698
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5815..5832)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5986..6574)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6066..6085)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6745..7536,7537..7605))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7368..7387
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7606..7710)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7736..8191)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7873..7894)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8085..8104
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8318..8340
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8332..8349
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8333..8349
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8359..8378
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8359..8377
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgttaaacga agttggtatt gaaggtttga caacaagaaa attagcacaa aagttgggtg
       61 ttgaacaacc aacattgtac tggcacgtta agaataaaag agctttgttg gatgctttgg
      121 caattgaaat gttagataga catcatactc acttctgtcc attggaaggt gaatcatggc
      181 aagatttctt aagaaacaac gctaaatctt tcagatgtgc attattatca cacagagatg
      241 gtgcaaaggt tcatttaggt actagaccaa cagaaaaaca atatgaaaca ttggaaaacc
      301 aattggcatt cttatgtcaa caaggttttt cattagaaaa tgctttatat gcattgtctg
      361 ctgttggtca ttttacatta ggttgtgttt tagaagatca agaacatcaa gttgcaaagg
      421 aagaaagaga aacacctact actgattcaa tgcctccatt gttaagacaa gctattgaat
      481 tattcgatca tcaaggtgct gaacctgctt ttttatttgg tttagaatta attatttgtg
      541 gtttagaaaa acaattaaaa tgtgaatctg gttcatctac tttattcggt ggtgctaatt
      601 ttaaccaatc aggtaacatt gctgattcat cattatcatt tacttttact aactcatcaa
      661 atggtcctaa tttaattact actcaaacta attcacaagc tttatcacaa ccaattgctt
      721 catcaaatgt tcatgataat tttatgaata atgaaattac tgcttcaaaa attgatgatg
      781 gtaataattc aaaaccatca tcaccaggtt ggactgatca aactgcttat aacgcgtttg
      841 gaatcactac agggatgttt aataccacta caatggatga tgtatataac tatctattcg
      901 atgatgaaga taccccacca aacccaaaaa aagagtaagg atctaaattc tggaaatctg
      961 gaaatctggt tttgtattct tgttattctt ctttttgtta ttacatatat aacttgttac
     1021 ttttttaaaa aaatctttgt ttattttata aatatataaa actaaattta agaaaaagag
     1081 aaaaatgttt tatttgagag attgaaattt tacttgaatt tagcttagct tttataaagt
     1141 attattatgt aaaaaaacaa aacaaatata cattaaaaag ttaagactat aaaatagcca
     1201 cccaaggcat ttctatatct tgttgttgtt gttttcatct tctgtatcag aggaacttat
     1261 tttattattt tcgtcacggg tattttctct tgtttgatga ttcatcccat tccttccatc
     1321 ataaaatgtc tgcaggacca cctttgattg taaatagtaa taattaccac ccttatctaa
     1381 ttatttattt aacttattta tttatttatt atacatatat acaaatctaa taaagtgaaa
     1441 atctccccct tcacacttca catatgtata gtgcttgctg ttcgatattg ctagaggcaa
     1501 agaaaaagga ttacctcacg cctaaaatgg aatttgaaaa ttgaggagat gagttgagct
     1561 tttgtctttt acactttccc tattcaattg tgccgcaaaa acttcaatat ctaacacagt
     1621 gtgaaatatt tgttatgtat accgaaagcc aaataccatt taaactggta tttcaagatt
     1681 tcataaatca agtttctggt taaattaatt gctgttaaac agaacaagag cttcacattg
     1741 atgttggaat aactactata gaattataaa atacacaaaa ccaggtcgtg tacgcaataa
     1801 cttttcaatg tatgaaaaaa tttttttttt tttttttgct tcttctcttc gcttcttttt
     1861 tctagttatc aatttggcaa tgaattgatt tgattgccct ttgtttagtt tactatgaaa
     1921 aaaaattttt tttttgtctc tgtcagattt tctctcattt ttttttttgc ctcataatga
     1981 acaactcaaa aaagaatata aatttctcat caaagacctt tccaattgaa aattttttct
     2041 ttccttcttt tccttttttt ttttctttct ttcttctttt ctaagtatac attttatata
     2101 atcaatcctc gagatgaaaa aaccagaatt gactgctact tctgttgaaa aatttttgat
     2161 tgaaaaattt gattctgttt ctgatttgat gcaattgtct gaaggtgaag aatcaagagc
     2221 tttttctttt gatgttggtg gtagaggtta tgttttgaga gttaattctt gtgctgatgg
     2281 tttttataaa gatagatatg tttatagaca ttttgcttct gctgctttgc caattccaga
     2341 agttttggat attggtgaat tttctgaatc tttgacttat tgtatttcaa gaagagctca
     2401 aggtgttact ttgcaagatt tgccagaaac tgaattgcca gctgttttgc aaccagttgc
     2461 tgaagctatg gatgctattg ctgctgctga tttgtctcaa acttctggtt ttggtccatt
     2521 tggtccacaa ggtattggtc aatatactac ttggagagat tttatttgtg ctattgctga
     2581 tccacatgtt tatcattggc aaactgttat ggatgatact gtttctgctt ctgttgctca
     2641 agcattggat gaattgatgt tgtgggctga agattgtcca gaagttagac atttggttca
     2701 tgctgatttt ggttctaata atgttttgac tgataatggt agaattactg ctgttattga
     2761 ttggtctgaa gctatgtttg gtgattctca atatgaagtt gctaatattt ttttttggag
     2821 accatggttg gcatgtatgg aacaacaaac tagatatttt gaaagaagac atccagaatt
     2881 ggctggttct ccaagattga gagcttatat gttgagaatt ggtttggatc aattgtatca
     2941 atctttggtt gatggtaatt ttgatgatgc tgcttgggct caaggtagat gtgatgctat
     3001 tgttagatca ggtgctggta ctgttggtag aactcaaatt gctagaagat cagctgctgt
     3061 ttggactgat ggttgtgttg aagttttggc tgattctggt aatagaagac catctactag
     3121 accaagagct aaagaataag gatccgagtg aaattctgga aatctggaaa tctggttttg
     3181 tattcttgtt attcttcttt ttgttattac atatataact tgttactttt ttaaaaaaat
     3241 ctttgtttat tttataaata tataaaacta aatttaagaa aaagagaaaa atgttttatt
     3301 tgagagattg aaattttact tgaatttagc ttagctttta taaagtatta ttatgtaaaa
     3361 aaacaaaaca aatatacatt aaaaagttaa gactataaaa tagccaccca aggcatttct
     3421 atatcttgtt gttgttgttt tcatcttctg tatcagagga acttatttta ttattttcgt
     3481 cacgggtatt ttctcttgtt tgatgattca tcccattctt tccatcataa aatcatatgg
     3541 cgctgagaac cgttatcgat aactaaagca gcaacttctt ctataaaaat gggttagtat
     3601 gacagtcatt taaataagga atttttcagt tggcttggtt tcaattcaat gttcgttttt
     3661 ttttttcttg ctgtgtttgt gtttgtgttg tttatagttg tgtgcactga tcgtcgaaaa
     3721 aaaaaattca tagtgagccg ggaaatctgt atagcccaga taacaacaca agtccaaact
     3781 agaaactcgt caaacaccaa aagcaatgtt gaatcaattg ccttgcacaa gtacacgtag
     3841 gaaaacataa aacattgcaa ttttgaatat tgagcctttt gtcgtaacat tgattgatag
     3901 gattactcac cgaatggttt tgaaaccact gccgacagat caatcaatca atcaaaaaac
     3961 gtgaactttg aaaaagggga agaacagata cattgaagtt agccatttcc actgatcgtc
     4021 acaacatatc tgataaatta ctttcaaaat tataagctga tgtgtgtgta ttattaatgt
     4081 gacagtaaca tcccaaacga gaaatattat ctcgacaaca aaaaagtttg atctgaattg
     4141 aaaatgaagt tttcccaccc tacccatttg tcatattgaa accaatcaac tgattaatca
     4201 atcaattaga attgaagcta aactaaaaca taccaccgtc cattttgaat gattatattt
     4261 ttttaatatt aatatcgaga taatgtttct aagaaagaaa gaaaaccagg agtgaaaatt
     4321 agaaaaggaa aggaaaggaa aaaaagaaaa atctgaaaat atataaaaaa aaattgtttc
     4381 gttggcaata aatcttggtg agaacagcga ccgaaagcaa ataagaacaa aatatgagtg
     4441 tattacgttg aacaactaat taacgtgtgt gtatggatct ttttttcttt tttctcttta
     4501 accgactata aacaacaaac atttttgggc agtgcacaca ctacttaata tacacagcat
     4561 aaattacacg attagaaaca aattagctta ttaaaataac ctaatcaaac cgaatatttt
     4621 atggtattat gagtaaacta tataatataa atagcacaca cccacaacaa caacaaagga
     4681 aaactaaaag gttttttctt tttgaaaaga tcgttttctt tattattctc tagttttgac
     4741 gctcgacatt ttatgatgga atgaatggga tgaatcatca aacaagagaa aatacccgtg
     4801 acgaaaataa taaaataagt tcctctgata cagaagatga aaacaacaac aacaagatat
     4861 agaaatgcct tgggtggcta ttttatagtc ttaacttttt aatgtatatt tgttttgttt
     4921 ttttacataa taatacttta taaaagctaa gctaaattca agtaaaattt caatctctca
     4981 aataaaacat ttttctcttt ttcttaaatt tagttttata tatttataaa ataaacaaag
     5041 atttttttaa aaaagtaaca agttatatat gtaataacaa aaagaagaat aacaagaata
     5101 caaaaccaga tttccagatt tccagaattt agatccatgg gtttgagagt tgtcgctatt
     5161 gacggtggtg acgaaaaagg tgaatttgtt aaatcattgg gtgctgaagc ttacgttgat
     5221 ttcaccaaag ataaagatat tgttgaagct gtcaagaaag ctactgatgg tggtccacac
     5281 ggtgctatca atgtctctgt ctcagaaaga gccattggtc aatccactga atatgtcaga
     5341 actttaggta aagttgtttt ggttggtttg ccagctggta ctaaaattag taccccagtc
     5401 tttgatgctg ttatcaagac cattcaaatt aaaggttctt atgtcggtaa cagaaaagat
     5461 actgctgaag ccgttgattt cttcacaaga ggtttgatca aatgcccaat caagattgtc
     5521 gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     5581 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     5641 agccgggagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     5701 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5761 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     5821 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5881 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5941 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6001 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6061 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6121 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6181 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6241 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6301 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6361 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6421 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6481 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6541 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6601 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6661 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6721 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6781 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6841 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6901 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6961 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7021 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7081 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7141 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7201 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7261 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7321 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7381 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7441 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7501 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7561 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7621 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7681 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     7741 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     7801 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     7861 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     7921 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     7981 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     8041 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     8101 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     8161 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
     8221 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     8281 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     8341 acggccagtg agcgcgcgta atacgactca ctatagggcg aattggagct ccaccgcggt
     8401 tgagatggag ccgtcaaata tccaaaacac tcttctttca actaatttgc cgtaaactat
     8461 ctccatcgta cgattaagat aaaaaaaaaa caattctttc ttacaatatt tgatagagac
     8521 ccaatgcaaa gccagcgaga aagaggagta ttggcattgt tgggtagtag atgaatgagt
     8581 aatcttctag ccaaaacaaa ataaaccaca agaacagtag gcaaaattga gtaattgata
     8641 gcgcttgtag tctttgtatt taaaaaaacc taattgtcca aaaattgaaa atcatgagtt
     8701 gcaaatttgt tgaaatcacc aggtaaatag atcatttagc tatatcgcct tatcattctt
     8761 cgtacaagaa ttatacacaa ataccaccac cacaccaccc agaattttta cacgtgtttt
     8821 ctaaattatt actttccgtg gccaatcaaa ttgaaatttt tcttgttcga aacggcagtg
     8881 cctcaaacat tatagtagca agtattgcct aaagagtttc cgtctcgtgc ccccccccta
     8941 tccccttttg taatttgcca aaatagctca aaaaggataa tcccgcactt tttttttccg
     9001 agttctcgtc atgcaagcaa gcttattcag aattttcaga ggtgcgagtc tcaccactac
     9061 cactgcagct gcatctttta cagcaacagc aacagcaggt gccaccacgg caaagacatt
     9121 gtctggatct cgagcacaca catacagaca attgcgatat tttattttag ggtcaatttg
     9181 gaacgtcacc atacagtatt cagtatgatt aatttgactt gcaaaataca aatcgcgtgg
     9241 ttcaattccg aaaaattgat agtgttccat gaaaaattac aaggagagcc gtgaattata
     9301 acaaatttac tcacattttg gctggcaatt tctttaaccc ccttaatttt tttcatgcag
     9361 tttattattt tacgtagaag cttattctct aaacactcgg ttatccgaat atattttgct
     9421 ttcagagcaa ttgactgata taataccatc aattgaataa atcagtatca tcaaatgcta
     9481 gtttgagttc aataaaaggt ataatcggtg ttaactgaaa ggtattgtct aattaattag
     9541 gttgcggttt ttggatcagc tgtttcaaat ccaggctcgt ggtgcacatt ttttattttt
     9601 gttgtgatga ttttttcttt tctttgctta atagttagtt agtaaaatgg ataatgacgc
     9661 aaaattgctt ctgaatggat gataatgccc cgggtatggc gtgtcccgta caaatcatat
     9721 tatgcaaaaa tagccaaaat agacaaaaat aggtggaaat tgtttcaacc aacccactca
     9781 accactaaaa tttgtcttgt caaagccaat tccaacgaaa attggatttt ttttgactta
     9841 atatatatta acccttgaaa ttcccttcaa ttggaaaatt tttttaattt atttatttct
     9901 tctttctttt tttctttttc tttttctttc ttttattcat cacaattgaa ttcaaatcaa
     9961 ttaacatcaa caaactttac aatcgttatt gtcatacaac aacaacaaca aatacaaaaa
    10021 caattatgtc tagattggat aaatctaaag ttattaattc tgctttggaa t
//
