LOCUS       sequence-361636-       11908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHR-R(HRE-minCMV-BFP-Triplex-2X 
            crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Kempton HR, Love KS, Guo LY, Qi LS
  TITLE     Scalable biological signal recording in mammalian cells using Cas12a
            base editors.
  JOURNAL   Nat Chem Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2.
  PUBMED    35637351
REFERENCE   2  (bases 1 to 11908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2."
COMMENT     Sequence Label: pSLQ11434
FEATURES             Location/Qualifiers
     source          1..11908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(679..1008)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      693..828
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     993..1013
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(1083..1100)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1254..1842)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1334..1353)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2013..2804,2805..2873))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2636..2655
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2874..2978)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3046..3064
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3102..3124)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3224..3243
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3457..3476
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3766..3784
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3766..3783
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             3809..4042
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4126..4714
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4232..4243
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4641..4661
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4749..5495)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(5508..5561)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5571..6593)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6604..6815)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     promoter        7012..7049
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7046..7070
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7100..7801
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    8345..8462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8747..8791)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8976..9209
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9706..9831
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9878..10511
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    10679..10813
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(11238..11258)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          11388..11453
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 cagtgccagg cgttgaaaag attagcgacc ggagattggc gggacgaata cgacgcccat
       61 atcccacggc tgttcaatcc aggtatcttg cgggatatca acaacatagt catcaaccag
      121 cggacgacca gccggttttg cgaagatggt gacaaagtgc gcttttggat acatttcacg
      181 aatcgcaacc gcagtaccac cggtatccac caggtcatca ataacgatga agccttcgcc
      241 atcgccttct gcgcgtttca gcactttaag ctcgcgctgg ttgtcgtgat cgtagctgga
      301 aatacaaacg gtatcgacat gacgaatacc cagttcacgc gccagtaacg cacccggtac
      361 agaccgccac ggcttacggc aataatgcct ttccattgtt cagaaggcat cagtcggctt
      421 gcgagtttac gtgcatggat ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg
      481 tgaagtgtcc cagcctgttt atctacggct taaaaagtgt tcgaggggaa aataggttgc
      541 gcgagattat agagatctgg cgcactaaaa accagtattt cacatgagtc cgcgtctttt
      601 tacgcactgc ctctccctga cgcgggataa agtggtattc tcaaacatat ctcgcaagcc
      661 tgtcttgtgt ccaagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
      721 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
      781 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
      841 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      901 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
      961 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg
     1021 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     1081 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1141 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1201 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1261 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1321 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1381 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1441 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1501 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1561 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1621 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1681 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1741 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1801 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1861 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1921 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1981 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     2041 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     2101 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2161 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2221 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2281 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     2341 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2401 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2461 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2521 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2581 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     2641 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2701 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2761 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2821 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2881 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2941 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     3001 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     3061 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     3121 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     3181 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     3241 gtactgagag tgcaccattc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
     3301 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
     3361 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     3421 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     3481 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatctg
     3541 gctagcgatg accctgctga ttggttcgct gaccatttcc gggtgcggga cggcgttacc
     3601 agaaactcag aaggttcgtc caaccaaacc gactctgacg gcagtttacg agagagatga
     3661 tagggtctgc ttcagtaagc cagatgctac acaattaggc ttgtacatat tgtcgttaga
     3721 acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta
     3781 tagaatacac ggaattaatt ctagatgctg ctagagattt tccacactga ctaaaagggt
     3841 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
     3901 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
     3961 gctcagatct ggtctaacca gagagaccca gtacaagcaa aaagcagatc ttgtcttcgt
     4021 tgggagtgaa ttagcccttc cagtcccccc ttttctttta aaaagtggct aagatctaca
     4081 gctgccttgt aagtcattgg tcttaaaggt actcgacggt atcatgcggg gaggcggccc
     4141 aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg
     4201 gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg tagcagaagg
     4261 acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccaaggaaag gacgatgatt
     4321 tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg
     4381 caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg aaagtcccgg
     4441 aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag
     4501 tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga gacagcaacc
     4561 aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca tgatacaaag
     4621 gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga ataccagtca
     4681 atctttcaca aattttgtaa tccagaggtt gattatcgat aagcttgata tcaagcttgc
     4741 gtttaaactt agcctggcag catatcgaga tcgaagtcat cgagagcgtc agcagggagc
     4801 atatccagat caaaatcatc cagggcatcg gcgggcagca tgtccaggtc gaagtcgtcc
     4861 agggcgtcgg tgggtccgcc gctctcgcac ttcagctgct tctccaggcc gcagatgatc
     4921 agttccaggc cgaacaggaa ggcgggctcg gcgccctgtc tgtcgaacag ctcgatggcc
     4981 tgcttcagca gggggggcat gctgtcggtg gtgggtgtct ctctttcctc tttggcgacc
     5041 tggtgttcct gttcttccag cacgcagccc agggtgaagt ggcccacggc gctcagggcg
     5101 tacagggcgt tttccaggct gaagccctgc tggcacagga aggccagctg gttttccagt
     5161 gtctcgtact gcttctcggt gggtctggtg cccaggtgca ctttggcgcc gtcccggtgg
     5221 ctcagcaggg cgcatctgta gctcttggcg ttgttccgca ggaagtcctg ccagctctcg
     5281 ccttccagag ggcagctgtg ggtgtggtgc cggtccagca tctcgatggg cagggcgtcc
     5341 agcagggccc gcttgttctt cacgtgccag tacagggtgg gctgttccac gcccagtttc
     5401 tgggccagct tccgggtggt caggccctcg atgcccacgc cgttcagcag ttccagggcg
     5461 ctgttgatca ctttgctctt gtccagccgg ctcatcgacc ctccgcctgg gccaggattc
     5521 tcctcgacgt caccgcatgt tagcagactt cctctgccct cggaaccgcc ttcctttgcc
     5581 ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg
     5641 gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat
     5701 cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc
     5761 aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat
     5821 cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac
     5881 cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg
     5941 ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa
     6001 tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg
     6061 agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca
     6121 tggggatcag caatcgcgca gatgaaatca cgccatgtag tgtattgacc gattccttgc
     6181 ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg
     6241 gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg
     6301 acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaactc cccaatgtca
     6361 agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg
     6421 tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag
     6481 ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac
     6541 ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt catggtaccg
     6601 gtcctgtgtt ctggcggcaa acccgttgcg aaaaagaacg ttcacggcga ctactgcact
     6661 tatatacggt tctcccccac cctcgggaaa aaggcggagc cagtacacga catcactttc
     6721 ccagtttacc ccgcgccacc ttctctaggc accggatcaa ttgccgaccc ctccccccaa
     6781 cttctcgggg actgtgggcg atgtgcgctc tgcccgtcga catatggtct tcactcgagc
     6841 ggatccgacc ttgagtacgt gcgtctctgc acgtatggcg attaagacct tgagtacgtg
     6901 cgtctctgca cgtatggcga ttaagacctt gagtacgtgc gtctctgcac gtatggcgat
     6961 taagaccttg agtacgtgcg tctctgcacg tatggcgatt aatccatatg cgtaggcgtg
     7021 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc ctagtctact
     7081 acccagaaag cttgaattca tgagcgagct gattaaggag aacatgcaca tgaagctgta
     7141 catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc
     7201 ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc
     7261 cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca
     7321 gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac
     7381 cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg
     7441 cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca
     7501 gaagaaaaca ctcggctggg aggccttcac cgagactctg tatcctgctg acggcggcct
     7561 ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacat
     7621 caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta
     7681 tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca
     7741 cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaatta
     7801 aacgcgtgat tcgtcagtag ggttgtaaag gtttttcttt tcctgagaaa acaacctttt
     7861 gttttctcag gttttgcttt ttggcctttc cctagcttta aaaaaaaaaa agcaaaactc
     7921 accgaggcag ttccatagga tggcaagatc ctggtattgg tctgcgagga tcctttcttt
     7981 ctttgaattt ctactaagtg tagatactcg attcagttaa caagggtaaa tttctactaa
     8041 gtgtagatac tcgattcagt taacaagggt aaatttctac taagtgtaga tcgagttttt
     8101 ttttgtttct cgagttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8161 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8221 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccga ctagtctagt
     8281 taaaaaattc gatatcaagc ttatcgatcc aaactggatc tctgctgtcc ctgtaataaa
     8341 cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg
     8401 ctattatgtc tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa
     8461 aattgtggat gaatactgcc atttggcgat accgtcgaga tccgttcact aatcgaatgg
     8521 atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg tcgggtcccc
     8581 tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca
     8641 ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga
     8701 ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt
     8761 tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta
     8821 aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt
     8881 gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg
     8941 tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc
     9001 tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca
     9061 gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca
     9121 ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg
     9181 ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca
     9241 ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt
     9301 tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg aagatcagcg
     9361 gccggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta tcttgtctaa
     9421 agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt
     9481 attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag
     9541 tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga atcgttctag
     9601 ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa
     9661 ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc
     9721 atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc
     9781 ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg
     9841 tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac
     9901 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     9961 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
    10021 agctcccagg ctcagatctg gtctaaccag agagacccag tacaggcaaa aagcagctgc
    10081 ttatatgcag gatctgaggg ctcgccactc cccagtcccg cccaggccac gcctccctgg
    10141 aaagtcccca gcggaaagtc ccttgtagca agctcgatat cagcagttct tgaagtactc
    10201 cggatgcagc tctcgggcca cgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
    10261 cacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agctggtgtt
    10321 ctctccttta ttggcctctt ctaccttatc tggctcaact ggtactagct tgtagcacca
    10381 tccaaaggtc agtggatatc tgacccctgg ccctggtgtg tagttctgct aatcagggaa
    10441 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcttt gggagtgaat
    10501 tagcccttcc aactactaag tttgtagtac atatttaaca aatacaattt ctttaaaatg
    10561 aaaataattc agaggaatca caggtttaga gtaaatgaaa ccacaggtaa ttggcagtgg
    10621 taatagggta tggggtggga agtttgggat gattttggtt agcttgagtt atccagttga
    10681 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
    10741 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
    10801 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
    10861 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct
    10921 ctagtcaagg cactatacat caaatattcc ttattaaccc ctttacaaat taaaaagcta
    10981 aaggtacaca atttttgagc atagttatta atagcagaca ctctatgcct gtgtggagta
    11041 agaaaaaaca gtatgttatg attataactg ttatgcctac ttataaaggt tacagaatat
    11101 ttttccataa ttttcttgta tagcagtgca gctttttcct ttgtggtgta aatagcaaag
    11161 caagcaagag ttctattact aaacacagca tgactcaaaa aacttagcaa ttctgaagga
    11221 aagtccttgg ggtcttctac ctttctcttc ttttttggag gagtagaatg ttgagagtca
    11281 gcagtagcct catcatcact agatggcatt tcttctgagc aaaacaggtt ttcctcatta
    11341 aaggcattcc accactgctc ccattcatca gttccatagg ttggaatcta aaatacacaa
    11401 acaattagaa tcagtagttt aacacattat acacttaaaa attttatatt taccttagag
    11461 ctttaaatct ctgtaggtag tttgtccaat tatgtcacac cacagaagta aggttccttc
    11521 acaaagatcc ggggcccact cataaatcca gttgccgcca cggtagccaa tcaccgtatc
    11581 gtataaatca tcgtcggtac gttcggcatc gctcatcaca atacgtgcct ggacgtcgag
    11641 gatttcgcgt gggtcaatgc cgcgccagat ccacatcaga cggttaatca tgcgatacca
    11701 gtgagggatg gttttaccat caagggccga ctgcacaggc ggttgtgcgc cgtgattaaa
    11761 gcggcggact agcgtcgagg tttcaggatg tttaaagcgg ggtttgaaca gggtttcgct
    11821 caggtttgcc tgtgtcatgg atgcagcctc cagaatactt actggaaact attgtaaccc
    11881 gcctgaagtt aaaaagaaca acgcccgg
//
