LOCUS       sequence-361655-        4140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitous expression cloning vector for mosTI(unc-119). Transgene 
            inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: 
            aaaa - MCS - tgag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   El Mouridi S, Alkhaldi F, Frokjaer-Jensen C
  TITLE     Modular safe-harbor transgene insertion (MosTI) for targeted 
            single-copy and extrachromosomal array integration in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184.
  PUBMED    35900171
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184."
COMMENT     Sequence Label: pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2
FEATURES             Location/Qualifiers
     source          1..4140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4..21
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5..21
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(645..678)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        679..1278
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     terminator      1367..1690
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(2049..2065)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2049..2065)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2062..2084)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2237..2825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2317..2336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2942..2966)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(3066..3857,3858..3926))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3689..3708
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3927..4031)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(4130..4140,1..12)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgttgtaaaa cgacggccag tcatgcataa tccgcacgca tctggaataa ggaagtgcca
       61 ttccgcctga cctaagtgct tttcgagatc gccaaaccgg agaacgatca ggagaatgat
      121 gagtcgccac aggagtcggc aagatacgtg cgttatagat ttgctccaaa ctttttgaaa
      181 ctcaaaacgg tcggagcaac tgtggaattc aaagtaggag acatcccaat ccatcatttc
      241 cgaatgatcg aacgtcactt cttcaaagat cgccttctga agtgttttga ctttgaattc
      301 ggattctgta ttccgaattc acgaaacaac tgtgaacata tctatgagtt ccctcaactc
      361 tctcaacaac tcagtgagtc attattctaa aaagtacaat tcaaaagact aatctctttt
      421 tcagtggacg acatgatcaa caatccaaac gagactcgtt ctgacagctt ctatttcgtc
      481 gataacaaac tcgtcatgca caacaaagcc gactactcat atgatgcata aatcttcatt
      541 gttgagttta tcttgttgat ttttgaataa attatcaact ctttactttt taatgggtta
      601 tgaaataaat aaacattgaa aactgataaa caacgttcat ctctataact tcgtatagca
      661 tacattatac gaagttatgc acctttggtc ttttattgtc aacttccatt ggttcttcca
      721 ttgtttctgt taaattaatg aatttttcat aaaataaaga cattatacaa tataaaaatg
      781 aagaatttat tgaaaataaa ctgccagaga gaaaaagtat gcaacactcc cgccgagagt
      841 gtttgaaatg gtgtacggta cattttcgtg ctaggagtta gatgtgcagg cagcaacgag
      901 agggggagag atttttttgg gccttgtgaa attaacgtga gttttctggt catctgacta
      961 atcatgttgg ttttttgttg gtttattttg tttttatctt tgtttttatc cagattagga
     1021 aatttaaatt ttatgaattt ataatgaggt caaacattca gtcccagcgt ttttcctgtt
     1081 ctcactgttt agtcgaattt ttattttagg ctttcaacaa atgttctaac tgtcttattt
     1141 gtgacctcac tttttatatt tttttaattt ttaaaaatat tagaagtttc taggataatt
     1201 ttttcgactt ttattctctc taccgtccgc actcttctta cttttaaatt aaattgtttt
     1261 tttttcagtt gggaaacact ttgcctaaaa tgagaccagc tagctaagtt tcgaactatc
     1321 tagatgaagc ttttatccca tgcggtacca tattaggtct cttgagatgc aaaatccttt
     1381 caagcattcc cttcttctct atcactcttc tttctttttg tcaaaaaatt ctctcgctaa
     1441 tttatttgct tttttaatgt tattatttta tgacttttta tagtcactga aaagtttgca
     1501 tctgagtgaa gtgaatgcta tcaaaatgtg attctgtctg atgtactttc acaatctctc
     1561 ttcaattcca ttttgaagtg ctttaaaccc gaaaggttga gaaaaatgcg agcgctcaaa
     1621 tatttgtatt gtgttcgttg agtgacccaa caaaaagagg aaactttatt gtgccgccaa
     1681 gaaaaaagtc tcacaggaaa cggagaatct gtgcggcaat gtcttatcat ttgaaacgcg
     1741 ccgtatcgat gctgacatca tgcggctccc gtgttagtcg agaagatggg tacgggcctt
     1801 tattcatcca acactctacg gcttcttcaa gagctagtag ggcaccctgt agttggaaag
     1861 ggattatttc gtatggcgag cccataccgt ctttcttgcg gaagcttaac acgataggaa
     1921 gttggatagt ttcgaacgat ggttccatac ggaacgctgt ctcaaggatg agtctgacgg
     1981 agtgtaactc gaaggctagg tggaggctca gtgatgataa gtctgcgatg gtggatgcat
     2041 gtgtcatggt catagctgtt tcctgtgtga aattgttatc cgctcagagg gcacaatcct
     2101 attccgcgct atccgacaat ctccaagaca ttaggtggag ttcagttcgg cgtatggcat
     2161 atgtcgctgg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2221 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2281 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2341 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2401 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2461 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2521 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2581 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2641 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2701 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2761 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2821 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tctattcaac aaagccgccg
     2881 tcccgtcaag tcagcgtaaa tgggtagggg gcttcaaatc gtcctcgtga taccaattcg
     2941 gagcctgctt ttttgtacaa acttgttgat aatggcaatt caaggatctt cacctagatc
     3001 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3061 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3121 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3181 ggccccagtg ctgcaatgat accgcgagag ccacgctcac cggctccaga tttatcagca
     3241 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3301 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3361 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3421 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3481 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3541 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3601 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3661 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3721 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3781 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3841 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3901 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3961 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4021 ggggttccgc gcacatttcc ccgaaaagtg ccagatacct gaaacaaaac ccatcgtacg
     4081 gccaaggaag tctccaataa ctgtgatcca ccacaagcgc cagggttttc ccagtcacga
//
