LOCUS       sequence-361686-        5038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene
            inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. 
            MCS or Golden Gate (BsaI: aaaa - MCS - tgag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   El Mouridi S, Alkhaldi F, Frokjaer-Jensen C
  TITLE     Modular safe-harbor transgene insertion (MosTI) for targeted 
            single-copy and extrachromosomal array integration in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184.
  PUBMED    35900171
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184."
COMMENT     Sequence Label: pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2
FEATURES             Location/Qualifiers
     source          1..5038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(639..672)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      2259..2582
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(2941..2957)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2941..2957)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2954..2976)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(3129..3717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3209..3228)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(3834..3858)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(3958..4749,4750..4818))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASQQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4581..4600
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4819..4923)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(5022..5038,1..6)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(5036..5038,1..15)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(5037..5038,1..15)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaaacgacgg ccagtcatgc ataatccgca cgcatctgga ataaggaagt gccattccgc
       61 ctgacctaag tgcttttcga gatcgccaaa ccggagaacg atcaggagaa tgatgagtcg
      121 ccacaggagt cggcaagata cgtgcgttat agatttgctc caaacttttt gaaactcaaa
      181 acggtcggag caactgtgga attcaaagta ggagacatcc caatccatca tttccgaatg
      241 atcgaacgtc acttcttcaa agatcgcctt ctgaagtgtt ttgactttga attcggattc
      301 tgtattccga attcacgaaa caactgtgaa catatctatg agttccctca actctctcaa
      361 caactcagtg agtcattatt ctaaaaagta caattcaaaa gactaatctc tttttcagtg
      421 gacgacatga tcaacaatcc aaacgagact cgttctgaca gcttctattt cgtcgataac
      481 aaactcgtca tgcacaacaa agccgactac tcatatgatg cataaatctt cattgttgag
      541 tttatcttgt tgatttttga ataaattatc aactctttac tttttaatgg gttatgaaat
      601 aaataaacat tgaaaactga taaacaacgt tcatctctat aacttcgtat agcatacatt
      661 atacgaagtt atctgtgtct ctccgtccta tccactatct ttcatccaga ttcccggttt
      721 tcccgagaaa aagtgagaga gagaagaggc tactgcgcgg cgacactggc tgcggacacc
      781 ctcactcctc tacttgtctc catcgatttt cgctggttcg tcgaagcctc gtcgccaaca
      841 caacttcttc ttctttttcg tcctcttctt ttctgccaca cgcttgcaca gcttcttctt
      901 ctcattttac gctgtctact tttttcggag gggccatacc cccacacaca gctcctttta
      961 tttggcttct cttcttcttc ttgtccatat acttcttaaa agtctcgaac ttctctctct
     1021 cactctccat caatttcttt atggctaccg cttgcgctct gccgcttccg aagaaagaag
     1081 gcgttggcag agctctcaat tctatttttt ctcgccgtag gctatcgatt tctcagcttc
     1141 tcttctctct tcctcactcc accaccacca cttcgggctt cttcttttct tcattttcgt
     1201 cttcttcttc atcctcttct ttttttttca gttccccctc actcctcccc ttatatgcgc
     1261 gtgcgagagg ggtgcaaaag cagcgcgcat ccgagaattg aaacgagaaa acggagacgc
     1321 agcagcagtt cgtcctcaga aaaatagcca gtaaaaagag aaaaagatag agatagacct
     1381 atcgcatttt attttcaaat tgcaattcct gcaattcatg tgtgcgtgtg cccattttca
     1441 attcttcccg ttatttttca gaccattacc aacgttcttt ctagatcttg agacaatctt
     1501 ccttctgctc aatcgttcgt cgtgaagacg gcatcgacga cgacgacgta gagggccggg
     1561 actcttccca ctgaaactca atacttttgc atatttcaca ttctcttcct ccgttggttc
     1621 accaaaagag aaaagggacc gacatgaaaa ggaaacgaaa gcttaaaaga gacggaaaat
     1681 cgatgtacta gagtgctcgg aagtagttga gaggcgcaga gagagagctt tcattcagaa
     1741 gacacacgac gttattcctc gcaacgcctt cttcttttta acaatcttca atacatttta
     1801 gtgaactttc ttttatttct gtttcattta aaaatagtct taaatttcac tatatttaat
     1861 ttttttcttt tattatctgg aatgtttcag ctttcaacat gccattaaag agtgtgctcc
     1921 acgagccgga gagacgcaga tgggcatgag aagaagtcga taagaaagga gggtgccgtg
     1981 ctccctcaac gtcgtctgct ccatcgccga atttgtgctc tttcgtcgag agagctcttc
     2041 actttgaact ggccaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
     2101 atactaacat aacatagaac attttcagga ggacccttgg aggtgtaccg gttttcccag
     2161 tcacgacgcg tggtacgagc tcagaaaatg agaccagcta gctaagtttc gaactatcta
     2221 gatgaagctt ttatcccatg cggtaccggt ctcttgagat gcaaaatcct ttcaagcatt
     2281 cccttcttct ctatcactct tctttctttt tgtcaaaaaa ttctctcgct aatttatttg
     2341 cttttttaat gttattattt tatgactttt tatagtcact gaaaagtttg catctgagtg
     2401 aagtgaatgc tatcaaaatg tgattctgtc tgatgtactt tcacaatctc tcttcaattc
     2461 cattttgaag tgctttaaac ccgaaaggtt gagaaaaatg cgagcgctca aatatttgta
     2521 ttgtgttcgt tgagtgaccc aacaaaaaga ggaaacttta ttgtgccgcc aagaaaaaag
     2581 tctcacagga aacggagaat ctgtgcggca atgtcttatc atttgaaacg cgccgtatcg
     2641 atgctgacat catgcggctc ccgtgttagt cgagaagatg ggtacgggcc tttattcatc
     2701 caacactcta cggcttcttc aagagctagt agggcaccct gtagttggaa agggattatt
     2761 tcgtatggcg agcccatacc gtctttcttg cggaagctta acacgatagg aagttggata
     2821 gtttcgaacg atggttccat acggaacgct gtctcaagga tgagtctgac ggagtgtaac
     2881 tcgaaggcta ggtggaggct cagtgatgat aagtctgcga tggtggatgc atgtgtcatg
     2941 gtcatagctg tttcctgtgt gaaattgtta tccgctcaga gggcacaatc ctattccgcg
     3001 ctatccgaca atctccaaga cattaggtgg agttcagttc ggcgtatggc atatgtcgct
     3061 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3121 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3181 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3241 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3301 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3361 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3421 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3481 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3541 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3601 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3661 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3721 gatcctttga tcttttctac ggggtctgac gctctattca acaaagccgc cgtcccgtca
     3781 agtcagcgta aatgggtagg gggcttcaaa tcgtcctcgt gataccaatt cggagcctgc
     3841 ttttttgtac aaacttgttg ataatggcaa ttcaaggatc ttcacctaga tccttttaaa
     3901 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3961 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4021 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4081 tgctgcaatg ataccgcgag agccacgctc accggctcca gatttatcag caataaacca
     4141 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4201 tattaattgt tgctgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4261 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4321 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4381 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4441 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4501 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4561 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4621 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4681 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4741 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4801 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4861 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4921 gcgcacattt ccccgaaaag tgccagatac ctgaaacaaa acccatcgta cggccaagga
     4981 agtctccaat aactgtgatc caccacaagc gccagggttt tcccagtcac gacgttgt
//
