LOCUS       sequence-361637-       11818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHR-R(ARE-minCMV-BFP-Triplex-2X 
            crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11818)
  AUTHORS   Kempton HR, Love KS, Guo LY, Qi LS
  TITLE     Scalable biological signal recording in mammalian cells using Cas12a
            base editors.
  JOURNAL   Nat Chem Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2.
  PUBMED    35637351
REFERENCE   2  (bases 1 to 11818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2."
COMMENT     Sequence Label: pSLQ11445
FEATURES             Location/Qualifiers
     source          1..11818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          468..533
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             663..683
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1108..1242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             1410..2043
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2090..2215
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2712..2945
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3130..3174
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3459..3576
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4120..4821)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4851..4875)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(4872..4909)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        5016..5227
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5238..6260
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6270..6323
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6336..7082
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7117..7705
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7170..7190)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7588..7599)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7789..8022
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8047..8065)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8048..8065)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8355..8374)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8588..8607)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8707..8729
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8767..8785)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8853..8957
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8958..9026,9027..9818)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9176..9195)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9989..10577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10478..10497
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10731..10748
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10818..10838)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10823..11152
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11003..11138
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt
       61 aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc
      121 ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc
      181 gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg
      241 tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat
      301 tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg
      361 tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg
      421 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
      481 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
      541 cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt
      601 gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc
      661 ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt
      721 ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa
      781 aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa
      841 gtaggcataa cagttataat cataacatac tgttttttct tactccacac aggcatagag
      901 tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag
      961 gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc
     1021 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     1081 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     1141 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     1201 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
     1261 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
     1321 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
     1381 tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca
     1441 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     1501 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     1561 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     1621 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     1681 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     1741 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     1801 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     1861 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     1921 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     1981 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     2041 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     2101 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     2161 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     2221 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     2281 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     2341 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     2401 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     2461 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     2521 gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga
     2581 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     2641 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     2701 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     2761 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     2821 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     2881 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     2941 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     3001 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     3061 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     3121 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     3181 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     3241 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     3301 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     3361 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     3421 tctcgacggt atcgccaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     3481 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     3541 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     3601 gatccagttt ggatcgataa gcttgatatc gaatttttta actagactag tcggttagct
     3661 tgagttatcc agttgatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     3721 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     3781 taaccattat aagctgcaat aaacaactcg agaaacaaaa aaaaactcga tctacactta
     3841 gtagaaattt acccttgtta actgaatcga gtatctacac ttagtagaaa tttacccttg
     3901 ttaactgaat cgagtatcta cacttagtag aaattcaaag aaagaaagga tcctcgcaga
     3961 ccaataccag gatcttgcca tcctatggaa ctgcctcggt gagttttgct tttttttttt
     4021 taaagctagg gaaaggccaa aaagcaaaac ctgagaaaac aaaaggttgt tttctcagga
     4081 aaagaaaaac ctttacaacc ctactgacga atcacgcgtt taattaagct tgtgccccag
     4141 tttgctaggg aggtcgcagt atctggccac tgccacctcg tgctgctcga cgtaggtctc
     4201 gttgttggcc tccttgattc tttccagtct gtagtccaca tagtagacgc caggcatctt
     4261 gaggttctta gcgggtttct tggatctata tgtggtcttg atgtttgcga tcagatggct
     4321 cccgcccacg agcttcaggg ccatgtcgtt tctgccttcc aggccgccgt cagcaggata
     4381 cagagtctcg gtgaaggcct cccagccgag tgttttcttc tgcatcacag ggccgttgga
     4441 tgtgaagttc acccctctga tcttgacgtt gtagatgagg cagccgtcct ggaggctggt
     4501 gtcctgggta gcggtcagca cgcccccgtc ttcgtatgtg gtgactctct cccatgtgaa
     4561 gccctcaggg aaggactgct tgaagaagtc ggggatgccc tgggtgtggt tgatgaaggt
     4621 cttgctgccg tagaggaagc tagtagccag gatgtcgaag gcgaagggga gagggccgcc
     4681 ctcgaccacc ttgattctca tggtctgggt gccctcgtag ggcttgcctt cgccctcgga
     4741 tgtgcacttg aagtgatggt tgtccacggt gccctccatg tacagcttca tgtgcatgtt
     4801 ctccttaatc agctcgctca tgaattcaag ctttctgggt agtagactag gatctgacgg
     4861 ttcactaaac gagctctgct tatatagacc tcccaccgta cacgcctacg catatgtcga
     4921 gcccgggaaa gttgctttgt caccattagc aatgtcattt ccaagctaaa agttgctttg
     4981 tcaccattag caatgtcatt tccaagctag tcgacgggca gagcgcacat cgcccacagt
     5041 ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg
     5101 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     5161 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     5221 aacacaggac cggtaccatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     5281 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     5341 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     5401 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     5461 cggaagtgct tgacattggg gagttcagcg agagcctgac ctattgcatc tcccgccgtg
     5521 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     5581 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     5641 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcatc tgcgcgattg
     5701 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     5761 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     5821 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     5881 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     5941 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     6001 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     6061 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     6121 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     6181 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     6241 ctcgtccgag ggcaaaggaa ggcggttccg agggcagagg aagtctgcta acatgcggtg
     6301 acgtcgagga gaatcctggc ccaggcggag ggtcgatgag ccggctggac aagagcaaag
     6361 tgatcaacag cgccctggaa ctgctgaacg gcgtgggcat cgagggcctg accacccgga
     6421 agctggccca gaaactgggc gtggaacagc ccaccctgta ctggcacgtg aagaacaagc
     6481 gggccctgct ggacgccctg cccatcgaga tgctggaccg gcaccacacc cacagctgcc
     6541 ctctggaagg cgagagctgg caggacttcc tgcggaacaa cgccaagagc tacagatgcg
     6601 ccctgctgag ccaccgggac ggcgccaaag tgcacctggg caccagaccc accgagaagc
     6661 agtacgagac actggaaaac cagctggcct tcctgtgcca gcagggcttc agcctggaaa
     6721 acgccctgta cgccctgagc gccgtgggcc acttcaccct gggctgcgtg ctggaagaac
     6781 aggaacacca ggtcgccaaa gaggaaagag agacacccac caccgacagc atgccccccc
     6841 tgctgaagca ggccatcgag ctgttcgaca gacagggcgc cgagcccgcc ttcctgttcg
     6901 gcctggaact gatcatctgc ggcctggaga agcagctgaa gtgcgagagc ggcggaccca
     6961 ccgacgccct ggacgacttc gacctggaca tgctgcccgc cgatgccctg gatgattttg
     7021 atctggatat gctccctgct gacgctctcg atgacttcga tctcgatatg ctgccaggct
     7081 aagtttaaac gcaagcttga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     7141 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7201 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7261 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7321 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7381 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7441 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7501 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     7561 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7621 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7681 atctcccttt gggccgcctc cccgcatgat accgtcgagt acctttaaga ccaatgactt
     7741 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7801 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7861 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7921 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7981 gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc
     8041 gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt
     8101 gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa
     8161 gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt
     8221 ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt
     8281 catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
     8341 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
     8401 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
     8461 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     8521 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca
     8581 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     8641 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     8701 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     8761 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     8821 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8881 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8941 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     9001 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9061 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9121 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     9181 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     9241 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9301 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9361 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9421 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9481 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9541 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9601 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9661 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9721 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9781 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9841 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9901 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9961 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    10021 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10081 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    10141 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    10201 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    10261 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10321 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10381 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10441 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10501 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10561 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    10621 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    10681 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    10741 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    10801 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
    10861 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
    10921 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
    10981 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    11041 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    11101 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
    11161 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
    11221 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
    11281 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
    11341 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
    11401 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
    11461 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
    11521 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
    11581 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
    11641 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
    11701 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
    11761 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaa
//
