LOCUS       sequence-361670-       12439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12439)
  AUTHORS   Heanue TA, Johnson RL, Izpisua-Belmonte JC, Stern CD, De Robertis 
            EM, Tabin CJ
  TITLE     Goosecoid misexpression alters the morphology and Hox gene 
            expression of the developing chick limb bud.
  JOURNAL   Mech Dev. 1997 Dec . 69(1-2):31-7.
  PUBMED    9486529
REFERENCE   2  (bases 1 to 12439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mech Dev. 
            1997 Dec . 69(1-2):31-7."
COMMENT     Sequence Label: RCAS (A) goosecoid (CT#19)
FEATURES             Location/Qualifiers
     source          1..12439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7980..8241
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(8831..8853)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8842..8858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8866..8872,8873..8890,8891..8896))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(8939..8957)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9025..9129
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9130..9198,9199..9990)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9348..9367)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10161..10749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10650..10669
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10903..10920
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10935..11075
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11001..11020)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11161..11183
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(11177..11368)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        join(12211..12439,1..33)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gccatttgac cattcaccac attggtgtgc acctgggttg atggccggac cgttgattcc
       61 ctgacgacta cgagcacctg catgaagcag aaggcttcat ttggtgaccc cgacgtgata
      121 gttagggaat agtggtcggc cacagacggc gtggcgatcc tgtctccatc cgtctcgtct
      181 atcgggaggc gagttcgatg accctggtgg agggggctgc ggcttaggga ggcagaagct
      241 gagtaccgtc ggagggagct ccagggcccg gagcgactga cccctgccga gaactcagag
      301 ggtcgtcgga agacggagag tgagcccgac gaccacccca ggcacgtctt tggtcggcct
      361 gcggatcaag catggaagcc gtcattaagg tgatttcgtc cgcgtgtaaa acctattgcg
      421 ggaaaatctc tccttctaag aaggaaatag gggccatgtt gtccctgtta caaaaggaag
      481 ggttgcttat gtctccctca gatttatatt ctccggggtc ctgggatccc atcactgcgg
      541 cgctctccca gcgggcaatg gtacttggaa aatcgggaga gttaaaaacc tggggattgg
      601 ttttgggggc attgaaggcg gctcgagagg aacaggttac atctgagcaa gcaaagtttt
      661 ggttgggatt agggggaggg agggtctctc ccccaggtcc ggagtgcatc gagaaaccag
      721 ctacggagcg gcgaatcgac aaaggggagg aggtgggaga aacaactgtg cagcgagatg
      781 cgaagatggc gccagaggaa gcggccacac ctaaaaccgt tggcacatcc tgctatcatt
      841 gcggaacagc tgttggctgc aattgcgcca ccgccacagc ctcggcccct cctccccctt
      901 atgtggggag tggtttgtat ccttccctgg cgggggtggg agagcagcag ggccagggag
      961 ataacacgtc tcggggggcg gagcagccaa gggaggagcc agggcacgcg ggtcaggccc
     1021 ctgggccggc cctgactgac tgggcaaggg taagggagga gcttgcgagt actggtccgc
     1081 ccgtggtggc catgcctgta gtgattaaga cagagggacc cgcctggacc cctctggagc
     1141 caaaattgat cacaagactg gctgatacgg tcaggaccaa gggcttacga tccccgatca
     1201 ctatggcaga agtggaagcg ctcatgtcct ccccgttgct gccgcatgac gtcacgaatc
     1261 taatgagagt gattttagga cctgccccat atgccttatg gatggacgct tggggagtcc
     1321 aactccagac ggttatagcg gcagccactc gcgacccccg acacccagcg aacggtcaag
     1381 ggcgggggga acggactaac ttggatcgat taaagggctt agctgatggg atggtgggca
     1441 acccacaggg tcaggccgca ttattaagac cgggggaatt ggttgctatt acggcgtcgg
     1501 ctctccaggc gtttagagaa gttgcccggc tggcggaacc tgcaggtcca tgggcggaca
     1561 tcacgcaggg accatctgag tcctttgttg attttgccaa tcggcttata aaggcggttg
     1621 aggggtcaga tctcccgcct tccgcgcggg ctccggtgat cattgactgc tttaggcaga
     1681 agtcacagcc agatattcag cagcttatac gggcagcacc ctccacgctg accaccccag
     1741 gagagataat caaatatgtg ctagacaggc agaagattgc ccctcttacg gatcaaggca
     1801 tagccgcggc catgtcgtct gctatccagc ccttagttat ggcagtagtc aatagagaga
     1861 gggatggaca aactgggtcg ggtggtcgtg cccgagggct ctgctacact tgtggatccc
     1921 cgggacatta tcaggcacag tgcccgaaaa aacgaaagtc aggaaacagc cgtgagcgat
     1981 gtcagctgtg tgacgggatg ggacacaacg ctaaacagtg taggaagcgg gatggcaacc
     2041 agggccaacg cccaggaaga ggtctctctt cggggccgtg gcccggccct gagcagcctg
     2101 ccgtctcgtt agcgatgaca atggaacata aagatcgccc cttggttagg gtcattctga
     2161 ctaacactgg gagtcatcca gtcaaacaac gttcggtgta tatcaccgcg ctgttggact
     2221 ccggagcgga catcactatt atttcggagg aggattggcc tactgattgg ccggtggtgg
     2281 acaccgcgaa cccacagatc catggcatag gagggggaat tcccatgcga aaatcccggg
     2341 atatgataga ggtgggggtt attaaccgag acgggtcgtt ggagcgaccc ctgctcctct
     2401 tccccgcagt cgctatggtt agagggagta tcctaggaag agattgtctg cagggcctag
     2461 ggctccgctt gacaaattta tagggagggc cactgttctt actgttgcgc tacatctggc
     2521 tattccgctc aaatggaagt cagaccgcac gcctgtgtgg attgaccagt ggccccttcc
     2581 tgaaggtaaa cttgtagcgc taacgcaatt agtggaaaaa gaattacagt taggacatat
     2641 agagccctca cttagttgtt ggaacacacc tgtttttgtg atccggaagg cttccgggtc
     2701 ttatcgctta ttgcatgatt tgcgcgctgt taacgccaag cttgttcctt ttggggccgt
     2761 ccaacagggg gcgccagttc tctccgcgct cccgcgtggc tggcccctga tggtcctaga
     2821 cctcaaggat tgcttctttt ctatccctct tgcggaacaa gatcgcgaag cttttgcatt
     2881 tacgctcccc tctgtgaata accaggcccc cgctcgaaga ttccaatgga aggtcttgcc
     2941 ccaagggatg acctgttctc ccactatctg tcagttggta gtgggtcagg tgctcgagcc
     3001 cttgcgactc aagcacccag ctctgcgcat gttgcattat atggacgatc ttttgctagc
     3061 cgcctcaagt catgatgggt tggaagcggc agggaaggag gttatcggta cattggaaag
     3121 agccgggttc actatttcgc cggataagat ccagagggag cccggagtac aatatcttgg
     3181 gtacaagtta ggcagtacgt atgtagcacc cgtaggcttg gtagcagaac ccaggatagc
     3241 caccttgtgg gatgttcaaa agctggtggg gtcacttcag tggcttcgcc cagcgttagg
     3301 gatcccgcca cgactgatgg gtccctttta tgagcagtta cgagggtcag atcctaacga
     3361 ggcgagggaa tggaatctag acatgaaaat ggcctggaga gagatcgtac agcttagcac
     3421 tactgctgcc ttggaacgat gggaccctgc ccagcctctg gaaggagcgg tcgctagatg
     3481 tgaacagggg gcaatagggg tcctgggaca gggactgtcc acacacccaa ggccatgttt
     3541 gtggttattc tccacccaac ccaccaaggc gtttactgct tggttagaag tgctcaccct
     3601 tttgattact aagctacgcg cttcggcagt gcgaaccttt ggcaaggagg ttgatatcct
     3661 cctgttgcct gcatgcttcc gggaggacct tccgctcccg gaggggatcc tgttagcact
     3721 tagggggttt gcaggaaaaa tcaggagtag tgacacgcca tctatttttg acattgcgcg
     3781 tccactgcat gtttctctga aagtgagggt taccgaccac cctgtgccgg gacccactgt
     3841 ctttaccgac gcctcctcaa gcacccataa aggggtggta gtctggaggg agggcccaag
     3901 gtgggagata aaagaaatag ttgatttggg ggcaagtgta caacaactgg aggcacgcgc
     3961 tgtggccatg gcacttctgc tgtggccgac aacgcccact aatgtagtga ctgactctgc
     4021 gtttgttgcg aaaatgttac tcaagatggg acaggaggga gtcccgtcta cagcggcagc
     4081 ttttatttta gaggatgcgt taagccaaag gtcagccatg gccgccgttc tccacgtgcg
     4141 gagtcattct gaagtgccag ggtttttcac agaaggaaat gacgtggcag atagccaagc
     4201 cacctttcaa gcgtatccct tgagagaggc taaagatctt cataccgctc tccatattgg
     4261 accccgcgcg ctatccaaag cgtgtaatat atctatgcag caggctaggg aggttgttca
     4321 gacctgcccg cattgtaatt cagcccctgc gttggaggcc ggggtaaacc ctaggggttt
     4381 gggaccccta cagatatggc agacagactt tacgcttgag cctagaatgg ctccccgttc
     4441 ctggctcgct gttactgtgg acaccgcctc atcagcgata gtcgtaactc agcatggccg
     4501 tgttacatcg gttgctgcac aacatcattg ggccacggct atcgccgttt tgggaagacc
     4561 aaaggccata aaaacagata acgggtcctg cttcacgtct aaatccacgc gggagtggct
     4621 cgcgagatgg gggatagcac acaccaccgg gattccggga aattcccagg gtcaagctat
     4681 ggtagagcgg gccaaccggc tcctgaaaga taagatccgt gtgcttgcgg aaggggacgg
     4741 ctttatgaaa agaatccccg ccagcaaaca gggggaacta ctagccaaag caatgtatgc
     4801 cctcaatcac tttgagcgtg gtgaaaacac aaaaacaccg gtacaaaaac actggagacc
     4861 taccgttctt acagaaggac ccccggttaa aatacgaata gagacagggg agtgggaaaa
     4921 aggatggaac gtgctggtct ggggacgagg ttatgccgct gtgaaaaaca gggacactga
     4981 taaggttatt tgggtaccct ctcggaaagt taaaccggat gtcacccaaa aggatgaggt
     5041 gactaagaaa gatgaggcga gccctctttt tgcaggcatt tctgactgga taccctggga
     5101 agacgagcaa gaaggactcc aaggagaaac cgctagcaac aagcaagaaa gacccggaga
     5161 agacaccctt gctgccaacg agagttaatt atattctcat tattggtgtc ctggtcttgt
     5221 gtgaggttac gggggtaaga gctgatgtcc acttactcga gcagccaggg aacctttgga
     5281 ttacatgggc caaccgtaca ggccaaacgg atttttgcct ctctacacag tcagccacct
     5341 ccccttttca aacatgtttg ataggtatcc cgtcccctat ttccgagggt gattttaagg
     5401 gatatgtttc tgatacaaat tgcaccacct tgggaactga tcggttagtc tcgtcagccg
     5461 actttactgg cggacctgac aacagtacca ccctcactta tcggaaggtc tcatgcttgt
     5521 tgttaaagct gaatgtctct atgtgggatg agccacctga actacagctg ttaggttccc
     5581 agtctctccc taacattact aatattgctc agatttccgg tataaccggg ggatgcgtag
     5641 gcttcagacc acaaggggtt ccttggtatc taggttggtc tagacaggag gccacgcggt
     5701 ttctccttag acacccctct ttctctaaat ccacggaacc gtttacggtg gtgacagcgg
     5761 ataggcacaa tctttttatg gggagtgagt actgcggtgc atatggctac agattttgga
     5821 acatgtataa ctgctcacag gtggggcggc agtaccgctg tggtaatgcg cgcagccccc
     5881 gcccgggtct tcctgaaatc cagtgtacaa ggagaggagg caaatgggtt aatcaatcac
     5941 aggaaattaa tgagtcggag ccgttcagct ttacggtgaa ctgtacagct agtagtttgg
     6001 gtaatgccag tgggtgttgc ggaaaagcag gcacgattct cccgggaaag tgggtcgaca
     6061 gcacacaagg tagtttcacc aaaccaaaag cgctaccacc cgcaattttc ctcatttgtg
     6121 gggatcgcgc atggcaagga attcccagtc gtccggtagg gggcccctgc tatttaggca
     6181 agcttaccat gttagcacct aagcatacag atattctcaa ggtgcttgtc aattcatcgc
     6241 ggacaggtat aagacgtaaa cgaagcacct cacacctgga tgatacatgc tcagatgaag
     6301 tgcagctttg gggtcctaca gcaagaatct ttgcatctat cctagccccg ggggtagcag
     6361 ctgcgcaagc cttaagagaa attgagagac tagcctgttg gtccgttaaa caggctaact
     6421 tgacaacatc actcctcggg gacttattgg atgatgtcac gagtattcga cacgcggtcc
     6481 tgcagaaccg agcggctatt gacttcttgc tcctagctca cggccatggc tgtgaggacg
     6541 ttgccggaat gtgctgtttc aatttgagtg atcacagtga gtctatacag aagaagttcc
     6601 agctaatgaa ggaacatgtc aataagatcg gcgtggacag cgacccaatt ggaagttggc
     6661 tgcgaggact attcggggga ataggagaat gggccgttca tttgctgaaa ggactgcttt
     6721 tggggcttgt agttattttg ttgctagtag tgtgcctgcc ttgccttttg caaatcgtat
     6781 gcggtaacat cagaaagatg attaataact ccatcagcta ccacacggaa tataagaagc
     6841 tgcaaaaggc ctgtgggcag cctgaaagca gaatagtata aggcagtaca tgggtggtgg
     6901 tatagcgctt gcgagtccat cgaggaaggc aggaaagaca gctattggta attgtgaaat
     6961 acgcttttgt ctgtgtgctg caggagctga gctgactctg ctggtggcct cgcgtaccac
     7021 tgtggcatcg attctagacc actgtggcca ggcggtagct gggacgtgca gccgaccacc
     7081 atggctgcga gcatgttcag catcgacaac atcctggcgg ccagacctcg ctgcaaggac
     7141 tcggtgctgc tgcccccgag cgccgcgccc gtcgtcttcc ccagcctgca cggggactcc
     7201 ctctacggcg ctgcctccga ctacggagga ttttactccc gggctgtggc tcccggctcg
     7261 gcgctgccgg cggtcggcgg ctcccgcctg ggctacaaca actactacta cgggcagctg
     7321 catgtgccca cgtctcccgt gggcccgtcg tgttgcgggg ccgtgccgcc gctgggggcc
     7381 cagcagtgct cctgcgtgcc ccccgcaggt tacgagggcg ctgggtcggt gctgatgtcc
     7441 cctgttcccc atcagatgtt gccctacatg aacgtaggca ctttgtcccg gacggagctg
     7501 cagttactca accagctgca ctgcaggcgg aaaagacggc accggactat cttcactgac
     7561 gagcagctcg aagcgctgga aaacctcttc caggaaacga aatacccaga cgtgggcacc
     7621 agggaacagc tggcgaggag ggtgcactta agagaggaga aagtggaggt ttggttcaaa
     7681 aaccgccggg cgaaatggag gaggcaaaag cggtcgtctt ccgaggagtc ggaaaatgca
     7741 cagaaatgga ataaagcgtc taaaacgtct ccggagaaga ggcaagaaga cgggaaaagc
     7801 gatttggact ccgacagctg agtcgacctg cagccaagct tatcgatgcg atgtacgggc
     7861 cagatatacg cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt
     7921 gtacgcggtt aggagtcccc tcaggatata gtagtttcgc ttttgcatag ggagggggaa
     7981 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     8041 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     8101 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattc
     8161 cgcattgcag agatattgta tttaagtgcc tagctcgata caataaacgc catttgacca
     8221 ttcaccacat tggtgtgcac ctgggttgat ggccggaccg ttgattccct gacgactacg
     8281 agcacctgca tgaagcagaa ggcttcattt ggtgaccccg acgtgatagt tagggaatag
     8341 tggtcggcca cagacggcgt ggcgatcctg tctccatccg tctcgtctat cgggaggcga
     8401 gttcgatgac cctggtggag ggggctgcgg cttagggagg cagaagctga gtaccgtcgg
     8461 aggggatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta
     8521 gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg
     8581 cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc
     8641 tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc
     8701 ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac
     8761 acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agctccaagc
     8821 ttggctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacatta tacgagccgg
     8881 aagcataaag tgtaaagcct ggggtgccta atgagtgaga attcttgaag acgaaagggc
     8941 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     9001 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     9061 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     9121 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     9181 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     9241 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     9301 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     9361 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     9421 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     9481 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     9541 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     9601 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     9661 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9721 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9781 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9841 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9901 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9961 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    10021 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    10081 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    10141 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    10201 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    10261 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
    10321 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    10381 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    10441 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    10501 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    10561 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10621 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    10681 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10741 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    10801 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    10861 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    10921 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
    10981 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
    11041 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
    11101 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
    11161 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
    11221 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
    11281 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
    11341 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggagga tttctgttca
    11401 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg
    11461 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg
    11521 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc
    11581 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg
    11641 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
    11701 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
    11761 tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga
    11821 tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg
    11881 agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc
    11941 gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc
    12001 ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac
    12061 aaggtatagg gcggcgatgc gatgtacggg ccagatatac gcgtatctga ggggactagg
    12121 gtgtgtttag gcgaaaagcg gggcttcggt tgtacgcggt taggagtccc ctcaggatat
    12181 agtagtttcg cttttgcata gggaggggga aatgtagtct tatgcaatac tcttgtagtc
    12241 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
    12301 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
    12361 tctgacatgg attggacgaa ccactgaatt ccgcattgca gagatattgt atttaagtgc
    12421 ctagctcgat acaataaac
//
