LOCUS       sequence-361651-        4036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Germline expression cloning vector for mosTI(unc-119). Transgene 
            inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: 
            aaaa - MCS - tgag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   El Mouridi S, Alkhaldi F, Frokjaer-Jensen C
  TITLE     Modular safe-harbor transgene insertion (MosTI) for targeted 
            single-copy and extrachromosomal array integration in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184.
  PUBMED    35900171
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184."
COMMENT     Sequence Label: pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2
FEATURES             Location/Qualifiers
     source          1..4036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      496..819
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(1178..1194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1178..1194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1191..1213)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1366..1954)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1446..1465)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2071..2095)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2195..2986,2987..3055))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2818..2837
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3056..3160)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3259..3281
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3273..3290
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3274..3290
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3914..3947)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aaaatacatt tgggcccacg gataaagaaa ttaaaaaaat acatttttta aaggcgcacc
       61 gaattaaaat tcatttgggt cttaccgcgt ataccgtact ccgtttgttt gatcattttt
      121 gtcagcgctg gcggttgttt tttcatttca tttctgcttc aaagacgttt tctcgaataa
      181 tttttcgttt attctctttt ttaaaattaa tttctagccg taaatgttat aaattcaccc
      241 atttaacgca aatttcatgg taatctcatg gaaaaatgca gtttctttgt taaagaaagc
      301 ttaaatagca aaaattcccc gactttcccc aaaatcctgc tcgattttcc gttttctcat
      361 tgtattctct cttaattaat tttatcgata atcaattgaa tgtttcagac agagaaaaat
      421 gagaccagct agctaagttt cgaactatct agatgaagct tttatcccat gcggtaccat
      481 attaggtctc ttgagatgca aaatcctttc aagcattccc ttcttctcta tcactcttct
      541 ttctttttgt caaaaaattc tctcgctaat ttatttgctt ttttaatgtt attattttat
      601 gactttttat agtcactgaa aagtttgcat ctgagtgaag tgaatgctat caaaatgtga
      661 ttctgtctga tgtactttca caatctctct tcaattccat tttgaagtgc tttaaacccg
      721 aaaggttgag aaaaatgcga gcgctcaaat atttgtattg tgttcgttga gtgacccaac
      781 aaaaagagga aactttattg tgccgccaag aaaaaagtct cacaggaaac ggagaatctg
      841 tgcggcaatg tcttatcatt tgaaacgcgc cgtatcgatg ctgacatcat gcggctcccg
      901 tgttagtcga gaagatgggt acgggccttt attcatccaa cactctacgg cttcttcaag
      961 agctagtagg gcaccctgta gttggaaagg gattatttcg tatggcgagc ccataccgtc
     1021 tttcttgcgg aagcttaaca cgataggaag ttggatagtt tcgaacgatg gttccatacg
     1081 gaacgctgtc tcaaggatga gtctgacgga gtgtaactcg aaggctaggt ggaggctcag
     1141 tgatgataag tctgcgatgg tggatgcatg tgtcatggtc atagctgttt cctgtgtgaa
     1201 attgttatcc gctcagaggg cacaatccta ttccgcgcta tccgacaatc tccaagacat
     1261 taggtggagt tcagttcggc gtatggcata tgtcgctgga aagaacatgt gagcaaaagg
     1321 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1381 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1441 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1501 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1561 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1621 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1681 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1741 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1801 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1861 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1921 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1981 gtctgacgct ctattcaaca aagccgccgt cccgtcaagt cagcgtaaat gggtaggggg
     2041 cttcaaatcg tcctcgtgat accaattcgg agcctgcttt tttgtacaaa cttgttgata
     2101 atggcaattc aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2161 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2221 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     2281 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagagc
     2341 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     2401 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     2461 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     2521 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     2581 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     2641 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     2701 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     2761 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     2821 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     2881 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     2941 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3001 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3061 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3121 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     3181 cagatacctg aaacaaaacc catcgtacgg ccaaggaagt ctccaataac tgtgatccac
     3241 cacaagcgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt catgcataat
     3301 ccgcacgcat ctggaataag gaagtgccat tccgcctgac ctaagtgctt ttcgagatcg
     3361 ccaaaccgga gaacgatcag gagaatgatg agtcgccaca ggagtcggca agatacgtgc
     3421 gttatagatt tgctccaaac tttttgaaac tcaaaacggt cggagcaact gtggaattca
     3481 aagtaggaga catcccaatc catcatttcc gaatgatcga acgtcacttc ttcaaagatc
     3541 gccttctgaa gtgttttgac tttgaattcg gattctgtat tccgaattca cgaaacaact
     3601 gtgaacatat ctatgagttc cctcaactct ctcaacaact cagtgagtca ttattctaaa
     3661 aagtacaatt caaaagacta atctcttttt cagtggacga catgatcaac aatccaaacg
     3721 agactcgttc tgacagcttc tatttcgtcg ataacaaact cgtcatgcac aacaaagccg
     3781 actactcata tgatgcataa atcttcattg ttgagtttat cttgttgatt tttgaataaa
     3841 ttatcaactc tttacttttt aatgggttat gaaataaata aacattgaaa actgataaac
     3901 aacgttcatc tctataactt cgtatagcat acattatacg aagttatggt tttcacaacg
     3961 gcaaaatatc agtttttaaa aaattaaacc ataaaacaaa taatataacc caatttttac
     4021 atcaaaccac aagaaa
//
