LOCUS       sequence-361661-        4478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet transcriptional reporter cloning vector for mosTI(unc-119).
            Transgene inserted upstream of mScarlet(2xNLS) and tbb-2 3' UTR. MCS
            or Golden Gate (BsaI: tgcc - MCS - aaaa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   El Mouridi S, Alkhaldi F, Frokjaer-Jensen C
  TITLE     Modular safe-harbor transgene insertion (MosTI) for targeted 
            single-copy and extrachromosomal array integration in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184.
  PUBMED    35900171
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184."
COMMENT     Sequence Label: pSEM389 - mosTI unc-119 - NNN - mScarlet(nls) ...
FEATURES             Location/Qualifiers
     source          1..4478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..31
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15..31
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(655..688)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             773..793
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      1715..2038
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(2397..2413)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2397..2413)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2410..2432)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2585..3173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2665..2684)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(3290..3314)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(3414..4205,4206..4274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4037..4056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4275..4379)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(4478,1..22)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ccagtcacga cgttgtaaaa cgacggccag tcatgcataa tccgcacgca tctggaataa
       61 ggaagtgcca ttccgcctga cctaagtgct tttcgagatc gccaaaccgg agaacgatca
      121 ggagaatgat gagtcgccac aggagtcggc aagatacgtg cgttatagat ttgctccaaa
      181 ctttttgaaa ctcaaaacgg tcggagcaac tgtggaattc aaagtaggag acatcccaat
      241 ccatcatttc cgaatgatcg aacgtcactt cttcaaagat cgccttctga agtgttttga
      301 ctttgaattc ggattctgta ttccgaattc acgaaacaac tgtgaacata tctatgagtt
      361 ccctcaactc tctcaacaac tcagtgagtc attattctaa aaagtacaat tcaaaagact
      421 aatctctttt tcagtggacg acatgatcaa caatccaaac gagactcgtt ctgacagctt
      481 ctatttcgtc gataacaaac tcgtcatgca caacaaagcc gactactcat atgatgcata
      541 aatcttcatt gttgagttta tcttgttgat ttttgaataa attatcaact ctttactttt
      601 taatgggtta tgaaataaat aaacattgaa aactgataaa caacgttcat ctctataact
      661 tcgtatagca tacattatac gaagttattg cctgagacca gctagcaagc ttgcatgcct
      721 gcaggtcgac tctagaggat ccccgggtac cgagctcagg tctctaaaaa tgcccaaaaa
      781 gaaacgaaag gtcgtttcta agggcgaagc cgtcatcaag gtaagtttta cgaaattctg
      841 aagctcacaa aacatggagt acatttttca ggagttcatg cggttcaagg tccacatgga
      901 gggtagtatg aacggacacg agttcgagat agagggagag ggagagggac ggccatacga
      961 gggaacccaa acggccaagc tcaaggtcac caaaggggga ccactcccat tctcctggga
     1021 tatcctctcc ccacagttca tgtacggaag tcgtgccttc accaagcacc ccgcaggtaa
     1081 gtttataatc tatgatctta atttggcaat ggaaaaagtt ttttcagata taccagatta
     1141 ctataagcag tccttcccag agggattcaa atgggagcgt gtcatgaact tcgaggatgg
     1201 aggagccgtc accgtcacgc aagacacgtc cctcgaggat ggaaccctaa tctacaaggt
     1261 aaagcttcgt ggaaccaact tccccccagg taagttttat tccataatac aaatatatat
     1321 tgaacaataa tgcttttcag atggaccagt catgcaaaag aaaactatgg gatgggaagc
     1381 ctcgaccgaa cgtctctacc cagaggacgg ggtcctcaaa ggagatatca agatggccct
     1441 aagactcaag gacggaggac ggtatcttgc cgatttcaag accacgtaca aggctaagaa
     1501 gccagtacaa atgccagggg cctacaacgt agaccgtaaa ctcgacatca cgtcccacaa
     1561 cgaggactac acggtcgtcg aacaatacga acgttccgaa ggacgtcact ccaccggagg
     1621 gatggacgag ctctacaagt cccggcgtcg taaggccaac ccaacgaagc tctccgagaa
     1681 cgccaaaaag ctcgccaaag aggtcgagaa ttaaatgcaa aatcctttca agcattccct
     1741 tcttctctat cactcttctt tctttttgtc aaaaaattct ctcgctaatt tatttgcttt
     1801 tttaatgtta ttattttatg actttttata gtcactgaaa agtttgcatc tgagtgaagt
     1861 gaatgctatc aaaatgtgat tctgtctgat gtactttcac aatctctctt caattccatt
     1921 ttgaagtgct ttaaacccga aaggttgaga aaaatgcgag cgctcaaata tttgtattgt
     1981 gttcgttgag tgacccaaca aaaagaggaa actttattgt gccgccaaga aaaaagtctc
     2041 acaggaaacg gagaatctgt gcggcaatgt cttatcattt gaaacgcgcc gtatcgatgc
     2101 tgacatcatg cggctcccgt gttagtcgag aagatgggta cgggccttta ttcatccaac
     2161 actctacggc ttcttcaaga gctagtaggg caccctgtag ttggaaaggg attatttcgt
     2221 atggcgagcc cataccgtct ttcttgcgga agcttaacac gataggaagt tggatagttt
     2281 cgaacgatgg ttccatacgg aacgctgtct caaggatgag tctgacggag tgtaactcga
     2341 aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca
     2401 tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat
     2461 ccgacaatct ccaagacatt aggtggagtt cagttcggcg tatggcatat gtcgctggaa
     2521 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2581 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2641 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2701 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2761 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2821 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2881 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2941 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3001 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3061 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3121 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3181 ctttgatctt ttctacgggg tctgacgctc tattcaacaa agccgccgtc ccgtcaagtc
     3241 agcgtaaatg ggtagggggc ttcaaatcgt cctcgtgata ccaattcgga gcctgctttt
     3301 ttgtacaaac ttgttgataa tggcaattca aggatcttca cctagatcct tttaaattaa
     3361 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3421 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3481 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3541 gcaatgatac cgcgagagcc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3601 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3661 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3721 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3781 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3841 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3901 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3961 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4021 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4081 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4141 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4201 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4261 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     4321 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4381 acatttcccc gaaaagtgcc agatacctga aacaaaaccc atcgtacggc caaggaagtc
     4441 tccaataact gtgatccacc acaagcgcca gggttttc
//
