LOCUS       sequence-361638-       11784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHR-R(AP1-minCMV-BFP-Triplex-2X 
            crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Kempton HR, Love KS, Guo LY, Qi LS
  TITLE     Scalable biological signal recording in mammalian cells using Cas12a
            base editors.
  JOURNAL   Nat Chem Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2.
  PUBMED    35637351
REFERENCE   2  (bases 1 to 11784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2."
COMMENT     Sequence Label: pSLQ11444
FEATURES             Location/Qualifiers
     source          1..11784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(643..972)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      657..792
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     957..977
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(1047..1064)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1218..1806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1298..1317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1977..2768,2769..2837))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2600..2619
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2838..2942)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3010..3028
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3066..3088)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3188..3207
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3421..3440
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3730..3748
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3730..3747
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             3773..4006
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4090..4678
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4196..4207
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4605..4625
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4713..5459)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(5472..5525)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5535..6557)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6568..6779)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     promoter        6852..6889
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6886..6910
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6940..7641
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    8185..8302
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8587..8631)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8816..9049
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9546..9671
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9718..10351
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    10519..10653
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(11078..11098)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          11228..11293
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 tggcgggacg aatacgacgc ccatatccca cggctgttca atccaggtat cttgcgggat
       61 atcaacaaca tagtcatcaa ccagcggacg accagccggt tttgcgaaga tggtgacaaa
      121 gtgcgctttt ggatacattt cacgaatcgc aaccgcagta ccaccggtat ccaccaggtc
      181 atcaataacg atgaagcctt cgccatcgcc ttctgcgcgt ttcagcactt taagctcgcg
      241 ctggttgtcg tgatcgtagc tggaaataca aacggtatcg acatgacgaa tacccagttc
      301 acgcgccagt aacgcacccg gtacagaccg ccacggctta cggcaataat gcctttccat
      361 tgttcagaag gcatcagtcg gcttgcgagt ttacgtgcat ggatctgcaa catgtcccag
      421 gtgacgatgt atttttcgct catgtgaagt gtcccagcct gtttatctac ggcttaaaaa
      481 gtgttcgagg ggaaaatagg ttgcgcgaga ttatagagat ctggcgcact aaaaaccagt
      541 atttcacatg agtccgcgtc tttttacgca ctgcctctcc ctgacgcggg ataaagtggt
      601 attctcaaac atatctcgca agcctgtctt gtgtccaagc tttttgcaaa agcctaggcc
      661 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
      721 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
      781 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
      841 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
      901 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
      961 taactgacac acattccaca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1021 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1081 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1141 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1201 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1261 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1321 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1381 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1441 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1501 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1561 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1621 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1681 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1741 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1801 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1861 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1921 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1981 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2041 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2101 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2161 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2221 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2281 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2341 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2401 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2461 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2521 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2581 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2641 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2701 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2761 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2821 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     2881 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2941 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     3001 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     3061 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     3121 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     3181 aactatgcgg catcagagca gattgtactg agagtgcacc attcgacgct ctcccttatg
     3241 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
     3301 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     3361 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     3421 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     3481 gatgcgtccg gcgtagagga tctggctagc gatgaccctg ctgattggtt cgctgaccat
     3541 ttccgggtgc gggacggcgt taccagaaac tcagaaggtt cgtccaacca aaccgactct
     3601 gacggcagtt tacgagagag atgatagggt ctgcttcagt aagccagatg ctacacaatt
     3661 aggcttgtac atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat
     3721 acacatacga tttaggtgac actatagaat acacggaatt aattctagat gctgctagag
     3781 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     3841 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     3901 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacaa
     3961 gcaaaaagca gatcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct
     4021 tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa aggtactcga
     4081 cggtatcatg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa
     4141 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga
     4201 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga
     4261 gcagccaagg aaaggacgat gatttccccg acaacaccac ggaattgtca gtgcccaaca
     4321 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag
     4381 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag
     4441 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca
     4501 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac
     4561 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag
     4621 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattat
     4681 cgataagctt gatatcaagc ttgcgtttaa acttagcctg gcagcatatc gagatcgaag
     4741 tcatcgagag cgtcagcagg gagcatatcc agatcaaaat catccagggc atcggcgggc
     4801 agcatgtcca ggtcgaagtc gtccagggcg tcggtgggtc cgccgctctc gcacttcagc
     4861 tgcttctcca ggccgcagat gatcagttcc aggccgaaca ggaaggcggg ctcggcgccc
     4921 tgtctgtcga acagctcgat ggcctgcttc agcagggggg gcatgctgtc ggtggtgggt
     4981 gtctctcttt cctctttggc gacctggtgt tcctgttctt ccagcacgca gcccagggtg
     5041 aagtggccca cggcgctcag ggcgtacagg gcgttttcca ggctgaagcc ctgctggcac
     5101 aggaaggcca gctggttttc cagtgtctcg tactgcttct cggtgggtct ggtgcccagg
     5161 tgcactttgg cgccgtcccg gtggctcagc agggcgcatc tgtagctctt ggcgttgttc
     5221 cgcaggaagt cctgccagct ctcgccttcc agagggcagc tgtgggtgtg gtgccggtcc
     5281 agcatctcga tgggcagggc gtccagcagg gcccgcttgt tcttcacgtg ccagtacagg
     5341 gtgggctgtt ccacgcccag tttctgggcc agcttccggg tggtcaggcc ctcgatgccc
     5401 acgccgttca gcagttccag ggcgctgttg atcactttgc tcttgtccag ccggctcatc
     5461 gaccctccgc ctgggccagg attctcctcg acgtcaccgc atgttagcag acttcctctg
     5521 ccctcggaac cgccttcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg
     5581 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
     5641 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
     5701 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
     5761 atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
     5821 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat
     5881 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc
     5941 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc
     6001 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc
     6061 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcagatgaa atcacgccat
     6121 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga
     6181 tcggccgcag cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg
     6241 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg
     6301 ctctcgctga actccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag
     6361 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca
     6421 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc
     6481 atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg
     6541 agttcaggct ttttcatggt accggtcctg tgttctggcg gcaaacccgt tgcgaaaaag
     6601 aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg
     6661 gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct aggcaccgga
     6721 tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc gctctgcccg
     6781 tcgactgagt cagtgactca gtgagtcagt gactcagtga gtcagtgact cagcccgggc
     6841 tcgacatatg cgtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     6901 ccgtcagatc ctagtctact acccagaaag cttgaattca tgagcgagct gattaaggag
     6961 aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca
     7021 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag
     7081 ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag
     7141 accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc
     7201 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
     7261 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca
     7321 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagactctg
     7381 tatcctgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg
     7441 agccatctga tcgcaaacat caagaccaca tatagatcca agaaacccgc taagaacctc
     7501 aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac
     7561 gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa
     7621 ctggggcaca agcttaatta aacgcgtgat tcgtcagtag ggttgtaaag gtttttcttt
     7681 tcctgagaaa acaacctttt gttttctcag gttttgcttt ttggcctttc cctagcttta
     7741 aaaaaaaaaa agcaaaactc accgaggcag ttccatagga tggcaagatc ctggtattgg
     7801 tctgcgagga tcctttcttt ctttgaattt ctactaagtg tagatactcg attcagttaa
     7861 caagggtaaa tttctactaa gtgtagatac tcgattcagt taacaagggt aaatttctac
     7921 taagtgtaga tcgagttttt ttttgtttct cgagttgttt attgcagctt ataatggtta
     7981 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8041 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     8101 agctaaccga ctagtctagt taaaaaattc gatatcaagc ttatcgatcc aaactggatc
     8161 tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc
     8221 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc
     8281 aatcccccct tttcttttaa aattgtggat gaatactgcc atttggcgat accgtcgaga
     8341 tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc
     8401 ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata
     8461 tccctgccta actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa
     8521 gcctcctact atcattatga ataattttat ataccacagc caatttgtta tgttaaacca
     8581 attccacaaa cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg
     8641 gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc
     8701 ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc
     8761 attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag
     8821 ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc
     8881 ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg
     8941 ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc
     9001 gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc
     9061 tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc
     9121 aatttttact actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc
     9181 tccaggtctg aagatcagcg gccggccgct tgctgtgcgg tggtcttact tttgttttgc
     9241 tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca
     9301 caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg
     9361 gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga
     9421 ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt
     9481 ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta
     9541 atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc
     9601 gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg
     9661 cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc
     9721 tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca
     9781 acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg
     9841 ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag
     9901 tacaggcaaa aagcagctgc ttatatgcag gatctgaggg ctcgccactc cccagtcccg
     9961 cccaggccac gcctccctgg aaagtcccca gcggaaagtc ccttgtagca agctcgatat
    10021 cagcagttct tgaagtactc cggatgcagc tctcgggcca cgtgatgaaa tgctaggcgg
    10081 ctgtcaaacc tccactctaa cacttctctc tccgggtcat ccatcccatg caggctcaca
    10141 gggtgtaaca agctggtgtt ctctccttta ttggcctctt ctaccttatc tggctcaact
    10201 ggtactagct tgtagcacca tccaaaggtc agtggatatc tgacccctgg ccctggtgtg
    10261 tagttctgct aatcagggaa gtagccttgt gtgtggtaga tccacagatc aaggatatct
    10321 tgtcttcttt gggagtgaat tagcccttcc aactactaag tttgtagtac atatttaaca
    10381 aatacaattt ctttaaaatg aaaataattc agaggaatca caggtttaga gtaaatgaaa
    10441 ccacaggtaa ttggcagtgg taatagggta tggggtggga agtttgggat gattttggtt
    10501 agcttgagtt atccagttga tccagacatg ataagataca ttgatgagtt tggacaaacc
    10561 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
    10621 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
    10681 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
    10741 ggtatggctg attatgatct ctagtcaagg cactatacat caaatattcc ttattaaccc
    10801 ctttacaaat taaaaagcta aaggtacaca atttttgagc atagttatta atagcagaca
    10861 ctctatgcct gtgtggagta agaaaaaaca gtatgttatg attataactg ttatgcctac
    10921 ttataaaggt tacagaatat ttttccataa ttttcttgta tagcagtgca gctttttcct
    10981 ttgtggtgta aatagcaaag caagcaagag ttctattact aaacacagca tgactcaaaa
    11041 aacttagcaa ttctgaagga aagtccttgg ggtcttctac ctttctcttc ttttttggag
    11101 gagtagaatg ttgagagtca gcagtagcct catcatcact agatggcatt tcttctgagc
    11161 aaaacaggtt ttcctcatta aaggcattcc accactgctc ccattcatca gttccatagg
    11221 ttggaatcta aaatacacaa acaattagaa tcagtagttt aacacattat acacttaaaa
    11281 attttatatt taccttagag ctttaaatct ctgtaggtag tttgtccaat tatgtcacac
    11341 cacagaagta aggttccttc acaaagatcc ggggcccact cataaatcca gttgccgcca
    11401 cggtagccaa tcaccgtatc gtataaatca tcgtcggtac gttcggcatc gctcatcaca
    11461 atacgtgcct ggacgtcgag gatttcgcgt gggtcaatgc cgcgccagat ccacatcaga
    11521 cggttaatca tgcgatacca gtgagggatg gttttaccat caagggccga ctgcacaggc
    11581 ggttgtgcgc cgtgattaaa gcggcggact agcgtcgagg tttcaggatg tttaaagcgg
    11641 ggtttgaaca gggtttcgct caggtttgcc tgtgtcatgg atgcagcctc cagaatactt
    11701 actggaaact attgtaaccc gcctgaagtt aaaaagaaca acgcccggca gtgccaggcg
    11761 ttgaaaagat tagcgaccgg agat
//
