LOCUS       sequence-361642-        6633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Turowec JP, Duncan JS, French AC, Gyenis L, St Denis NA, Vilk G, 
            Litchfield DW.
  TITLE     Protein kinase CK2 is a constitutively active enzyme that promotes 
            cell survival: strategies to identify CK2 substrates and manipulate 
            its activity in mammalian cells.
  JOURNAL   Methods Enzymol. 2010;484:471-93.
  PUBMED    21036246
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2010"; volume: "484"; pages: "471-93"
COMMENT     Sequence Label: pRS3 (CK2alpha, CK2beta)
FEATURES             Location/Qualifiers
     source          1..6633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(136..154,155..177,178..196,197..219,220..238,239..261,
                     262..280,281..303,304..322,323..345,346..364,365..387,
                     388..406)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        439..477
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     642..658
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             675..704
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          1431..2003
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(2135..2154)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2200..2255
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2254..2273)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2653..2670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2824..3412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2904..2923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3583..4374,4375..4443))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4206..4225
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4444..4548)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4616..4634
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    4997..5131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5034..5053)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5088..5107
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(5212..5301)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(6570..6586)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctgcaggaat tcggggccgc ggaggctgga tcggtcccgg tgtcttctat ggaggtcaaa
       61 acagcgtgga tggcgtctcc aggcgatctg acggttcact aaacgagctc tgcttatata
      121 ggtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
      181 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
      241 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
      301 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      361 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      421 gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt
      481 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      541 caccgggacc gatccagcct ccgcggcccc gaattcgagc tcggtacccg gggatcctct
      601 agtcagctga cgcgtgctag cgcggcctcg acgatggccg ctctagaact agtggatcca
      661 ccatggcgtc tatggaacaa aagctgatta gcgaagagga cctgaacaac gggaattctc
      721 gagcagccgc tgacgtgaag atgagcagct cagaggaggt gtcctggatt tcctggttct
      781 gtgggctccg tggcaatgaa ttcttctgtg aagtggatga agactacatc caggacaaat
      841 ttaatcttac tggactcaat gagcaggtcc ctcactatcg acaagctcta gacatgatct
      901 tggacctgga gcctgatgaa gaactggaag acaaccccaa ccagagtgac ctgattgagc
      961 aggcagccga gatgctttat ggattgatcc acgcccgcta catccttacc aaccgtggca
     1021 tcgcccagat gttggaaaag taccagcaag gagactttgg ttactgtcct cgtgtgtact
     1081 gtgagaacca gccaatgctt cccattggcc tttcagacat cccaggtgaa gccatggtga
     1141 agctctactg ccccaagtgc atggatgtgt acacacccaa gtcatcaaga caccatcaca
     1201 cggatggcgc ctacttcggc actggtttcc ctcacatgct cttcatggtg catcccgagt
     1261 accggcccaa gagacctgcc aaccagtttg tgcccaggct ctacggtttc aagatccatc
     1321 cgatggccta ccagctgcag ctccaagccg ccagcaactt caagagccca gtcaagacga
     1381 ttcgctgatt ccctcgaggg ggggccatct ctagagctga gaacttcagg gtgagtttgg
     1441 ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat ggagggggca
     1501 aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat ggaccctcat
     1561 gataattttg tttctttcac tttctactct gttgacaacc attgtctcct cttattttct
     1621 tttcattttc tgtaactttt tcgttaaact ttagcttgca tttgtaacga atttttaaat
     1681 tcacttttgt ttatttgtca gattgtaagt actttctcta atcacttttt tttcaaggca
     1741 atcagggtat attatattgt acttcagcac agttttagag aacaattgtt ataattaaat
     1801 gataaggtag aatatttctg catataaatt ctggctggcg tggaaatatt cttattggta
     1861 gaaacaacta caccctggtc atcatcctgc ctttctcttt atggttacaa tgatatacac
     1921 tgtttgagat gaggataaaa tactctgagt ccaaaccggg cccctctgct aaccatgttc
     1981 atgccttctt ctctttccta cagctcctgg gcaacgtgct ggttgttgtg ctgtctcatc
     2041 attttggcaa agaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg
     2101 tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg
     2161 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     2221 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     2281 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
     2341 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
     2401 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     2461 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     2521 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat
     2581 ccctcgactg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2641 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2701 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2761 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2821 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2881 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2941 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3001 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3061 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3121 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3181 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3241 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3301 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3361 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3421 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3481 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3541 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3601 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3661 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3721 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3781 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3841 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3901 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3961 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4021 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4081 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4141 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
     4201 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4261 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4321 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4381 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4441 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4501 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4561 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     4621 gcgtatcacg aggccctttc gtcttcactc gacgcggcca gcttggtcga gctggatact
     4681 tcccgtccgc cagggggaca tgccggcgat gctgaaggtc gcgcgcattc ccgatgaaga
     4741 ggccggttac cgcctgttga cctggtggga cgggcagggc gccgcccgag tcttcgcctc
     4801 ggcggcgggc gctctgctca tggagcgcgc gtccggggcc ggggaccttg cacagatagc
     4861 gtggtccggc caggacgacg aggcttgcag gatcataatc agccatacca catttgtaga
     4921 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     4981 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5041 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5101 actcatcaat gtatcttatc atgtctggat cctctagaac taggtcgggc cctctagatg
     5161 catgctcgag cggccgccag tgtgatggat atctgcagaa ttcggctttt aagcgtaatc
     5221 tggaacgtca tatggatagg atcctgcata gtccgggacg tcatagggat agcccgcata
     5281 gtcaggaaca tcgtatgggt actgctgagc gccagcggca gctggaacag gcatcccaag
     5341 ggggttggca gcagcaatca ctggtgagcc tgccagaggt ccaaggggtg aaggggttgg
     5401 cactgaagaa atccctgaca tcatattggc gctgctgacg ggcgtactgc cccctggcat
     5461 gctagatgaa cccattcgag cctggtcctt cacaacagtg tagaaatagg ggtgctccat
     5521 tgcctctctt gcagtaagcc gtgactggtg gtcatatcgc agcagtttgt ccaggaaatc
     5581 caaggcctca gggctgacaa ggtgctgatt ttcactgtgg acaaagcgtt cccatcgctt
     5641 tcgagagtgt ctgcccaaga tatcattgaa acgtggatct aattcaatgt tgtatttgtc
     5701 aatatagtca tataaatctt ctgtccccag aaccttggct atcctcacca actgatcata
     5761 attgtcatgt ccatggaaaa atggctcctt ccgaaagatc atacttgcca gcatacaacc
     5821 caaactccac atatccaaac tataatcgta catctgatag tctacaagta gctcaggacc
     5881 tttgaagtat cgggaagcaa ctcggacatt atattcttgg ccaggatgat aaaactcagc
     5941 caaaccccag tctattagtc gtagctttct gtgctcatga tcaatcatga cattatgggg
     6001 cttgacatct ctgtgcataa ttcccatgct gtgacaataa tccagggcct tcagaatctc
     6061 atacatgtaa aatcgaatat catagtctgt taacgtctgg tacaattgct tgaagtctgt
     6121 gttgtttacg tgttcaaaaa ccaaggcggg ggttcgtgac acagggtctt ttacaatgtc
     6181 tgccagtgtg atgatgttgg gacctcctct caaattctcc aaaatcttta tttcacgctt
     6241 aattttcttc ttttttactg gcttgagaat tttaacaaca actttttcat tatttgtgat
     6301 gttgatggct tcaaatactt cactgtattt acctcggcct aattttcgaa ccagctggta
     6361 gtcatcttga tttccccatt ccaccacatg tgactcgtaa tcccagtatt ctcgaggtct
     6421 gtgtgtatta acatctgtgt aaactctggc cctgcttggc acgggtcccg acatgtcaga
     6481 caggttggcg gacaaagctg gacttgatgt ttggagatct ggcagtcact gtgttcagaa
     6541 gcagctgggg gtaagacctt gtttcagagg atccactagt tctagagcgg ccgccaccgc
     6601 ggtggagctc ggtaccaagc ttcgacgcgg ccg
//
