LOCUS       sequence-361619-       10071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-on genome insertion system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10071)
  TITLE     Tet-on gene expression system for Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDS2149
FEATURES             Location/Qualifiers
     source          1..10071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             930..1550
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1003..1022)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             1569..1913
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             3089..4114
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(6520..6540)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6520..6538)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6559..6575)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6559..6575)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6572..6594)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6583..6599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6607..6613,6614..6631,6632..6637))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6652..6673
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6790..6807)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6961..7549)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7041..7060)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7720..8511,8512..8580))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8343..8362
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8581..8685)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8711..9166)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8848..8869)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9060..9079
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9293..9315
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9307..9324
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9308..9324
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9334..9353
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9334..9352
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aggtgccacc acggcaaaga cattgtctgg atctcgagca cacacataca gacaattgcg
       61 atattttatt ttagggtcaa tttggaacgt caccatacag tattcagtat gattaatttg
      121 acttgcaaaa tacaaatcgc gtggttcaat tccgaaaaat tgatagtgtt ccatgaaaaa
      181 ttacaaggag agccgtgaat tataacaaat ttactcacat tttggctggc aatttcttta
      241 acccccttaa tttttttcat gcagtttatt attttacgta gaagcttatt ctctaaacac
      301 tcggttatcc gaatatattt tgctttcaga gcaattgact gatataatac catcaattga
      361 ataaatcagt atcatcaaat gctagtttga gttcaataaa aggtataatc ggtgttaact
      421 gaaaggtatt gtctaattaa ttaggttgcg gtttttggat cagctgtttc aaatccaggc
      481 tcgtggtgca cattttttat ttttgttgtg atgatttttt cttttctttg cttaatagtt
      541 agttagtaaa atggataatg acgcaaaatt gcttctgaat ggatgataat gccccgggta
      601 tggcgtgtcc cgtacaaatc atattatgca aaaatagcca aaatagacaa aaataggtgg
      661 aaattgtttc aaccaaccca ctcaaccact aaaatttgtc ttgtcaaagc caattccaac
      721 gaaaattgga ttttttttga cttaatatat attaaccctt gaaattccct tcaattggaa
      781 aattttttta atttatttat ttcttctttc tttttttctt tttctttttc tttcttttat
      841 tcatcacaat tgaattcaaa tcaattaaca tcaacaaact ttacaatcgt tattgtcata
      901 caacaacaac aacaaataca aaaacaatta tgtctagatt agataaaagt aaagtgatta
      961 acggcgcatt agagttgctt aatggggtcg gaatcgaagg tttaacaacc cgtaaactcg
     1021 cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt
     1081 tgctcgacgc cttaccaatt gagatgttag ataggcacca tactcacttt tgccctttag
     1141 aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac
     1201 taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg
     1261 aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat
     1321 tatatgcact cagcgcagtg gggcatttta ctttaggttg cgtattggaa gaacaagagc
     1381 atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac
     1441 gacaagctat cgaattattt gatagacaag gtgcagagcc agccttctta ttcggccttg
     1501 aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc tcgactttgt
     1561 ttggtggcgc caattttaat caaagtggga atattgctga tagctcattg tccttcactt
     1621 tcactaacag tagcaacggt ccgaacctca taacaactca aacaaattct caagcgcttt
     1681 cacaaccaat tgcctcctct aacgttcatg ataacttcat gaataatgaa atcacggcta
     1741 gtaaaattga tgatggtaat aattcaaaac cattgtcacc tggttggacg gaccaaactg
     1801 cgtataacgc gtttggaatc actacaggga tgtttaatac cactacaatg gatgatgtat
     1861 ataactatct attcgatgat gaagataccc caccaaaccc aaaaaaagag taaggatcta
     1921 aattctggaa atctggaaat ctggttttgt attcttgtta ttcttctttt tgttattaca
     1981 tatataactt gttacttttt taaaaaaatc tttgtttatt ttataaatat ataaaactaa
     2041 atttaagaaa aagagaaaaa tgttttattt gagagattga aattttactt gaatttagct
     2101 tagcttttat aaagtattat tatgtaaaaa aacaaaacaa atatacatta aaaagttaag
     2161 actataaaat agccacccaa ggcatttcta tatcttgttg ttgttgtttt catcttctgt
     2221 atcagaggaa cttattttat tattttcgtc acgggtattt tctcttgttt gatgattcat
     2281 cccattcctt ccatcataaa atgtctgcag gaccaccttt gattgtaaat agtaataatt
     2341 accaccctta tctaattatt tatttaactt atttatttat ttattataca tatatacaaa
     2401 tctaataaag tgaaaatctc ccccttcaca cttcacatat gtatagtgct tgctgttcga
     2461 tattgctaga ggcaaagaaa aaggattacc tcacgcctaa aatggaattt gaaaattgag
     2521 gagatgagtt gagcttttgt cttttacact ttccctattc aattgtgccg caaaaacttc
     2581 aatatctaac acagtgtgaa atatttgtta tgtataccga aagccaaata ccatttaaac
     2641 tggtatttca agatttcata aatcaagttt ctggttaaat taattgctgt taaacagaac
     2701 aagagcttca cattgatgtt ggaataacta ctatagaatt ataaaataca caaaaccagg
     2761 tcgtgtacgc aataactttt caatgtatga aaaaattttt tttttttttt ttgcttcttc
     2821 tcttcgcttc ttttttctag ttatcaattt ggcaatgaat tgatttgatt gccctttgtt
     2881 tagtttacta tgaaaaaaaa tttttttttt gtctctgtca gattttctct catttttttt
     2941 tttgcctcat aatgaacaac tcaaaaaaga atataaattt ctcatcaaag acctttccaa
     3001 ttgaaaattt tttctttcct tcttttcctt tttttttttc tttctttctt cttttctaag
     3061 tatacatttt atataatcaa tcctcgagat gaaaaaacca gaattgactg ctacttctgt
     3121 tgaaaaattt ttgattgaaa aatttgattc tgtttctgat ttgatgcaat tgtctgaagg
     3181 tgaagaatca agagcttttt cttttgatgt tggtggtaga ggttatgttt tgagagttaa
     3241 ttcttgtgct gatggttttt ataaagatag atatgtttat agacattttg cttctgctgc
     3301 tttgccaatt ccagaagttt tggatattgg tgaattttct gaatctttga cttattgtat
     3361 ttcaagaaga gctcaaggtg ttactttgca agatttgcca gaaactgaat tgccagctgt
     3421 tttgcaacca gttgctgaag ctatggatgc tattgctgct gctgatttgt ctcaaacttc
     3481 tggttttggt ccatttggtc cacaaggtat tggtcaatat actacttgga gagattttat
     3541 ttgtgctatt gctgatccac atgtttatca ttggcaaact gttatggatg atactgtttc
     3601 tgcttctgtt gctcaagcat tggatgaatt gatgttgtgg gctgaagatt gtccagaagt
     3661 tagacatttg gttcatgctg attttggttc taataatgtt ttgactgata atggtagaat
     3721 tactgctgtt attgattggt ctgaagctat gtttggtgat tctcaatatg aagttgctaa
     3781 tatttttttt tggagaccat ggttggcatg tatggaacaa caaactagat attttgaaag
     3841 aagacatcca gaattggctg gttctccaag attgagagct tatatgttga gaattggttt
     3901 ggatcaattg tatcaatctt tggttgatgg taattttgat gatgctgctt gggctcaagg
     3961 tagatgtgat gctattgtta gatcaggtgc tggtactgtt ggtagaactc aaattgctag
     4021 aagatcagct gctgtttgga ctgatggttg tgttgaagtt ttggctgatt ctggtaatag
     4081 aagaccatct actagaccaa gagctaaaga ataaggatcc gagtgaaatt ctggaaatct
     4141 ggaaatctgg ttttgtattc ttgttattct tctttttgtt attacatata taacttgtta
     4201 cttttttaaa aaaatctttg tttattttat aaatatataa aactaaattt aagaaaaaga
     4261 gaaaaatgtt ttatttgaga gattgaaatt ttacttgaat ttagcttagc ttttataaag
     4321 tattattatg taaaaaaaca aaacaaatat acattaaaaa gttaagacta taaaatagcc
     4381 acccaaggca tttctatatc ttgttgttgt tgttttcatc ttctgtatca gaggaactta
     4441 ttttattatt ttcgtcacgg gtattttctc ttgtttgatg attcatccca ttctttccat
     4501 cataaaatca tatggcgctg agaaccgtta tcgataacta aagcagcaac ttcttctata
     4561 aaaatgggtt agtatgacag tcatttaaat aaggaatttt tcagttggct tggtttcaat
     4621 tcaatgttcg tttttttttt tcttgctgtg tttgtgtttg tgttgtttat agttgtgtgc
     4681 actgatcgtc gaaaaaaaaa attcatagtg agccgggaaa tctgtatagc ccagataaca
     4741 acacaagtcc aaactagaaa ctcgtcaaac accaaaagca atgttgaatc aattgccttg
     4801 cacaagtaca cgtaggaaaa cataaaacat tgcaattttg aatattgagc cttttgtcgt
     4861 aacattgatt gataggatta ctcaccgaat ggttttgaaa ccactgccga cagatcaatc
     4921 aatcaatcaa aaaacgtgaa ctttgaaaaa ggggaagaac agatacattg aagttagcca
     4981 tttccactga tcgtcacaac atatctgata aattactttc aaaattataa gctgatgtgt
     5041 gtgtattatt aatgtgacag taacatccca aacgagaaat attatctcga caacaaaaaa
     5101 gtttgatctg aattgaaaat gaagttttcc caccctaccc atttgtcata ttgaaaccaa
     5161 tcaactgatt aatcaatcaa ttagaattga agctaaacta aaacatacca ccgtccattt
     5221 tgaatgatta tattttttta atattaatat cgagataatg tttctaagaa agaaagaaaa
     5281 ccaggagtga aaattagaaa aggaaaggaa aggaaaaaaa gaaaaatctg aaaatatata
     5341 aaaaaaaatt gtttcgttgg caataaatct tggtgagaac agcgaccgaa agcaaataag
     5401 aacaaaatat gagtgtatta cgttgaacaa ctaattaacg tgtgtgtatg gatctttttt
     5461 tcttttttct ctttaaccga ctataaacaa caaacatttt tgggcagtgc acacactact
     5521 taatatacac agcataaatt acacgattag aaacaaatta gcttattaaa ataacctaat
     5581 caaaccgaat attttatggt attatgagta aactatataa tataaatagc acacacccac
     5641 aacaacaaca aaggaaaact aaaaggtttt ttctttttga aaagatcgtt ttctttatta
     5701 ttctctagtt ttgacgctcg acattttatg atggaatgaa tgggatgaat catcaaacaa
     5761 gagaaaatac ccgtgacgaa aataataaaa taagttcctc tgatacagaa gatgaaaaca
     5821 acaacaacaa gatatagaaa tgccttgggt ggctatttta tagtcttaac tttttaatgt
     5881 atatttgttt tgttttttta cataataata ctttataaaa gctaagctaa attcaagtaa
     5941 aatttcaatc tctcaaataa aacatttttc tctttttctt aaatttagtt ttatatattt
     6001 ataaaataaa caaagatttt tttaaaaaag taacaagtta tatatgtaat aacaaaaaga
     6061 agaataacaa gaatacaaaa ccagatttcc agatttccag aatttagatc catgggtttg
     6121 agagttgtcg ctattgacgg tggtgacgaa aaaggtgaat ttgttaaatc attgggtgct
     6181 gaagcttacg ttgatttcac caaagataaa gatattgttg aagctgtcaa gaaagctact
     6241 gatggtggtc cacacggtgc tatcaatgtc tctgtctcag aaagagccat tggtcaatcc
     6301 actgaatatg tcagaacttt aggtaaagtt gttttggttg gtttgccagc tggtactaaa
     6361 attagtaccc cagtctttga tgctgttatc aagaccattc aaattaaagg ttcttatgtc
     6421 ggtaacagaa aagatactgc tgaagccgtt gatttcttca caagaggttt gatcaaatgc
     6481 ccaatcaaga ttgtcgggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc
     6541 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     6601 tccacacaac atacgagccg ggagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     6661 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6721 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6781 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6841 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6901 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6961 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7021 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7081 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7141 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7201 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7261 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7321 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7381 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7441 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7501 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7561 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7621 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7681 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7741 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7801 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7861 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7921 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7981 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8041 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8101 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8161 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8221 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8281 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8341 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8401 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8461 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8521 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8581 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8641 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8701 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     8761 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     8821 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     8881 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     8941 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     9001 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     9061 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     9121 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     9181 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     9241 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     9301 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     9361 gagctccacc gcggttgaga tggagccgtc aaatatccaa aacactcttc tttcaactaa
     9421 tttgccgtaa actatctcca tcgtacgatt aagataaaaa aaaaacaatt ctttcttaca
     9481 atatttgata gagacccaat gcaaagccag cgagaaagag gagtattggc attgttgggt
     9541 agtagatgaa tgagtaatct tctagccaaa acaaaataaa ccacaagaac agtaggcaaa
     9601 attgagtaat tgatagcgct tgtagtcttt gtatttaaaa aaacctaatt gtccaaaaat
     9661 tgaaaatcat gagttgcaaa tttgttgaaa tcaccaggta aatagatcat ttagctatat
     9721 cgccttatca ttcttcgtac aagaattata cacaaatacc accaccacac cacccagaat
     9781 ttttacacgt gttttctaaa ttattacttt ccgtggccaa tcaaattgaa atttttcttg
     9841 ttcgaaacgg cagtgcctca aacattatag tagcaagtat tgcctaaaga gtttccgtct
     9901 cgtgcccccc ccctatcccc ttttgtaatt tgccaaaata gctcaaaaag gataatcccg
     9961 cacttttttt ttccgagttc tcgtcatgca agcaagctta ttcagaattt tcagaggtgc
    10021 gagtctcacc actaccactg cagctgcatc ttttacagca acagcaacag c
//
