LOCUS       sequence-361664-        4629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet co-expression cloning vector for mosTI(unc-119). Transgene 
            inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or 
            Golden Gate (BsaI: tgcc - MCS - tgag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   El Mouridi S, Alkhaldi F, Frokjaer-Jensen C
  TITLE     Modular safe-harbor transgene insertion (MosTI) for targeted 
            single-copy and extrachromosomal array integration in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184.
  PUBMED    35900171
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184."
COMMENT     Sequence Label: pSEM398 - mosTI unc-119 - NNN - operon - mScar...
FEATURES             Location/Qualifiers
     source          1..4629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..31
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15..31
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(655..688)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      1866..2189
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(2548..2564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2548..2564)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2561..2583)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2736..3324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2816..2835)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(3441..3465)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(3565..4356,4357..4425))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4188..4207
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4426..4530)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(4629,1..22)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ccagtcacga cgttgtaaaa cgacggccag tcatgcataa tccgcacgca tctggaataa
       61 ggaagtgcca ttccgcctga cctaagtgct tttcgagatc gccaaaccgg agaacgatca
      121 ggagaatgat gagtcgccac aggagtcggc aagatacgtg cgttatagat ttgctccaaa
      181 ctttttgaaa ctcaaaacgg tcggagcaac tgtggaattc aaagtaggag acatcccaat
      241 ccatcatttc cgaatgatcg aacgtcactt cttcaaagat cgccttctga agtgttttga
      301 ctttgaattc ggattctgta ttccgaattc acgaaacaac tgtgaacata tctatgagtt
      361 ccctcaactc tctcaacaac tcagtgagtc attattctaa aaagtacaat tcaaaagact
      421 aatctctttt tcagtggacg acatgatcaa caatccaaac gagactcgtt ctgacagctt
      481 ctatttcgtc gataacaaac tcgtcatgca caacaaagcc gactactcat atgatgcata
      541 aatcttcatt gttgagttta tcttgttgat ttttgaataa attatcaact ctttactttt
      601 taatgggtta tgaaataaat aaacattgaa aactgataaa caacgttcat ctctataact
      661 tcgtatagca tacattatac gaagttattg cctgagacca gctagcaagc ttgcatgcct
      721 gcaggtcgac tctagaggat ccccgggtac cgagctcagg tctcttgagc tgtctcatcc
      781 tactttcacc tagttaactg cttgtcttaa aatctatgct tctctttagt atctaaaatt
      841 ttcctagaag cttacaagta tataaattgt ctcttctcaa taaaggttgt atatttattc
      901 atcttattga atctgccatt tcctcgtttt tgcgagttta tataccttcc aattttcttt
      961 ctattgtatt ttcaacttct aattttaatt cagggaaact gcttcaacgc atcatggttt
     1021 ctaagggcga agccgtcatc aaggtaagtt ttacgaaatt ctgaagctca caaaacatgg
     1081 agtacatttt tcaggagttc atgcggttca aggtccacat ggagggtagt atgaacggac
     1141 acgagttcga gatagaggga gagggagagg gacggccata cgagggaacc caaacggcca
     1201 agctcaaggt caccaaaggg ggaccactcc cattctcctg ggatatcctc tccccacagt
     1261 tcatgtacgg aagtcgtgcc ttcaccaagc accccgcagg taagtttata atctatgatc
     1321 ttaatttggc aatggaaaaa gttttttcag atataccaga ttactataag cagtccttcc
     1381 cagagggatt caaatgggag cgtgtcatga acttcgagga tggaggagcc gtcaccgtca
     1441 cgcaagacac gtccctcgag gatggaaccc taatctacaa ggtaaagctt cgtggaacca
     1501 acttcccccc aggtaagttt tattccataa tacaaatata tattgaacaa taatgctttt
     1561 cagatggacc agtcatgcaa aagaaaacta tgggatggga agcctcgacc gaacgtctct
     1621 acccagagga cggggtcctc aaaggagata tcaagatggc cctaagactc aaggacggag
     1681 gacggtatct tgccgatttc aagaccacgt acaaggctaa gaagccagta caaatgccag
     1741 gggcctacaa cgtagaccgt aaactcgaca tcacgtccca caacgaggac tacacggtcg
     1801 tcgaacaata cgaacgttcc gaaggacgtc actccaccgg agggatggac gagctctaca
     1861 agtaaatgca aaatcctttc aagcattccc ttcttctcta tcactcttct ttctttttgt
     1921 caaaaaattc tctcgctaat ttatttgctt ttttaatgtt attattttat gactttttat
     1981 agtcactgaa aagtttgcat ctgagtgaag tgaatgctat caaaatgtga ttctgtctga
     2041 tgtactttca caatctctct tcaattccat tttgaagtgc tttaaacccg aaaggttgag
     2101 aaaaatgcga gcgctcaaat atttgtattg tgttcgttga gtgacccaac aaaaagagga
     2161 aactttattg tgccgccaag aaaaaagtct cacaggaaac ggagaatctg tgcggcaatg
     2221 tcttatcatt tgaaacgcgc cgtatcgatg ctgacatcat gcggctcccg tgttagtcga
     2281 gaagatgggt acgggccttt attcatccaa cactctacgg cttcttcaag agctagtagg
     2341 gcaccctgta gttggaaagg gattatttcg tatggcgagc ccataccgtc tttcttgcgg
     2401 aagcttaaca cgataggaag ttggatagtt tcgaacgatg gttccatacg gaacgctgtc
     2461 tcaaggatga gtctgacgga gtgtaactcg aaggctaggt ggaggctcag tgatgataag
     2521 tctgcgatgg tggatgcatg tgtcatggtc atagctgttt cctgtgtgaa attgttatcc
     2581 gctcagaggg cacaatccta ttccgcgcta tccgacaatc tccaagacat taggtggagt
     2641 tcagttcggc gtatggcata tgtcgctgga aagaacatgt gagcaaaagg ccagcaaaag
     2701 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2761 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2821 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2881 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2941 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3001 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3061 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3121 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3181 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3241 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3301 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3361 ctattcaaca aagccgccgt cccgtcaagt cagcgtaaat gggtaggggg cttcaaatcg
     3421 tcctcgtgat accaattcgg agcctgcttt tttgtacaaa cttgttgata atggcaattc
     3481 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3541 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3601 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3661 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagagc cacgctcacc
     3721 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3781 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3841 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3901 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3961 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4021 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4081 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4141 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4201 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4261 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4321 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4381 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4441 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4501 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cagatacctg
     4561 aaacaaaacc catcgtacgg ccaaggaagt ctccaataac tgtgatccac cacaagcgcc
     4621 agggttttc
//
