LOCUS       sequence-361773-        4924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Positive control for mosTI(unc-119). unc-119 rescue and germline 
            nuclear GFP expression after mosTI insertion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   El Mouridi S, Alkhaldi F, Frokjaer-Jensen C
  TITLE     Modular safe-harbor transgene insertion (MosTI) for targeted 
            single-copy and extrachromosomal array integration in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184.
  PUBMED    35900171
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184."
COMMENT     Sequence Label: pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2
FEATURES             Location/Qualifiers
     source          1..4924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..24
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8..24
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(648..681)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1193..1213
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      2154..2477
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(2836..2852)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2836..2852)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2849..2871)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(3024..3612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3104..3123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(3729..3753)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(3853..4644,4645..4713))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4476..4495
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4714..4818)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(4917..4924,1..15)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgacgttgta aaacgacggc cagtcatgca taatccgcac gcatctggaa taaggaagtg
       61 ccattccgcc tgacctaagt gcttttcgag atcgccaaac cggagaacga tcaggagaat
      121 gatgagtcgc cacaggagtc ggcaagatac gtgcgttata gatttgctcc aaactttttg
      181 aaactcaaaa cggtcggagc aactgtggaa ttcaaagtag gagacatccc aatccatcat
      241 ttccgaatga tcgaacgtca cttcttcaaa gatcgccttc tgaagtgttt tgactttgaa
      301 ttcggattct gtattccgaa ttcacgaaac aactgtgaac atatctatga gttccctcaa
      361 ctctctcaac aactcagtga gtcattattc taaaaagtac aattcaaaag actaatctct
      421 ttttcagtgg acgacatgat caacaatcca aacgagactc gttctgacag cttctatttc
      481 gtcgataaca aactcgtcat gcacaacaaa gccgactact catatgatgc ataaatcttc
      541 attgttgagt ttatcttgtt gatttttgaa taaattatca actctttact ttttaatggg
      601 ttatgaaata aataaacatt gaaaactgat aaacaacgtt catctctata acttcgtata
      661 gcatacatta tacgaagtta tggttttcac aacggcaaaa tatcagtttt taaaaaatta
      721 aaccataaaa caaataatat aacccaattt ttacatcaaa ccacaagaaa aaaatacatt
      781 tgggcccacg gataaagaaa ttaaaaaaat acatttttta aaggcgcacc gaattaaaat
      841 tcatttgggt cttaccgcgt ataccgtact ccgtttgttt gatcattttt gtcagcgctg
      901 gcggttgttt tttcatttca tttctgcttc aaagacgttt tctcgaataa tttttcgttt
      961 attctctttt ttaaaattaa tttctagccg taaatgttat aaattcaccc atttaacgca
     1021 aatttcatgg taatctcatg gaaaaatgca gtttctttgt taaagaaagc ttaaatagca
     1081 aaaattcccc gactttcccc aaaatcctgc tcgattttcc gttttctcat tgtattctct
     1141 cttaattaat tttatcgata atcaattgaa tgtttcagac agagaaaaaa tgccaaagaa
     1201 gaagagaaag gtgtcaaaag gagaagaact ctttaccgga gtcgtcccaa ttctcgtcga
     1261 gctcgacgga gacgtaaacg gacacaaatt ctcggtttcc ggagaaggag aaggaggtaa
     1321 gttttacgaa attctgaagc tcacaaaaca tggagtacat ttttcagatg ctacttatgg
     1381 aaaactcacc cttaaattca tttgcaccac cggaaagctc ccagtgccgt ggccgaccct
     1441 tgtgactaca ttctgctacg gagttcaatg cttttcccgc tacccagacc acatgaagcg
     1501 tcacgatttc ttcaaatcag ccatgccaga aggatacgtc caggagcgaa caattttctt
     1561 caaggacgat ggaaactaca agactcgtgc tgaagtcaag tttgaaggag atactctcgt
     1621 gaaccgcatt gagctcaagg taagtttata atctatgatc ttaatttggc aatggaaaaa
     1681 gttttttcag ggaatcgact tcaaagaaga tggaaacatt cttggacaca agctcgaata
     1741 caactataac tcgcacaacg tgtatatcat ggccgacaag caaaaaaatg gaatcaaggt
     1801 caacttcaag attcgccaca acatcgaaga cgggtcggtt caactcgctg atcactacca
     1861 gcagaacaca ccaattggag gtaagtttta ttccataata caaatatata ttgaacaata
     1921 atgcttttca gatggaccag tcctcctccc tgataatcac tacctttcca ctcaatccgc
     1981 tcttagcaag gatccaaatg agaaaagaga tcacatggtt cttctcgagt ttgtcaccgc
     2041 cgccggaatc acccacggaa tggacgagct ttacaagtcg agacgccgca aggctaatcc
     2101 aaccaaactt tctgaaaatg ccaagaaact tgccaaggag gtcgagaact gagatgcaaa
     2161 atcctttcaa gcattccctt cttctctatc actcttcttt ctttttgtca aaaaattctc
     2221 tcgctaattt atttgctttt ttaatgttat tattttatga ctttttatag tcactgaaaa
     2281 gtttgcatct gagtgaagtg aatgctatca aaatgtgatt ctgtctgatg tactttcaca
     2341 atctctcttc aattccattt tgaagtgctt taaacccgaa aggttgagaa aaatgcgagc
     2401 gctcaaatat ttgtattgtg ttcgttgagt gacccaacaa aaagaggaaa ctttattgtg
     2461 ccgccaagaa aaaagtctca caggaaacgg agaatctgtg cggcaatgtc ttatcatttg
     2521 aaacgcgccg tatcgatgct gacatcatgc ggctcccgtg ttagtcgaga agatgggtac
     2581 gggcctttat tcatccaaca ctctacggct tcttcaagag ctagtagggc accctgtagt
     2641 tggaaaggga ttatttcgta tggcgagccc ataccgtctt tcttgcggaa gcttaacacg
     2701 ataggaagtt ggatagtttc gaacgatggt tccatacgga acgctgtctc aaggatgagt
     2761 ctgacggagt gtaactcgaa ggctaggtgg aggctcagtg atgataagtc tgcgatggtg
     2821 gatgcatgtg tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca
     2881 caatcctatt ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcgt
     2941 atggcatatg tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3001 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3061 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3121 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3181 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3241 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3301 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3361 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3421 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3481 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3541 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3601 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctct attcaacaaa
     3661 gccgccgtcc cgtcaagtca gcgtaaatgg gtagggggct tcaaatcgtc ctcgtgatac
     3721 caattcggag cctgcttttt tgtacaaact tgttgataat ggcaattcaa ggatcttcac
     3781 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3841 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3901 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3961 accatctggc cccagtgctg caatgatacc gcgagagcca cgctcaccgg ctccagattt
     4021 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     4081 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     4141 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     4201 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     4261 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     4321 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     4381 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4441 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4501 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4561 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4621 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4681 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4741 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4801 acaaataggg gttccgcgca catttccccg aaaagtgcca gatacctgaa acaaaaccca
     4861 tcgtacggcc aaggaagtct ccaataactg tgatccacca caagcgccag ggttttccca
     4921 gtca
//
