LOCUS       sequence-361641-       11891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHR-R(CRE-minCMV-BFP-Triplex-2X 
            crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11891)
  AUTHORS   Kempton HR, Love KS, Guo LY, Qi LS
  TITLE     Scalable biological signal recording in mammalian cells using Cas12a
            base editors.
  JOURNAL   Nat Chem Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2.
  PUBMED    35637351
REFERENCE   2  (bases 1 to 11891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2."
COMMENT     Sequence Label: pSLQ11435
FEATURES             Location/Qualifiers
     source          1..11891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(627..956)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      641..776
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     941..961
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(1031..1048)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1202..1790)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1282..1301)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1961..2752,2753..2821))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2584..2603
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2822..2926)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2994..3012
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3050..3072)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3172..3191
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3405..3424
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3714..3732
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3714..3731
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             3757..3990
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4074..4662
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4180..4191
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4589..4609
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4697..5443)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(5456..5509)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5519..6541)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6552..6763)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     6933..6957
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7031..7732
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    8276..8393
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8678..8722)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8907..9140
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9637..9762
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9809..10442
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    10610..10744
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(11169..11189)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          11319..11384
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 acgcccatat cccacggctg ttcaatccag gtatcttgcg ggatatcaac aacatagtca
       61 tcaaccagcg gacgaccagc cggttttgcg aagatggtga caaagtgcgc ttttggatac
      121 atttcacgaa tcgcaaccgc agtaccaccg gtatccacca ggtcatcaat aacgatgaag
      181 ccttcgccat cgccttctgc gcgtttcagc actttaagct cgcgctggtt gtcgtgatcg
      241 tagctggaaa tacaaacggt atcgacatga cgaataccca gttcacgcgc cagtaacgca
      301 cccggtacag accgccacgg cttacggcaa taatgccttt ccattgttca gaaggcatca
      361 gtcggcttgc gagtttacgt gcatggatct gcaacatgtc ccaggtgacg atgtattttt
      421 cgctcatgtg aagtgtccca gcctgtttat ctacggctta aaaagtgttc gaggggaaaa
      481 taggttgcgc gagattatag agatctggcg cactaaaaac cagtatttca catgagtccg
      541 cgtcttttta cgcactgcct ctccctgacg cgggataaag tggtattctc aaacatatct
      601 cgcaagcctg tcttgtgtcc aagctttttg caaaagccta ggcctccaaa aaagcctcct
      661 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
      721 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
      781 ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg
      841 cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt
      901 tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc
      961 cacagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1021 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1081 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1141 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1201 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1261 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1321 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1381 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1441 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1501 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1561 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1621 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1681 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1741 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1801 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1861 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1921 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1981 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2041 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2101 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2161 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2221 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2281 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2341 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2401 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2461 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2521 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2581 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2641 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2701 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2761 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2821 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     2881 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2941 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     3001 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     3061 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     3121 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     3181 agcagattgt actgagagtg caccattcga cgctctccct tatgcgactc ctgcattagg
     3241 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
     3301 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
     3361 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
     3421 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
     3481 aggatctggc tagcgatgac cctgctgatt ggttcgctga ccatttccgg gtgcgggacg
     3541 gcgttaccag aaactcagaa ggttcgtcca accaaaccga ctctgacggc agtttacgag
     3601 agagatgata gggtctgctt cagtaagcca gatgctacac aattaggctt gtacatattg
     3661 tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg
     3721 tgacactata gaatacacgg aattaattct agatgctgct agagattttc cacactgact
     3781 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
     3841 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
     3901 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaagcaaaa agcagatctt
     3961 gtcttcgttg ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa
     4021 gatctacagc tgccttgtaa gtcattggtc ttaaaggtac tcgacggtat catgcgggga
     4081 ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg
     4141 cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta
     4201 gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc aaggaaagga
     4261 cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca
     4321 gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa
     4381 agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc
     4441 aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga
     4501 cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg
     4561 atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat
     4621 accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttatcgataa gcttgatatc
     4681 aagcttgcgt ttaaacttag cctggcagca tatcgagatc gaagtcatcg agagcgtcag
     4741 cagggagcat atccagatca aaatcatcca gggcatcggc gggcagcatg tccaggtcga
     4801 agtcgtccag ggcgtcggtg ggtccgccgc tctcgcactt cagctgcttc tccaggccgc
     4861 agatgatcag ttccaggccg aacaggaagg cgggctcggc gccctgtctg tcgaacagct
     4921 cgatggcctg cttcagcagg gggggcatgc tgtcggtggt gggtgtctct ctttcctctt
     4981 tggcgacctg gtgttcctgt tcttccagca cgcagcccag ggtgaagtgg cccacggcgc
     5041 tcagggcgta cagggcgttt tccaggctga agccctgctg gcacaggaag gccagctggt
     5101 tttccagtgt ctcgtactgc ttctcggtgg gtctggtgcc caggtgcact ttggcgccgt
     5161 cccggtggct cagcagggcg catctgtagc tcttggcgtt gttccgcagg aagtcctgcc
     5221 agctctcgcc ttccagaggg cagctgtggg tgtggtgccg gtccagcatc tcgatgggca
     5281 gggcgtccag cagggcccgc ttgttcttca cgtgccagta cagggtgggc tgttccacgc
     5341 ccagtttctg ggccagcttc cgggtggtca ggccctcgat gcccacgccg ttcagcagtt
     5401 ccagggcgct gttgatcact ttgctcttgt ccagccggct catcgaccct ccgcctgggc
     5461 caggattctc ctcgacgtca ccgcatgtta gcagacttcc tctgccctcg gaaccgcctt
     5521 cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac
     5581 agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg
     5641 ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc
     5701 cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc
     5761 gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac
     5821 aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca
     5881 tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg
     5941 agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca
     6001 gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt
     6061 gatacacatg gggatcagca atcgcgcaga tgaaatcacg ccatgtagtg tattgaccga
     6121 ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg
     6181 catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt
     6241 gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg ctgaactccc
     6301 caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac
     6361 gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta
     6421 catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc
     6481 tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca
     6541 tggtaccggt cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact
     6601 actgcactta tatacggttc tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca
     6661 tcactttccc agtttacccc gcgccacctt ctctaggcac cggatcaatt gccgacccct
     6721 ccccccaact tctcggggac tgtgggcgat gtgcgctctg cccgtcgaca tatgggctag
     6781 cgcaccagac agtgacgtca gctgccagat cccatggccg tcatactgtg acgtctttca
     6841 gacaccccat tgacgtcaat gggagaacag atctggcctc ggcggccaag cttagacact
     6901 taggcgtgta cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc
     6961 tggagcaatt ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg
     7021 taaagaattc atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg
     7081 caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
     7141 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat
     7201 cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc
     7261 cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga
     7321 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
     7381 caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac
     7441 actcggctgg gaggccttca ccgagactct gtatcctgct gacggcggcc tggaaggcag
     7501 aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac
     7561 atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta
     7621 cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc
     7681 agtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatt aaacgcgtga
     7741 ttcgtcagta gggttgtaaa ggtttttctt ttcctgagaa aacaaccttt tgttttctca
     7801 ggttttgctt tttggccttt ccctagcttt aaaaaaaaaa aagcaaaact caccgaggca
     7861 gttccatagg atggcaagat cctggtattg gtctgcgagg atcctttctt tctttgaatt
     7921 tctactaagt gtagatactc gattcagtta acaagggtaa atttctacta agtgtagata
     7981 ctcgattcag ttaacaaggg taaatttcta ctaagtgtag atcgagtttt tttttgtttc
     8041 tcgagttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8101 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8161 cttatcatgt ctggatcaac tggataactc aagctaaccg actagtctag ttaaaaaatt
     8221 cgatatcaag cttatcgatc caaactggat ctctgctgtc cctgtaataa acccgaaaat
     8281 tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt
     8341 ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta aaattgtgga
     8401 tgaatactgc catttggcga taccgtcgag atccgttcac taatcgaatg gatctgtctc
     8461 tgtctctctc tccaccttct tcttctattc cttcgggcct gtcgggtccc ctcggggttg
     8521 ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa
     8581 gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg aataatttta
     8641 tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt
     8701 ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta
     8761 ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa
     8821 tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga
     8881 gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag
     8941 ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt
     9001 gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc
     9061 tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga
     9121 acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct
     9181 ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat ttatataatt
     9241 cacttctcca attgtccctc atatctcctc ctccaggtct gaagatcagc ggccggccgc
     9301 ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt
     9361 ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa
     9421 tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct
     9481 acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta gctccctgct
     9541 tgcccatact atatgtttta atttatattt tttctttccc cctggcctta accgaatttt
     9601 ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc
     9661 cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct
     9721 tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt
     9781 cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt
     9841 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
     9901 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
     9961 gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg cttatatgca
    10021 ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg gaaagtcccc
    10081 agcggaaagt cccttgtagc aagctcgata tcagcagttc ttgaagtact ccggatgcag
    10141 ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta acacttctct
    10201 ctccgggtca tccatcccat gcaggctcac agggtgtaac aagctggtgt tctctccttt
    10261 attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc atccaaaggt
    10321 cagtggatat ctgacccctg gccctggtgt gtagttctgc taatcaggga agtagccttg
    10381 tgtgtggtag atccacagat caaggatatc ttgtcttctt tgggagtgaa ttagcccttc
    10441 caactactaa gtttgtagta catatttaac aaatacaatt tctttaaaat gaaaataatt
    10501 cagaggaatc acaggtttag agtaaatgaa accacaggta attggcagtg gtaatagggt
    10561 atggggtggg aagtttggga tgattttggt tagcttgagt tatccagttg atccagacat
    10621 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
    10681 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
    10741 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
    10801 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tctagtcaag
    10861 gcactataca tcaaatattc cttattaacc cctttacaaa ttaaaaagct aaaggtacac
    10921 aatttttgag catagttatt aatagcagac actctatgcc tgtgtggagt aagaaaaaac
    10981 agtatgttat gattataact gttatgccta cttataaagg ttacagaata tttttccata
    11041 attttcttgt atagcagtgc agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga
    11101 gttctattac taaacacagc atgactcaaa aaacttagca attctgaagg aaagtccttg
    11161 gggtcttcta cctttctctt cttttttgga ggagtagaat gttgagagtc agcagtagcc
    11221 tcatcatcac tagatggcat ttcttctgag caaaacaggt tttcctcatt aaaggcattc
    11281 caccactgct cccattcatc agttccatag gttggaatct aaaatacaca aacaattaga
    11341 atcagtagtt taacacatta tacacttaaa aattttatat ttaccttaga gctttaaatc
    11401 tctgtaggta gtttgtccaa ttatgtcaca ccacagaagt aaggttcctt cacaaagatc
    11461 cggggcccac tcataaatcc agttgccgcc acggtagcca atcaccgtat cgtataaatc
    11521 atcgtcggta cgttcggcat cgctcatcac aatacgtgcc tggacgtcga ggatttcgcg
    11581 tgggtcaatg ccgcgccaga tccacatcag acggttaatc atgcgatacc agtgagggat
    11641 ggttttacca tcaagggccg actgcacagg cggttgtgcg ccgtgattaa agcggcggac
    11701 tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac agggtttcgc tcaggtttgc
    11761 ctgtgtcatg gatgcagcct ccagaatact tactggaaac tattgtaacc cgcctgaagt
    11821 taaaaagaac aacgcccggc agtgccaggc gttgaaaaga ttagcgaccg gagattggcg
    11881 ggacgaatac g
//
