LOCUS       sequence-361660-       15177 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombinant rabies virus expressing histone-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15177)
  AUTHORS   Yao S, Wang Q, Hirokawa KE, Ouellette B, Ahmed R, Bomben J, Brouner 
            K, Casal L, Caldejon S, Cho A, Dotson NI, Daigle TL, Egdorf T, 
            Enstrom R, Gary A, Gelfand E, Gorham M, Griffin F, Gu H, Hancock N, 
            Howard R, Kuan L, Lambert S, Lee EK, Luviano J, Mace K, Maxwell M, 
            Mortrud MT, Naeemi M, Nayan C, Ngo NK, Nguyen T, North K, Ransford 
            S, Ruiz A, Seid S, Swapp J, Taormina MJ, Wakeman W, Zhou T, Nicovich
            PR, Williford A, Potekhina L, McGraw M, Ng L, Groblewski PA, Tasic 
            B, Mihalas S, Harris JA, Cetin A, Zeng H
  TITLE     A whole-brain monosynaptic input connectome to neuron classes in 
            mouse visual cortex.
  JOURNAL   Nat Neurosci. 2023 Feb;26(2):350-364. doi: 
            10.1038/s41593-022-01219-x. Epub 2022 Dec 22.
  PUBMED    36550293
REFERENCE   2  (bases 1 to 15177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-022-01219-x"; journalName: "Nat Neurosci"; date: 
            "2023-02"; volume: "26"; issue: "2"; pages: "350-364"
COMMENT     Sequence Label: AiP5001 RabV-CVS-N2cdeltaG-histone-EGFP
FEATURES             Location/Qualifiers
     source          1..15177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(77..868,869..937))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     700..719
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(938..1042)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1069..1525
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1156..1175)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1366..1387
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1651..1673
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1665..1682
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1666..1682
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        1752..2055
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2056..2259
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2210..2230
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2304..2323
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2304..2322
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5704..6081
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      6094..6103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6100..6102,6103..6105,6106..6819)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6100..6102,6103..6105,6106..6816)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6145..6166)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6406..6425)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6753..6774
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8994..9005
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(10797..10808)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(14004..14022)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      14018..14065
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(14093..14109)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14093..14109)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14106..14128)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14117..14133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14141..14147,14148..14165,14166..14171))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14186..14207
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14324..14341)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14495..15083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14575..14594)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
       61 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
      121 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
      181 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacccttg gaggctccag
      241 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
      301 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
      361 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
      421 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
      481 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
      541 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
      601 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
      661 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
      721 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
      781 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
      841 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
      901 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
      961 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     1021 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta
     1081 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     1141 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     1201 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     1261 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     1321 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     1381 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     1441 cgatttcggc ctattggtta aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     1501 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
     1561 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     1621 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     1681 gtgagcgcgc ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1741 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1801 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1861 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     1921 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     1981 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2041 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     2101 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     2161 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     2221 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
     2281 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
     2341 attaagcgtc tgatgagtcc gtgaggacga aacccggcgt accgggtcac gcttaacaac
     2401 aaaaccagag aagaaaaaga cagcgtcaat tgcaaagcaa aaatgtaaca cccctacaat
     2461 ggatgccgac aagattgtgt tcaaagtcaa taatcaggtg gtctctttga agcctgagat
     2521 tatcgtggat caatatgagt acaagtaccc tgccatcaag gatttgaaaa agccttgtat
     2581 caccctaggg aaagcccccg acttgaacaa agcatacaaa tcagttttat caggcatgaa
     2641 tgccgccaaa cttgatccgg atgatgtatg ctcctacttg gcagcagcaa tgcagttctt
     2701 tgaggggaca tgtccggaag actggaccag ctatggaatc ctgattgcac gaaaaggaga
     2761 taggatcacc ccaaactctc tagtggagat aaagcgtact gatgtagaag ggaattgggc
     2821 tctgacagga ggcatggaat tgacaaggga ccccactgtc tctgaacatg catctttagt
     2881 cggtcttctc ctgagtctgt acaggttgag caaaatatca ggacagaaca ctggtaacta
     2941 taagacaaac attgcagata ggatagagca gattttcgag acagcacctt ttgttaagat
     3001 cgtggaacac cataccctaa tgacaactca caagatgtgt gctaattgga gtactatacc
     3061 gaacttcaga tttttggccg gaacctacga catgtttttc tcacggattg agcatctata
     3121 ttcggcaatc agagtgggca cagtcgtcac cgcttatgaa gactgctcag gactggtatc
     3181 gtttacaggg ttcataaagc agatcaatct caccgcaagg gaagcaatac tatatttctt
     3241 ccacaagaac tttgaggaag agataagaag aatgttcgag ccagggcaag agacagctgt
     3301 tcctcactct tatttcatcc acttccgttc actaggcttg agtgggaagt ctccttattc
     3361 atcgaatgct gtcggtcatg tgttcaatct cattcacttt gttggatgct acatgggtca
     3421 agtcagatct ctaaatgcga cggttattgc tgcatgtgcc cctcatgaga tgtctgttct
     3481 agggggctat ttgggagagg aattcttcgg aaaagggaca tttgaaagaa ggttcttcag
     3541 agacgagaaa gaacttcaag aatatgaggc ggctgaacta acaaagtccg acgtggcact
     3601 ggcagatgac ggaaccgtca actctgatga cgaggactat ttctctggtg aaaccagaag
     3661 tccagaagct gtctatactc gaatcatgat gaatggaggt cgactgaaga gatctcatat
     3721 acggagatat gtctcagtca gttccaatca tcaagcccgt ccaaactcat tcgccgaatt
     3781 tttaaacaag acgtattcga atgactcata aggagttgat tgacagggtg ccagaaatct
     3841 atagattgta tatatccatc atgaaaaaaa ctaacactcc tcctttcaaa ccatcccaaa
     3901 tatgagcaag atctttgtta atccgagtgc aatcagagcc ggtctggccg atcttgagat
     3961 ggccgaagag actgttgatc tgatcaacag aaacatagaa gacaatcagg ctcatctcca
     4021 gggagaaccc atagaagtgg acaacctccc tgaggacatg aagcgacttc acctggacga
     4081 tgaaaaatcg tccaaccttg gtgagatggt tagggtggga gaaggcaagt atcgagagga
     4141 ctttcagatg gatgagggag aggaccccaa cctcctgttc caatcgtacc tggataatgt
     4201 tggagtccaa atagtcagac aaatgaggtc aggagagaga ttcctcaaga tatggtcaca
     4261 gaccgtagag gaaattgtat cctatgtcac ggtcaacttt cctaaccctc caagaaggtc
     4321 ttcggaggat aaatcaaccc agactactgg cagagagctc aagaaggaga caacgtctgc
     4381 tttctctcag agagaaagcc aaccttcgaa agctaggatg gtggctcaag ttgcccctgg
     4441 tcctccagcc cttgaatggt cagccaccaa tgaagaagat gatctatcag tagaggctga
     4501 gatcgctcat cagattgctg aaagcttttc caagaagtac aagtttccct cccgatcttc
     4561 aggaatattc ttgtataatt ttgagcaact gaagatgaac cttgatgaca tagttaaaga
     4621 ggcaaaaaat gtaccgggcg tgacccgtct ggcccatgat ggatccaaaa tccccctgag
     4681 atgcgtactg ggatgggtcg ctttggctaa ttccaaaaaa ttccaattac tagtcgaggc
     4741 tgacaagcta agcaaaatca tgcaagatga tttgaatcgc tatacatcct gctaaccgag
     4801 ttttcgaact cagtccctcc agataatgaa aactgagatg ttatggagta gacatgaaaa
     4861 aaacaggcaa caccactgat aaaatgaacg ttctacgcaa gatagtgaaa aaatgtaggg
     4921 atgaggacac tcaaaagccc tctcctgtgt cagcccctcc gtatgacgat gacctgtggc
     4981 ttccacctcc tgaatatgtc ccgctgaaag aactcacaag caagaagaac atgaggaact
     5041 tttgtgtcaa cggggaggtt aaagcgtgta gcccaaatgg ttactcattc aggattttgc
     5101 ggcacattct gagatcattc aacgagatat actctgggaa tcataggatg attgggttag
     5161 tcaaaattgt tgttggacta gctttatcag gagctccagt acctgagggc atgaactggg
     5221 tatacaaatt gaggagaacc cttatattcc agtgggctga ttccaggggc cctcttgaag
     5281 gggaggagtt agattactct caagagatca cttgggatga tgatactgaa ttcgtcggat
     5341 tgcaaataag agtgagcgca agacaatgtc atattcaagg caggatctgg tgtatcaaca
     5401 cgaactcgag ggcatgtcaa ctatggtctg acatgtctct tcagacacaa aggtctgaag
     5461 aggacaaaga ctcttctctg cttctagaat aatcagatta tatcctgcaa gtgtatcact
     5521 tgtttacctc tggaggagag agcatacagg cttgactccg atccttggga gcaatagaac
     5581 aaaaaaacac acgttatggt gccgttaaat cgctgcattt tatcaaagtc aagttgataa
     5641 cctttacatt ttgagcctct tggatgtgaa aaaaactatt aacatccctc aaaagacccc
     5701 gggatgccag agccagcgaa gtctgctccc gccccgaaaa agggctccaa gaaggcggtg
     5761 actaaggcgc agaagaaagg cggcaagaag cgcaagcgca gccgcaagga gagctattcc
     5821 atctatgtgt acaaggttct gaagcaggtc caccctgaca ccggcatttc gtccaaggcc
     5881 atgggcatca tgaattcgtt tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc
     5941 cgcctggcgc attacaacaa gcgctcgacc atcacctcca gggagatcca gacggccgtg
     6001 cgcctgctgc tgcctgggga gttggccaag cacgccgtgt ccgagggtac taaggccatc
     6061 accaagtaca ccagcgctaa ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
     6121 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     6181 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     6241 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     6301 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     6361 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     6421 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     6481 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     6541 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     6601 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     6661 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     6721 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     6781 gccgggatca ctctcggcat ggacgagctg tacaagtaag ctagcggcgc ctagccggtc
     6841 atccttttga cgattccagt cccgaggata acctcctctc gggattgggg ggaatctttg
     6901 gatccagtag tcctccttga actccgtcca acagggcaga ttcaagagtc ataagacttt
     6961 cattaatcat ttcagttgat cagacatggt cgtgtagatt ctcttaatac gggagatctt
     7021 ctagcagttt cagtgaccaa cggtgctttc attctccagg aactgatacc aaaggttgtg
     7081 gacaggccaa ggggtacttc ggatgactct gtgcttgggc acagaaagag gtcgtagtgt
     7141 gccccccgat agcggactcg acatgaatca actaagaaag gcaatctgcc tcccatgatg
     7201 gacataagca atagttcaca accatcttgc atctcagtga agtgtacata actattgagg
     7261 gctgggtcat ctaagcattt cagtcgagaa aaaaactgta gaccaaaaga acaactagca
     7321 acacttctca tccagagacc catatcaaga tgctagatcc gggagaggtt tatgatgacc
     7381 ctattgatcc aattgagtca gaggctgaac ccagaggaac ccccactgtc cccaacatct
     7441 tgaggaactc cgactacaat ctcaattctc ctttgataga ggatcctgcc aaactaatgt
     7501 tagaatggtt gaagacaggg aacagacctt atcggatgac tttgacagac aattgctcca
     7561 ggtcttacaa agttttgaaa gattatttca agaaagtaga tttgggttct ctcaaagtgg
     7621 gcggaactgc tgcacagtca atggtttctc tctggttgtg tggtgcccac tctgaatcaa
     7681 acaggagccg gagatgtata accgacttgg cccatttcta ttccaagtca tcccccatag
     7741 agaagctatt gaattgtacg ctaggaaaca gaggcctgag aatcccacca gagggggtgt
     7801 taaattgcct cgagagggtc aattatgaca aggcatttgg gaggtatctg gccaacacgt
     7861 attcctctta cttgtttttc catgtaatca ccttatacat gaatgcctta gactgggaag
     7921 aggaaaagac catcctggca ttatggaaag atataacctc agtggatacc gagaaggact
     7981 tggtcaaatt caaagatcaa atatggggac tgttgattgt gacaaaggac tttgtttact
     8041 ctcagagttc aaactgtctt tttgacagaa actacacact gatgctaaag gatcttttct
     8101 tgtctcgatt caactcctta atgattctgc tttctccccc tgagccccga tactcagatg
     8161 acttaatatc tcagctgtgc cagctataca tcgctgggga tcaagtcttg tccatgtgtg
     8221 ggaactccgg ctatgaagtc atcaaaatat tggagccata tgtcgtgaac agtttggtcc
     8281 agagggcaga gaagtttagg cctctcatcc accccttggg agactttcct atgttcataa
     8341 aagacaaggt gaatcaactt gaagggactt ttggtcccag tgcaaagagg ttttttaggg
     8401 ttttagatca attcgacaac atacatgact tagtatttgt gtatggctgt tacagacatt
     8461 gggggcaccc ctatatagat tatcggaagg gtctgtcgaa actatatgat caagttcaca
     8521 ttaagaaagt aatagataag tcctaccagg agtgtttagc aagtgacttg gccagaagga
     8581 tcctcagatg gggatttgac aagtactcca aatggtatct agattcgaga ttccttgccc
     8641 gagaccaccc cttgactcct tatgtcaaga cccaaacatg gccacccaaa catatagtag
     8701 acttggtggg ggacacatgg cataagctcc cgatcacgca gatctttgaa attcctgaat
     8761 caatggaccc gtcagagata ctggatgaca aatcacattc tttcaccaga acaaggttag
     8821 cttcttggct gtcagagaac cgaggggggc ctgttcctag cgagaaggtc attatcacgg
     8881 ccctgtctaa gccacctgtc aatccccgag agtttttgaa atctatagac ctcggaggat
     8941 tgccagatga ggatttgata attggcctca aaccaaagga acgggagttg aagatcgagg
     9001 gccgattctt tgctctaatg tcatggaatc taagattata ttttgtcatc accgaaaagc
     9061 tcttggccaa ctacattttg ccactttttg acgcactgac tatgacagac aacctgaaca
     9121 aggtgttcaa aaagttgatc gacagggtca ccgggcaagg gcttttggac tattctaggg
     9181 tcacatacgc atttcacctg gactatgaga aatggaacaa tcatcaaaga ttggagtcaa
     9241 cagaggatgt attctctgtc ctagatcagg tgtttggatt gaagagggtg ttttctagaa
     9301 cacacgagtt ttttcagaag tcctggatct attattcaga cagatcagac ctcattgggt
     9361 tatgggagga ccaaatatat tgcttggata tgtctaacgg cccaacctgc tggaatggcc
     9421 aagatggcgg gctagagggc ttgcggcaga agggctggag tctagtcagt ttattaatga
     9481 tagatagaga atctcaaacc aggaacacaa gaaccaagat actagctcaa ggagacaacc
     9541 aggttctgtg tcctacatac atgttgtcac cgggattgtc tcaagagggg cttctctatg
     9601 agttagagag catatcaagg aatgcactct caatataccg agctatcgag gaaggagcat
     9661 ctaagctggg gctgatcatc aagaaggaag agaccatgtg tagttatgac tttctcatat
     9721 atgggaagac ccccttattt cgaggcaaca tattggtgcc tgaatccaaa agatgggccc
     9781 gagtctcttg catctctaac gaccaaatag tcaacctcgc caatataatg tcgacagtat
     9841 ccaccaatgc gctgacagtg gcacaacact ctcaatctct gatcaaacct atgagggatt
     9901 ttctgctcat gtcagtacag gcagttttcc actacctgtt gtttagccca atcctaaaag
     9961 gcagagttta taagattctg agtgctgaag gggagagctt tctcctagcc atgtcgcgga
    10021 taatctacct agatccttct ttgggagggg tgtctggaat gtctctcggg aggttccata
    10081 tacgtcagtt ctcagaccct gtctctgaag ggttgtcatt ctggagagag atctggttag
    10141 gctctcatga gtcctggatt cacgcgttgt gtcaggaggc cgggaacccc gatcttggag
    10201 agagaacact cgagagcttc actcgccttt tagaagatcc tactacctta aatatcaaag
    10261 gaggggccag ccctaccatt ctactcaagg atgctatcag aaaggctctg tacgacgagg
    10321 tggacaaggt ggaaaattca gagtttcgag aggcaatcct gctgtccaag acccatagag
    10381 ataactttat actcttttta aaatctgttg agcctctgtt ccctcgattt ctcagtgagc
    10441 tcttcagttc gtctttcttg ggaataccgg agtcaatcat tggactgata caaaactccc
    10501 ggacaataag aaggcagttt agaaagagtc tctcaagaac tttagaagag tccttctaca
    10561 actcagagat ccacgggatt aatcggataa cccagacacc tcaaagggtc ggaagggtgt
    10621 ggccttgctc ttcagagagg gcagatctac ttagggagat ctcttggggg aggaaagtgg
    10681 taggcacgac agttcctcac ccttccgaga tgttggagtt gtttcccaaa tcctccattt
    10741 cctgcacttg tggagcaaca gggggaggca atcctagagt ctctgtatca gtactcccgt
    10801 ccttcgatca gtcatttttc tcacggggcc ccctaaaggg atacttgggc tcgtccacct
    10861 ccatgtcaac ccagctattc catgcatggg aaaaagtcac taatgttcat gtggtgaaaa
    10921 gggctctatc gttaaaagaa tctataaact ggttcatcaa taggaattcc aatttggctc
    10981 aaactctaat tggaaacatc atgtctctga caggccctga tttccctcta gaagaggccc
    11041 ctgttttcaa acggacaggg tcagccttgc ataggttcaa gtctgccaga tacagcgaag
    11101 gagggtattc ttctgtttgc cctaaccttc tctcccatat ctctgttagt acagacacta
    11161 tgtctgattt gacccaaaac gggaagaact atgatttcat gtttcagcca ttgatgcttt
    11221 atgcgcaaac atggacatcg gaactggtac agagggatac aagacttaga gactccacgt
    11281 ttcactggca ccttcggtgc aacagatgtg tgaggcccat tgatgatata acactggaaa
    11341 cttctcagat cttcgagttc ccggatgtgt caaaaaggat atccaggatg gtttctggag
    11401 ctgtccctca atttcagaag cttcctgata tccgtctaag accaggtgat tttgaatccc
    11461 taagtggtag agaaaagtct cgccatatag ggtcagctca ggggctctta tactcaatct
    11521 tagtagcaat tcacgactca ggatacaatg atgggaccat cttccctgtc aacatatacg
    11581 gcaaagtttc ccccagagac tatttgagag ggcttgcaag agggatcttg atagggtcct
    11641 cgatttgctt cttgacacga atgacaaata ttaacattaa cagacctctt gaattgatct
    11701 caggggtaat ttcctatatt ctcctgaggc tggataatca tccctctctg tatataatgc
    11761 ttagagaacc gtctcttaga ggagaaatat tctctatccc tcagaaaatc cccgccgctt
    11821 acccaaccac tatgagagaa ggcaacagat cgattttgtg ttacctccaa cacgtgctac
    11881 gctatgagcg agaggcaatc acggcgtccc cggagaatga ctggctgtgg atcttctcag
    11941 acttcagaag tgtgaaaatg acgtacttga ccctcattac ctaccagtct cacctcctac
    12001 tccagagggt tgagagaaac ttgtctaaga gtatgagagc tactctgcga caaatgagtt
    12061 ccttaatgag gcaagtgctg ggtgggcacg gagaagatac cttggagtca gacgatgaca
    12121 ttcaacgatt actaaaagac tctttgcgaa agacaaggtg ggtggatcaa gaggtgcgcc
    12181 atgcagctag aaccatgaat ggagattaca gccccgacaa gaaagtatcc cacaaggcag
    12241 gatgttcaga atgggtctgc tctgctcaac agattgccgt ctccacctca gccaacccgg
    12301 cccctgtctc agagcttgac attagggccc tctctaagag gtttcaaaac cccttgatct
    12361 cgggcctgag agtggttcag tgggcaaccg gtgcccatta taagcttaag cctattctag
    12421 atgatctaaa tgttttccca tctctctgtc ttgtaattgg agacgggtca gggggaatat
    12481 caagggcagt tctcaacatg tttccagatt ctaagcttgt gttcaacagc ctattggagg
    12541 tgaatgatct gatggcttcc ggaacacatc cactgcctcc ttcagcaatc atgagtggag
    12601 gagatgacat catctccaga gtgatagact ttgactcaat atgggagaaa ccgtccgacc
    12661 tgaggaactc ggccacctgg agatacttcc agtcggttca aaaacaggtc aacatgtcgt
    12721 atgacctcat tatttgtgat gcagaagtta ctgatattgc atctatcaac cggataactc
    12781 tgttgatgtc tgatttcgca ttgtctatag atggaccact ttatctggtc ttcaaaactt
    12841 acgggactat gctagtaaac ccggactata aagctattca acatctgtca agagcgttcc
    12901 cttcggtcac agggtttgta acccaagtaa cttcatcctt ttcttctgag ctatacctcc
    12961 ggttctctaa acgaggaaag tttttcaggg acgccgagta cttgacctct tccacccttc
    13021 gagagatgag ccttgtgttg ttcaattgca gcagccccaa aagtgagatg cagagagctc
    13081 gttccttaaa ctatcaagac ctggtaaggg gatttcctga agagatcata tcaaatcctt
    13141 acaacgaaat gatcataact ctgattgaca atgatgtaga gtccttccta gtccacaaga
    13201 tggtggatga tcttgagcta cagaggggaa ctctgtctaa agtggctatc attatatcca
    13261 tcatgatcgt tttttccaat agagtcttca acatttccaa acctttgact gaccccttgt
    13321 tctacccccc atctgatcct aaaatcctga ggcacttcaa catatgttgc agtactatga
    13381 tgtatctatc taccgcttta ggcgacgtcc ctaacttcgc aagacttcat gacctgtata
    13441 acagacctat aacttgttac ttcagaaaac aagttattcg agggaatatt tatctatctt
    13501 ggagttggtc cgatgatacc ccagtgttca agagagtagc ctgtaattct agcttgagtc
    13561 tgtcatctca ctggatcagg ttgatctaca agatagtgaa gactaccaga ctcattggca
    13621 gcataaaaga cctatcagga gaggtagaac gacatcttca tgggtataac agatggatca
    13681 ccctcgagga tatccgatct agatcatccc tactagatta cagttgtttg taagccggat
    13741 attaccgaaa gcctgtgcat gctaaaattc ttgtatgatg catcttgaaa aaaacaagat
    13801 cttgaatccg gacctctggt tgtttgattg ttttttccat ctttattgtt tttttgttaa
    13861 gcgtgggtcg gcatggcatc tccacctcct cgcggtccga cctgggcatc cgaaggagga
    13921 cgcacgtcca ctcggatggc taagggaggg cggggatccg gctgctaaca aagcccgaaa
    13981 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
    14041 taaacgggtc ttgaggggtt ttttgctgaa agtcgcgctt ggcgtaatca tggtcatagc
    14101 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    14161 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    14221 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    14281 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    14341 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    14401 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    14461 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    14521 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    14581 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    14641 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    14701 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    14761 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    14821 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    14881 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    14941 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    15001 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    15061 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    15121 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatct
//
