LOCUS       sequence-361650-         956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Develop a family of genetically encoded fluorescent biosensors for 
            nicotinic agonists, termed iDrugSnFRs, thus enabling optical 
            subcellular pharmacokinetics for nicotinic agonists.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Nichols AL, Blumenfeld Z, Luebbert L, Knox HJ, Muthusamy AK, Marvin 
            JS, Kim CH, Grant SN, Walton DP, Cohen BN, Hammar R, Looger L, 
            Artursson P, Dougherty DA, Lester HA
  TITLE     Selective Serotonin Reuptake Inhibitors within Cells: Temporal 
            Resolution in Cytoplasm, Endoplasmic Reticulum, and Membrane.
  JOURNAL   J Neurosci. 2023 Mar 2:JN-RM-1519-22. doi: 
            10.1523/JNEUROSCI.1519-22.2022.
  PUBMED    36868853
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2023 Mar 2:JN-RM-1519-22. doi: 10.1523/JNEUROSCI.1519-22.2022."
COMMENT     Sequence Label: pAAV9-hSyn-iFluoxSnFR_cyto
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        185..633
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 gcgccgggca agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
       61 gcgcgcagag agggagtggc caactccatc actaggggtt ccttgtagtt aatgattaac
      121 ccgccatgct acttatctac gtagccatgc tctaggaaga tctctgcaga gggccctgcg
      181 tatgagtgca agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga
      241 ccgaccccga cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat
      301 cagagagggg gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac
      361 cgcggacagt gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag
      421 gcgcgctgac gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg
      481 tccgcgccgc cgccggccca gcccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcgnaggag tcgtgtcgtg cctganagcg cagtcgaatt caagctgcta gcaaggntcc
      661 cgccaccatg tctgcgancg acaccgtagt tgtgggctcg atcatcttta cagaagggnt
      721 tatcgtcgca aacatggngn cangagatga ttgangcgca tacagacnnt naaggnggtt
      781 cgcaanctga accttgncgg gttcaacgtt aactttganc cattaacgcg gangtgcgan
      841 aatggnattt gacattnacg tgnnnacact gggcacggtc tttgtgnnnt tntnggnntt
      901 nccnggagcc gangtctnnn tcaccgncga nngcnganna cggcatcang nnantt
//
