LOCUS       sequence-361640-       11863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHR-R(NFAT-minCMV-BFP-Triplex-2X 
            crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11863)
  AUTHORS   Kempton HR, Love KS, Guo LY, Qi LS
  TITLE     Scalable biological signal recording in mammalian cells using Cas12a
            base editors.
  JOURNAL   Nat Chem Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2.
  PUBMED    35637351
REFERENCE   2  (bases 1 to 11863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2."
COMMENT     Sequence Label: pSLQ11443
FEATURES             Location/Qualifiers
     source          1..11863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(614..943)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      628..763
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     928..948
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(1018..1035)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1189..1777)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1269..1288)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1948..2739,2740..2808))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2571..2590
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2809..2913)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2981..2999
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3037..3059)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3159..3178
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3392..3411
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3701..3719
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3701..3718
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             3744..3977
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4061..4649
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4167..4178
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4576..4596
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4684..5430)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(5443..5496)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5506..6528)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6539..6750)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    6782..6811
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    6812..6841
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    6842..6871
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
     promoter        6902..6939
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6936..6960
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6990..7691
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    8235..8352
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8637..8681)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8866..9099
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9596..9721
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9768..10401
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    10569..10703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(11128..11148)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          11278..11343
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 acggctgttc aatccaggta tcttgcggga tatcaacaac atagtcatca accagcggac
       61 gaccagccgg ttttgcgaag atggtgacaa agtgcgcttt tggatacatt tcacgaatcg
      121 caaccgcagt accaccggta tccaccaggt catcaataac gatgaagcct tcgccatcgc
      181 cttctgcgcg tttcagcact ttaagctcgc gctggttgtc gtgatcgtag ctggaaatac
      241 aaacggtatc gacatgacga atacccagtt cacgcgccag taacgcaccc ggtacagacc
      301 gccacggctt acggcaataa tgcctttcca ttgttcagaa ggcatcagtc ggcttgcgag
      361 tttacgtgca tggatctgca acatgtccca ggtgacgatg tatttttcgc tcatgtgaag
      421 tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag gggaaaatag gttgcgcgag
      481 attatagaga tctggcgcac taaaaaccag tatttcacat gagtccgcgt ctttttacgc
      541 actgcctctc cctgacgcgg gataaagtgg tattctcaaa catatctcgc aagcctgtct
      601 tgtgtccaag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
      661 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
      721 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
      781 ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
      841 ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc
      901 ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac agctgcatta
      961 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     1021 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1081 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1141 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1201 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1261 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1321 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1381 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1441 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1501 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1561 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1621 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1681 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1741 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1801 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1861 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1921 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     1981 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2041 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2101 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2161 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2221 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2281 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2341 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2401 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2461 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2521 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     2581 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2641 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2701 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     2761 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     2821 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     2881 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     2941 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     3001 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     3061 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     3121 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     3181 gagagtgcac cattcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     3241 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     3301 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     3361 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     3421 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atctggctag
     3481 cgatgaccct gctgattggt tcgctgacca tttccgggtg cgggacggcg ttaccagaaa
     3541 ctcagaaggt tcgtccaacc aaaccgactc tgacggcagt ttacgagaga gatgataggg
     3601 tctgcttcag taagccagat gctacacaat taggcttgta catattgtcg ttagaacgcg
     3661 gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa
     3721 tacacggaat taattctaga tgctgctaga gattttccac actgactaaa agggtctgag
     3781 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     3841 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     3901 gatctggtct aaccagagag acccagtaca agcaaaaagc agatcttgtc ttcgttggga
     3961 gtgaattagc ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc
     4021 cttgtaagtc attggtctta aaggtactcg acggtatcat gcggggaggc ggcccaaagg
     4081 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
     4141 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
     4201 ccgcgcagaa tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc
     4261 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
     4321 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
     4381 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
     4441 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     4501 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     4561 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     4621 tcacaaattt tgtaatccag aggttgatta tcgataagct tgatatcaag cttgcgttta
     4681 aacttagcct ggcagcatat cgagatcgaa gtcatcgaga gcgtcagcag ggagcatatc
     4741 cagatcaaaa tcatccaggg catcggcggg cagcatgtcc aggtcgaagt cgtccagggc
     4801 gtcggtgggt ccgccgctct cgcacttcag ctgcttctcc aggccgcaga tgatcagttc
     4861 caggccgaac aggaaggcgg gctcggcgcc ctgtctgtcg aacagctcga tggcctgctt
     4921 cagcaggggg ggcatgctgt cggtggtggg tgtctctctt tcctctttgg cgacctggtg
     4981 ttcctgttct tccagcacgc agcccagggt gaagtggccc acggcgctca gggcgtacag
     5041 ggcgttttcc aggctgaagc cctgctggca caggaaggcc agctggtttt ccagtgtctc
     5101 gtactgcttc tcggtgggtc tggtgcccag gtgcactttg gcgccgtccc ggtggctcag
     5161 cagggcgcat ctgtagctct tggcgttgtt ccgcaggaag tcctgccagc tctcgccttc
     5221 cagagggcag ctgtgggtgt ggtgccggtc cagcatctcg atgggcaggg cgtccagcag
     5281 ggcccgcttg ttcttcacgt gccagtacag ggtgggctgt tccacgccca gtttctgggc
     5341 cagcttccgg gtggtcaggc cctcgatgcc cacgccgttc agcagttcca gggcgctgtt
     5401 gatcactttg ctcttgtcca gccggctcat cgaccctccg cctgggccag gattctcctc
     5461 gacgtcaccg catgttagca gacttcctct gccctcggaa ccgccttcct ttgccctcgg
     5521 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
     5581 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
     5641 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
     5701 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccgcg gcgatcctgc
     5761 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
     5821 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
     5881 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
     5941 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
     6001 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
     6061 atcagcaatc gcgcagatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
     6121 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc
     6181 cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
     6241 ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aactccccaa tgtcaagcac
     6301 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
     6361 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
     6421 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
     6481 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatgg taccggtcct
     6541 gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact gcacttatat
     6601 acggttctcc cccaccctcg ggaaaaaggc ggagccagta cacgacatca ctttcccagt
     6661 ttaccccgcg ccaccttctc taggcaccgg atcaattgcc gacccctccc cccaacttct
     6721 cggggactgt gggcgatgtg cgctctgccc gtcgacatat ggattacctg agctcgctag
     6781 cggaggaaaa actgtttcat acagaaggcg tggaggaaaa actgtttcat acagaaggcg
     6841 tggaggaaaa actgtttcat acagaaggcg tagatctaga ctccccgggc tcgacatatg
     6901 cgtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
     6961 ctagtctact acccagaaag cttgaattca tgagcgagct gattaaggag aacatgcaca
     7021 tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg
     7081 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc
     7141 tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca
     7201 accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg
     7261 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
     7321 aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc
     7381 ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagactctg tatcctgctg
     7441 acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga
     7501 tcgcaaacat caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg
     7561 gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg
     7621 tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca
     7681 agcttaatta aacgcgtgat tcgtcagtag ggttgtaaag gtttttcttt tcctgagaaa
     7741 acaacctttt gttttctcag gttttgcttt ttggcctttc cctagcttta aaaaaaaaaa
     7801 agcaaaactc accgaggcag ttccatagga tggcaagatc ctggtattgg tctgcgagga
     7861 tcctttcttt ctttgaattt ctactaagtg tagatactcg attcagttaa caagggtaaa
     7921 tttctactaa gtgtagatac tcgattcagt taacaagggt aaatttctac taagtgtaga
     7981 tcgagttttt ttttgtttct cgagttgttt attgcagctt ataatggtta caaataaagc
     8041 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8101 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccga
     8161 ctagtctagt taaaaaattc gatatcaagc ttatcgatcc aaactggatc tctgctgtcc
     8221 ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc tttagtttgt
     8281 atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc aatcccccct
     8341 tttcttttaa aattgtggat gaatactgcc atttggcgat accgtcgaga tccgttcact
     8401 aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg
     8461 tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
     8521 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
     8581 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
     8641 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
     8701 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
     8761 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
     8821 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
     8881 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
     8941 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
     9001 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
     9061 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
     9121 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
     9181 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg
     9241 aagatcagcg gccggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta
     9301 tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg
     9361 ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta
     9421 gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga
     9481 atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc
     9541 ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc
     9601 tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca
     9661 gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga
     9721 gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt
     9781 ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc
     9841 acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag
     9901 ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag tacaggcaaa
     9961 aagcagctgc ttatatgcag gatctgaggg ctcgccactc cccagtcccg cccaggccac
    10021 gcctccctgg aaagtcccca gcggaaagtc ccttgtagca agctcgatat cagcagttct
    10081 tgaagtactc cggatgcagc tctcgggcca cgtgatgaaa tgctaggcgg ctgtcaaacc
    10141 tccactctaa cacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca
    10201 agctggtgtt ctctccttta ttggcctctt ctaccttatc tggctcaact ggtactagct
    10261 tgtagcacca tccaaaggtc agtggatatc tgacccctgg ccctggtgtg tagttctgct
    10321 aatcagggaa gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcttt
    10381 gggagtgaat tagcccttcc aactactaag tttgtagtac atatttaaca aatacaattt
    10441 ctttaaaatg aaaataattc agaggaatca caggtttaga gtaaatgaaa ccacaggtaa
    10501 ttggcagtgg taatagggta tggggtggga agtttgggat gattttggtt agcttgagtt
    10561 atccagttga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
    10621 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
    10681 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
    10741 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
    10801 attatgatct ctagtcaagg cactatacat caaatattcc ttattaaccc ctttacaaat
    10861 taaaaagcta aaggtacaca atttttgagc atagttatta atagcagaca ctctatgcct
    10921 gtgtggagta agaaaaaaca gtatgttatg attataactg ttatgcctac ttataaaggt
    10981 tacagaatat ttttccataa ttttcttgta tagcagtgca gctttttcct ttgtggtgta
    11041 aatagcaaag caagcaagag ttctattact aaacacagca tgactcaaaa aacttagcaa
    11101 ttctgaagga aagtccttgg ggtcttctac ctttctcttc ttttttggag gagtagaatg
    11161 ttgagagtca gcagtagcct catcatcact agatggcatt tcttctgagc aaaacaggtt
    11221 ttcctcatta aaggcattcc accactgctc ccattcatca gttccatagg ttggaatcta
    11281 aaatacacaa acaattagaa tcagtagttt aacacattat acacttaaaa attttatatt
    11341 taccttagag ctttaaatct ctgtaggtag tttgtccaat tatgtcacac cacagaagta
    11401 aggttccttc acaaagatcc ggggcccact cataaatcca gttgccgcca cggtagccaa
    11461 tcaccgtatc gtataaatca tcgtcggtac gttcggcatc gctcatcaca atacgtgcct
    11521 ggacgtcgag gatttcgcgt gggtcaatgc cgcgccagat ccacatcaga cggttaatca
    11581 tgcgatacca gtgagggatg gttttaccat caagggccga ctgcacaggc ggttgtgcgc
    11641 cgtgattaaa gcggcggact agcgtcgagg tttcaggatg tttaaagcgg ggtttgaaca
    11701 gggtttcgct caggtttgcc tgtgtcatgg atgcagcctc cagaatactt actggaaact
    11761 attgtaaccc gcctgaagtt aaaaagaaca acgcccggca gtgccaggcg ttgaaaagat
    11821 tagcgaccgg agattggcgg gacgaatacg acgcccatat ccc
//
